Fig 1: UV–vis absorption spectra of (a) 4-nitrophenol, (b) 4-NP-loaded aptamer/cDNA-MMSNs and (c) the supernatant after 4-NP-loaded aptamer/cDNA-MMSNs reacted with 1.0 ng mL-1 of CEA.
Fig 2: Effects of (A) aptamer concentration in the preparation of magnetic aptasensor and (B) reaction time between aptamer and target CEA on the spectrometric absorbance of aptasensor. (Note that each data point represents the average value obtained from three measurements. The error bars represent the 95% confidence interval of the mean for y-axis concentrations).
Fig 3: Variation in response of rGO/MEL/antibody/BSA devices for (a) CEA, (b) CYFRA 21-1.
Fig 4: The specificity of the spectrometric aptasensing platform with respect to target CEA, AFP, NSE, hCG, CA 125, and CA 15-3. (Note that each data point represents the average value obtained from three measurements. The error bars represent the 95% confidence interval of the mean for y-axis concentrations).
Fig 5: (a,b) Comparative response of rGO/MEL/antibody/BSA and rGO/Triazine/antibody/BSA for (a) CEA, (b) CYFRA 21-1, (c,d) response of (c) rGO/MEL/anti-CEA/BSA to 1 pg/mL to 800 ng/mL of CEA, (d) response of rGO/MEL/anti-CYFRA 21-1/BSA/ to 1 pg/mL to 800 ng/mL of CYFRA 21-1 at room temperature. (e) Cross-reactivity results, (f) stability test results.
Supplier Page from Abcam for Human Carcino Embryonic Antigen ELISA Kit (CEA)