- Human Interleukin 5,IL-5 ELISA Kit
- CUSABIO Technology LLC
- Pricing InfoSupplier PageView Company Product Page
- CSB-E04636h
- 96T
- 3.9 pg/ml-250 pg/ml
- 450 nm
- Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
- serum, plasma, tissue homogenates
- 0.97 pg/ml
- 50-100ul
- interleukin 5 (colony-stimulating factor, eosinophil)
- 1-5h
- ELISA
- ELISA Kit
- Homo sapiens (Human)
- interleukin 5
- EDF, IL-5, TRF
- Immunology
Specifications/Features
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.