- Human Carbonhydrate Antigen 125(CA125) ELISA Kit
- CUSABIO Technology LLC
- Pricing InfoSupplier PageView Company Product Page
- CSB-E04771h
- 96T
- 450 nm
- Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
- 50-100ul
- 1-5h
- ELISA
- ELISA Kit
- Homo sapiens (Human)
- mucin 16, cell surface associated
- CA125
- Cancer
Specifications/Features
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC16 bound in the initial step. The color development is stopped and the intensity of the color is measured.