Anti-HSP60 IgG/A/M (human), ELISA kit from Enzo Life Sciences, Inc.


This assay allows for reproducible, accurate and precise determination of IgG, IgA and IgM antibodies (total) to human Hsp60 in serum. The ELISA kit uses recombinant human Hsp60 bound to the wells of the plate to bind anti-human Hsp60 antibodies present in human serum. The captured anti-human Hsp60 antibodies are detected with a HRP conjugated goat polyclonal antibody specific for human IgG, IgA and IgM molecules. The assay is developed with tetramethylbenzidine substrate pro ducing a blue color in proportion to the amount of captured anti -human Hsp60 antibodies. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450 nm