Human IL-8 ELISA Kit ab46032 from Abcam

Product Specs
ItemHuman IL-8 ELISA Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab46032
Detection Range62.5 - 2000 pg/ml
Quantity1 96TESTS, 2 96TESTS
MethodELISA
ReactivityHuman
ApplicationsELISA
Gene NameCXCL8
TypeSandwich (quantitative)
Sensitivity25 pg/ml
NCBI Gene AliasesGCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
Detection TargetIL-8

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(1) Differential Effects of Nonsteroidal Anti-Inflammatory Drugs in an In Vitro Model of Human Leaky GutCellsFebruary 24, 2023d'Angelo M, Brandolini L, Catanesi M, Castelli V, Giorgio C, Alfonsetti M, Tomassetti M, Zippoli M, Benedetti E, Cesta MC, et al.chosen. 2.6. Measurement of Interleukin-8 Concentration The human interleukin-8 (IL-8) ELISA kit (ab46032, Abcam, Cambridge, UK) is designed for the quantitative measurement of IL-8 concentrations in the supernatants.

(2) CircRNA circFADS2 is under-expressed in sepsis and protects lung cells from LPS-induced apoptosis by downregulating miR-133aJournal of InflammationDecember 1, 2022Fang Niu, Xiaofeng Liang, Jindi Ni, Zhuye Xia, Lijing Jiang, Hong Wang, Hongjie Liu, Guofeng Shen, Xiang Licollected, and the contents of interleukins tumor necrosis factor (TNF)-α (ab181421), IL-6 (ab178013), IL-8 (ab46032), and (IL)-1β were analyzed using ELISA kits (Abcam, Cambridge, UK) following the manufacturer’s instructions. Dual‑luciferase reporter assay

(3) Inhibitory effects of isoliquiritin on an atopic dermatitis model through the CD177/JAK2/STAT pathway in vitro and in vivoAnnals of Translational MedicineSeptember 1, 2022Qing Wu, Xiumei Mo, Ying Lin, Junfeng Liu, Siqi Ye, Yu Zhang, Xingxing Fan, Dacan Chen, Fenggen Yanwere purchased from Abcam (Cambridge, MA, USA). In addition, ELISA kits for IL-6 (ab178013) and IL-8 (ab46032) for cell supernatants were obtained from Abcam. From Cell Signaling Technology, we procured antibodies

(4) Antioxidant Activities and Mechanisms of Tomentosin in Human KeratinocytesAntioxidants (Basel)May 18, 2022Seyoung Yang et al.times. 2.10. Enzyme-Linked Immunosorbent Assay (ELISA) for Target Proteins An interleukin (IL)-8 ELISA Kit (ab46032, Abcam, Cambridge, UK) was used to quantitate IL-8 levels following the manufacturer’s protocol. The

(5) Transient Receptor Potential Cation Channel Subfamily V Member 1 Expression Promotes Chemoresistance in Non-Small-Cell Lung CancerFrontiers in oncologyJanuary 1, 2022Li L, Chen C, Xiang Q, Fan S, Xiao T, Chen Y, Zheng D.serial dilutions of the culture supernatants were prepared for analysis using IL-8 Human ELISA Kits (ab46032, Abcam) according to the manufacturer’s instructions. Xenografts in Nude Mice Four to five weeks of

(6) Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular InflammationInternational journal of molecular sciencesNovember 18, 2021Shishkova DK, Velikanova EA, Bogdanov LA, Sinitsky MY, Kostyunin AE, Tsepokina AV, Gruzdeva OV, Mironov AV, Mukhamadiyarov RA, Glushkova TV, et al.supernatant were measured by enzyme-linked immunosorbent assay using the respective kits (ab178013 and ab46032, Abcam, Cambridge, UK) according to the manufacturer’s protocol. Alternatively, HCAECs and HITAECs cultured

(7) A possible pathogenic correlation between neutrophil elastase (NE) enzyme and inflammation in the pathogenesis of coronavirus disease 2019 (COVID-19)International ImmunopharmacologyNovember 1, 2021Sajad Karampoor, Khashayar Hesamizadeh, Faezeh Maleki, Mohammad Farahmand, Hamid Zahednasab, Rasoul Mirzaei, Helene Banoun, Farhad Zamani, Marzieh Hajibaba, Alireza Tabibzadeh, et al.levels of inflammatory (CRP; BOSTER BIOLOGICAL TECHNOLOGY, EK7040, IL-6; Abcam # ab46027, IL-8; Abcam # ab46032), and anti-inflammatory (IL-4; IBL International GmbH # BE58041, IL-10; IBL International GmbH # BE58101)

(8) Self-sustaining IL-8 loops drive a prothrombotic neutrophil phenotype in severe COVID-19JCI InsightSeptember 22, 2021Rainer Kaiser et al.snap-frozen on dry ice. IL-8 content of neutrophil supernatants was assessed using an IL-8 ELISA kit (Abcam, ab46032) and a plate reader (450 nm, Tecan Infinite F200). In vivo model of SARS-CoV-2 S-protein and LPS-induced

(9) Puerarin Inhibits Ferroptosis and Inflammation of Lung Injury Caused by Sepsis in LPS Induced Lung Epithelial CellsFrontiers in PediatricsAugust 4, 2021Baiye Xu, Haidao Wang, Zhen ChenDevices, Sunnyvale, CA, USA). ELISA Assay TNF-α ELISA kits (ab181421, Abcam, UK), IL-8 ELISA kit (ab46032, Abcam, UK) and IL-1β ELISA kit (ab214025, Abcam, UK) were used to measure the cell TNF-α, IL-8 and

(10) Chronic opioid use modulates human enteric microbiota and intestinal barrier integrityGut MicrobesJuly 27, 2021Angélica Cruz-Lebrón, Ramona Johnson, Claire Mazahery, Zach Troyer, Samira Joussef-Piña, Miguel E. Quiñones-Mateu, Christopher M Strauch, Stanley L. Hazen, Alan D. LevineIL-6, TNFα, IL-1β, and MIP1α (neat) (R&D Systems, HS600C, DTA00D, DLB50, DMA00), IL-8 (neat) (Abcam, ab46032), and CRP (1:100) (R&D Systems, DCRP00) were quantified following manufacturer’s instructions. LBP (1:100)

(11) MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a AxisOxidative Medicine and Cellular LongevityMay 25, 2021Changzhao Gao, Fei Yin, Ran Li, Qing Ruan, Chunyang Meng, Kunchi Zhao, Qingsan Zhusupernatant was determined for the levels of IL-1β (ab217608, Abcam, UK), IL-6 (ab178013, Abcam, UK), IL-8 (ab46032, Abcam, UK), TNF-α (ab181421, Abcam, UK), and other proinflammatory cytokines using corresponding ELISA

(12) Differentiation Antagonizing Non‐protein Coding RNA Knockdown Alleviates Lipopolysaccharide‐Induced Inflammatory Injury and Apoptosis in Human Chondrocyte Primary Chondrocyte Cells Through Upregulating miRNA‐19a‐3pOrthopaedic SurgeryFebruary 1, 2021Xue‐peng Li, Xu Wei, Shang‐quan Wang, Gang Sun, Ying‐chun Zhao, He Yin, Ling‐hui Li, Xun‐lu Yin, Kai‐ming Li, Li‐guo Zhu, et al.The ELISA kits, including a human IL‐1β kit (ab100562), a human IL‐6 kit (ab46027), a human IL‐8 kit (ab46032), and a human TNF‐α kit (ab46087), were purchased from Abcam (Cambridge, UK). The OD values at 450 nm

(13) Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine InteractionsInternational journal of molecular sciencesOctober 28, 2020Shishkova D, Markova V, Sinitsky M, Tsepokina A, Frolov A, Zagorodnikov N, Bogdanov L, Kutikhin A.supernatant were analysed utilising respective enzyme-linked immunosorbent assay kits (ab178013 and ab46032, Abcam, Cambridge, UK), according to the manufacturer’s protocols. The colorimetric detection was carried

(14) LncRNA OIP5‑AS1 aggravates house dust mite‑induced inflammatory responses in human bronchial epithelial cells via the miR‑143‑3p/HMGB1 axisMolecular Medicine ReportsSeptember 25, 2020Xing‑Jun Cai, Lin‑Hui Huang, Yi‑Ke Zhu, Yi‑Jiang Huangof TNF‑α, IL‑6 and IL‑8 were evaluated using commercial ELISA kits (cat. nos. ab181421, ab178013 and ab46032, respectively; all from Abcam) according to the manufacturer's instructions. Annexin V‑Fluorescein isothiocyanate

(15) (Sub)populations of extracellular vesicles released by TNF‐α –triggered human endothelial cells promote vascular inflammation and monocyte migrationJournal of Extracellular VesiclesSeptember 1, 2020Baharak Hosseinkhani, Nynke M.S. van den Akker, Daniel G.M. Molin, Luc Michielssystem. ELISA Quantification of ICAM-1 (ab100640, Abcam), inflammatory cytokines [IL-6 (ab46027), IL-8 (ab46032), CCL-2 (MCP1, ab179886)], chemokines [CCL-4 (MIP1β, ab100597), CCL-5 (RANTES, ab174446) and CXCL-10

(16) Retinoic Acid-Platinum (II) Complex [RT-Pt(II)] Protects Against Rheumatoid Arthritis in Mice via MEK/Nuclear Factor kappa B (NF-κB) Pathway DownregulationMedical Science MonitorAugust 3, 2020Ziqiang Cui, Yaping Lin, Yuhong Liu, Ling Cao, Li Cui15 min to obtain the supernatants. The levels of cytokines – IL‑1b (Ab100562), IL-6 (Ab46027), IL-8 (Ab46032), MMP-1 (Ab100603), and MMP-13 (Ab100605) – were assessed using commercially available ELISA kits (Abcam,

(17) lncRNA‑MM2P downregulates the production of pro‑inflammatory cytokines in acute gouty arthritisMolecular Medicine ReportsJuly 9, 2020Xifeng Zhang, Ying Zou, Jiangxia Zheng, Senguo Ji, Xiuzhen Wen, Feng Ye, Ju Liu, Xueyong Li, Jin Lei, Mingliang Qiukits purchased from Abcam: Human IL-1β ELISA kit (cat. no. ab46052), human IL-8 ELISA kit (cat. no. ab46032) and human TNFα ELISA kit (cat. no. ab181421). Generation of inflammatory gene products (IL-1β, IL-8

(18) Interleukin-6 and Lymphocyte Count Associated and Predicted the Progression of Frailty Syndrome in Prostate Cancer Patients Undergoing Antiandrogen TherapyCancers (Basel)June 29, 2020Cristina Buigues, Rut Navarro-Martínez, Vanessa Sánchez-Martínez, María Serrano-Carrascosa, José Rubio-Briones, Omar Cauliaccording to the manufacturer’s instructions (TNF-a (ab100654), IL-6 (ab46042), IL-1 beta (ab46042), IL-8 (ab46032) Human ELISA Kit, Abcam). We also counted the five-part Cancers 2020, 12, 1716 12 of 17 white blood cell

(19) Blood Neutrophil-to-Lymphocyte Ratio and Urine IL-8 Levels Predict the Type of Bacterial Urinary Tract Infection in Type 2 Diabetes Mellitus PatientsInfection and Drug ResistanceJune 24, 2020Fatemeh Saheb Sharif-Askari, Narjes Saheb Sharif-Askari, Adnane Guella, Ali Alabdullah, Hour Bashar Al Sheleh, Afnan Maher Hoory AlRawi, Enad Sami Haddad, Qutayba Hamid, Rabih Halwani, Rifat Hamoudilevels for T2DM were assessed using ELISA assay according to the manufacture’s instructors (IL-8, Cat # ab46032 Abcam, Cambridge, MA, USA). Each sample was assayed in triplicate and values were expressed as the mean

(20) Calcium phosphate bions cause increased release of interleukin-6 and interleukin-8 in primary arterial endothelial cells regardless of their shapeNauchno-prakticheskii zhurnal «Patogenez»June 16, 2020А.Г. Кутихин, Д.К. Шишкова, М.Ю. Синицкий, Е.А. Великановаанализа посредством соответствующих наборов компании Abcam (Соединенное Королевство): ab46052, ab46027, ab46032, ab46034, ab46143, ab64708, ab46087, ab46025 в соответствии с инструкциями производителя. Выбор указанных

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Human IL-8 ELISA Kit from Abcam

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Human IL-8 ELISA Kit from Abcam

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