ELISA MAX(TM) Standard Set Human IFN-gamma 430101 from BioLegend

Product Specs
ItemELISA MAX(TM) Standard Set Human IFN-gamma
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number430101
ApplicationsELISA
Detection Range15.6-2000 pg/ml
MethodELISA
Quantity5 Plates
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFG, IFI, IMD69
SpeciesHuman
Molecule NameIFN-gamma

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Granulocyte-Macrophage Colony-Stimulating Factor Influence on Soluble and Membrane-Bound ICOS in Combination with Immune Checkpoint BlockadeCancer immunology researchAugust 3, 2023Li X, Li J, Zheng Y, Lee SJ, Zhou J, Giobbie-Hurder A, Butterfield LH, Dranoff G, Hodi FS.and IFNγ in cell culture supernatants, IL12 p40 (430701), IL12 p70 (431701), TNFα (430201), and IFNγ (430101) ELISA kits purchased from BioLegend were used according to the manufacturer's protocols, respectively.

(2) PD-1/LAG-3 bispecific antibody potentiates T cell activation and increases antitumor efficacyFrontiers in ImmunologyNovember 28, 2022Ning Shi, Yangyihua Zhou, Yujun Liu, Ran Zhang, Xingjun Jiang, Caiping Ren, Xiang Gao, Longlong Luo480130) and ELISA MAX™ Standard Set Human kit IFN-γ) were purchased from Biolegend corporation (Cat. No.: 430101); Cytotoxicity Detection Kit PLUS (LDH) were purchased from Roche(Cat. No. 04744934001); Simple PlexTM

(3) Antibody-dependent cellular cytotoxicity-null effector developed using mammalian and plant GlycoDelete platformScientific reportsNovember 8, 2022Kang CE, Lee S, Ahn T, Seo DH, Ko BJ, Jung M, Lee J, Kim JY, Kim WT.the plate at 1500 rpm for 20 min. The levels of IL-2 and INF-γ were measure by ELIZA (#431081 and #430101, respectively, Biolegend). HEK 293 T cell (ATCC), breast cancer cell lines MCF7 (PD-L1 (−), Korean Cell

(4) Serum 25-hydroxycholesterol levels are increased in patients with coronavirus disease 2019â€ J Clin LipidolNovember 6, 2022Takumi Asano, Tetsuji Wakabayashi, Yasuyuki Kondo, Kenta Okada, Daisuke Yamamuro, Yukiko Koga, Kiyonori Oka, Momoe Sakurai, Nagisa Sawayama, Manabu Takahashi, et al.cytokine concentrations using ELISA MAX Standard Sets (BioLegend; human IL-6, #430501; human IFN-γ, #430101), according to the manufacturer's instructions. Cell Culture Experiments Blood was collected from

(5) Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cellsSTAR ProtocolsJune 9, 2022Chia-Hung Christine Hsiao, Andrew J. Wiemer,Miltenyi Biotec,Cat#130-092-892 MS columns,Miltenyi Biotec,Cat#130-042-201 ELISA MAX™ Standard Set Human IFN-γ,BioLegend,Cat#430101 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, K562 cells

(6) Itaconate and derivatives reduce interferon responses and inflammation in influenza A virus infectionPLOS PathogensJanuary 13, 2022Aaqib Sohail et al.using ELISA MAX Deluxe Sets (Biolegend; IL-1β cat. no. 437004; IL-6, 430501; TNF-α, 430201; IFN-γ, 430101) as per the manufacturer’s instructions. Briefly, capture antibody was used to pre-coat the wells O/N.

(7) Microbiota metabolite butyrate constrains neutrophil functions and ameliorates mucosal inflammation in inflammatory bowel diseaseGut MicrobesSeptember 8, 2021Gengfeng Li, Jian Lin, Cui Zhang, Han Gao, Huiying Lu, Xiang Gao, Ruixin Zhu, Zhitao Li, Mingsong Li, Zhanju LiuUSA). The following ELISA assay kits for human IL-6 (Cat: 430504), TNF-α (Cat: 430204), IFN-γ (Cat: 430101), IL-8 (Cat: 431504), S100A8/9 (Cat: 439707), lipocalin-2 (LCN2, Cat: 443407), and proinflammatory chemokine

(8) Engineering genetic devices for in vivo control of therapeutic T cell activity triggered by the dietary molecule resveratrolProceedings of the National Academy of Sciences of the United States of AmericaAugust 24, 2021Yang L, Yin J, Wu J, Qiao L, Zhao EM, Cai F, Ye H.the supernatants were collected and analyzed for human hIL-2 or hIFN-γ by ELISA (BioLegend, 431804 or 430101). Cytotoxicity Assay of RESrep-CAR or RESind-CAR Devices in Engineered Primary Human T Cells. CD19+

(9) HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responsesCell host & microbeMarch 10, 2021Clayton KL, Mylvaganam G, Villasmil-Ocando A, Stuart H, Maus MV, Rashidian M, Ploegh HL, Walker BD.overnight co-culture, the IFN-γ in the culture supernatants was measured via ELISA (Biolegend, Cat#430101). IFN-γ values were interpolated from the standard curve and normalized based on differences in infection

(10) The T Cell Repertoires from Nickel Sensitized Joint Implant Failure PatientsInternational Journal of Molecular SciencesFebruary 28, 2021Lan Chen, Yan Zhang, Karin Pacheco, Shaodong DaiUSA). 4.4. IFNγ Measurement IFNγ was measured using ELISA MAX Standard Set Human IFN-γ (Biolegend #430101). 4.5. Cell Staining and Single-Cell Sequencing The sorted PBMCs were stained based on a Biolegend recommended

(11) HLA-DR15 Molecules Jointly Shape an Autoreactive T Cell Repertoire in Multiple SclerosisCellNovember 25, 2020Wang J, Jelcic I, Mühlenbruch L, Haunerdinger V, Toussaint NC, Zhao Y, Cruciani C, Faigle W, Naghavian R, Foege M, et al."CD4 MicroBeads, human",Miltenyi Biotec,Cat# 130-045-101 ELISA MAX Standard Set Human IFN-γ,Biolegend,Cat# 430101 LEGENDplex Human T Helper Cytokine Panels,Biolegend,"Cat# 740001, RRID:AB_2784515" "T Cell Activation/Expansion Kit, human

(12) convertibleCARs: A chimeric antigen receptor system for flexible control of activity and antigen targetingCommunications biologyJune 9, 2020Landgraf KE, Williams SR, Steiger D, Gebhart D, Lok S, Martin DW, Roybal KT, Kim KC.could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101). The MicAbody binding curve data were generated by ProMab Biotechnologies, Inc. (Richmond, CA). 3 ×

(13) Sphingosine Kinase Blockade Leads to Increased Natural Killer T Cell Responses to Mantle Cell LymphomaCellsApril 21, 2020Michael S. Lee, Wenji Sun, Tonya J. Webbprimary NKT cells. After co-culture, IFN-γ levels in the supernatant were determined by ELISA (BioLegend, 430101). 2.7. Cytotoxicity Assays MCL cell lines were incubated with primary human NKT cells at the indicated

(14) Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFvFrontiers in cell and developmental biologyJanuary 1, 2020Ping Y, Li F, Nan S, Zhang D, Shi X, Shan J, Zhang Y.the supernatant were tested for IL2 and IFN-γ with cytokine-specific ELISA kits (Biolegend, Cat. Nos. 431804 and 430101) according to the instructions. Cell Immunofluorescence Lentivirus Production and T Cell Transduction PD-1-overexpressing

(15) Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of ImmunotherapiesFrontiers in oncologyJanuary 1, 2020Saraiva DP, Matias AT, Braga S, Jacinto A, Cabral MG.number: 304026), store at 4°COptional: Human IFN-gamma ELISA MAX™ Standard (Biolegend, catalog number: 430101), store at 4°C. Equipment MicropipettesAutomatic pipettesMultichannel pipettesCentrifugeBiosafety

(16) Split chimeric antigen receptor-modified T cells targeting glypican-3 suppress hepatocellular carcinoma growth with reduced cytokine releaseTherapeutic advances in medical oncologyJanuary 1, 2020Liu X, Wen J, Yi H, Hou X, Yin Y, Ye G, Wu X, Jiang X.cytokine release, including IFN-γ, IL-2, and IL-6, were measured by corresponding ELISA kit (BioLegend, #430101, #431801 and #430501). Cytotoxicities were determined by measuring lactate dehydrogenase (LDH) activity

(17) A secreted PD-L1 splice variant that covalently dimerizes and mediates immunosuppressionCancer immunology, immunotherapy : CIIMarch 1, 2019Mahoney KM, Shukla SA, Patsoukis N, Chaudhri A, Browne EP, Arazi A, Eisenhaure TM, Pendergraft WF 3rd, Hua P, Pham HC, et al.

(18) PARP inhibition enhances tumor cell-intrinsic immunity in ERCC1-deficient non-small cell lung cancerThe Journal of clinical investigationMarch 1, 2019Chabanon RM, Muirhead G, Krastev DB, Adam J, Morel D, Garrido M, Lamb A, Hénon C, Dorvault N, Rouanne M, et al.

(19) Porcine Circovirus Type 2 Suppresses IL-12p40 Induction via Capsid/gC1qR-Mediated MicroRNAs and SignalingsJournal of immunology (Baltimore, Md. : 1950)July 15, 2018Du Q, Wu X, Wang T, Yang X, Wang Z, Niu Y, Zhao X, Liu SL, Tong D, Huang Y.secretion were measured by commercial enzyme-linked immunosorbent assay (ELISA) kit (P1240, R&D, and 430101, BioLegend) according to the manufacturer’s instructions. Flow cytometry The PBMCs and PAMs were harvested

(20) TNFR2/BIRC3-TRAF1 signaling pathway as a novel NK cell immune checkpoint in cancerOncoimmunologyJanuary 1, 2018Ivagnès A, Messaoudene M, Stoll G, Routy B, Fluckiger A, Yamazaki T, Iribarren K, Duong CPM, Fend L, Caignard A, et al.

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ELISA MAX(TM) Standard Set Human IFN-gamma from BioLegend

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ELISA MAX(TM) Standard Set Human IFN-gamma from BioLegend

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