Alpha Ketoglutarate (alpha KG) Assay Kit ab83431 from Abcam

Product Specs
ItemAlpha Ketoglutarate (alpha KG) Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab83431
Detection Range0.01 - 10 nmol/well
SensitivityEqual to 0.01 nmol/well
Assay Time0h 30m
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids
MethodFuncS
Quantity100 Test

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(1) Glutaminase-mediated glutamate metabolism is critical for maintaining Th17/Treg balanceCentral European Journal of ImmunologyMarch 31, 2026Xiaohan Jin, Jinglong Taomethod. The glutamine ELISA kit (UNEB0091) was purchased from AssayGenie. Glutamate (ab83389), α-KG (ab83431) and GSH/GSSG (ab138881) ELISA kits were purchased from Abcam. The experiment was performed according

(2) Reversible arginine methylation regulates mitochondrial IDH2 activity: coordinated control by CARM1 and KDM3A/4ACell Death DisFebruary 2, 2026Yena Cho, Jessica Winarto, Dae-Geun Song, Dong Hee Na, Kyo Bin Kang, Su-Nam Kim, Yong Kee Kimcalibrations. Quantification of metabolites α-KG levels were analyzed using an Alpha Ketoglutarate Assay Kit (Abcam, ab83431) and the NADPH/NADP+ ratio was determined using an NADP/NADPH Assay Kit (Abcam, ab65349), according

(3) IGF2BP3-dependent glutamine/BCAA metabolic rewiring rejuvenates aged human adipose-derived stem cells for enhanced tissue regenerationCell DiscoveryJanuary 20, 2026Zichao Li, Lin Feng, Xinxin Wei, Huichen Li, Yifu Zhu, Hongtao Wang, Jiaqi Liu, Liang Luo, Zhao Zheng, Baoqiang Song, et al.glutamate (#ab83389; Abcam), succinate (#ab204718; Abcam), fumarate (#ab102516; Abcam), and α-KG (#ab83431; Abcam) assay kits, respectively, according to the manufacturer’s protocol. The absorbance measured

(4) METTL3-mediated GLUD1 m6A Modification Promotes Hydrogen Peroxide-induced Mitochondrial Dysfunction in Human Nucleus Pulposus Cells Via the Glutamate/α-KG Metabolic AxisJ Musculoskelet Neuronal InteractJanuary 1, 2026Ming Bai, Yimin Wu, Yang Zhang, Haibin Zhang, Peng Wang, Shuwen Liα-KG, and ATP were quantified using a glutamate assay kit (ab83389), an alpha-ketoglutarate assay kit (ab83431), and an ATP assay kit (ab83355) (all from Abcam, Waltham, MA, USA) according to the manufacturer’s

(5) Alpha-ketoglutarate promotes random-pattern skin flap survival by enhancing angiogenesis via PI3K/Akt/HIF-1α signaling pathwayCell RegenDecember 22, 2025Jiefeng Huang, Shuangmeng Jia, Yitong Ji, Yingjia Zhu, Yishu Lu, Yiming Tang, Jiajie Yang, Guangpeng Liu, Lei Cui, Shuaijun Li13,000g for 10 min. Levels of α-KG and Gln in supernatant were measured using α-KG assay kit (Abcam, ab83431), and glutamine assay kit (Abcam, ab197011), respectively. The concentration of Gln and α-KG was calculated

(6) Lactate as a metabolic–epigenetic signal linking high-intensity interval training (HIIT) to miRNA-Centered remodeling of the skeletal muscle methylome and transcriptomeRedox BiologyDecember 1, 2025Lei Zhou, Soroosh Mozaffaritabar, Kumpei Tanisawa, Takuji Kawamura, Mitsuru Higuchi, Istvan Boldogh, Xueqing Ba, Sataro Goto, George Brooks, Yaodong Gu, et al.gastrocnemius muscle were measured using assay kits (Abcam; lactate kit ab65330, pyruvate kit ab65342, αKG kit ab83431, NAD/NADH kit ab176723). Approximately 20 ± 2 mg of homogenized tissue from the pre-mixed gastrocnemius

(7) O-GlcNAcylation of glutaminase isoform KGA inhibits ferroptosis through activation of glutaminolysis in hepatoblastomaCell Death DiscovApril 9, 2025Sijia Fang, Guoqing Zhu, Yi Xie, Miao Ding, Ni Zhen, Jiabei Zhu, Siwei Mao, Xiaochen Tang, Han Wu, Qi Zhang, et al.Beyotime). Alpha-ketoglutarate (α-KG) levels were determined using the alpha-ketoglutarate test kit (ab83431, Abcam, Hong Kong, China). GSH and GSSG levels were quantified using GSH and GSSG assay kits (RK05819,

(8) Fucoxanthinol Mitigates the Cytotoxic Effect of Chlorpyrifos and MPTP on the Dopaminergic Differentiation of SH-SY5Y Human Neuroblastoma CellsJ Mol NeurosciApril 8, 2025Ekramy M. Elmorsy, Ayat B. Al-Ghafari, Huda A. Al Doghaither, Mona M. Elghareeb, Mouhamed Alsaqaticoncentrations were measured utilizing the alpha-ketoglutarate assay kit (colorimetric) from Abcam (Catalog No. ab83431), in accordance with the manufacturer’s instructions. Initially, samples underwent deproteinization

(9) PER2 interaction with HSP70 promotes cuproptosis in oral squamous carcinoma cells by decreasing AKT stabilityCell Death DisMarch 20, 2025Wenguang Yu, Shilin Yin, Hong Tang, Hengyan Li, Zhiwei Zhang, Kai Yangand α-ketoglutarate was performed using a Fumarate Assay Kit (MAK060, Sigma, USA) and α-KG Assay Kit (ab83431, Abcam) according to the instructions provided. The absorbance values of fumarate and α-ketoglutaric

(10) Downregulation of malic enzyme 3 facilitates progression of gastric carcinoma via regulating intracellular oxidative stress and hypoxia-inducible factor-1α stabilizationCellular and Molecular Life SciencesDecember 1, 2024Yingying Huang, Yan Yang, Xiangliu Chen, Siying Zeng, Yiran Chen, Haiyong Wang, Xiadong Lv, Xun Hu, Lisong Tengmanufacturer’s instructions, the α-KG in cells was measured using Abcam α-ketoglutarate Assay Kit (ab83431). Statistical analysis All data were analyzed using GraphPad Prism software (GraphPad Prism 8.0) and

(11) IDH2 and GLUD1 Depletion Arrests Embryonic Development through an H4K20me3 Epigenetic Barrier in Porcine Parthenogenetic EmbryosZoological ResearchNovember 18, 2024Cheng-Lin Zhan, Chungbuk National University Department of Animal Science, Qin-Yue Lu, Song-Hee Lee, Xiao-Han Li, Ji-Dam Kim, Gyu-Hyun Lee, Jae-min Sim, Hyeon-Ji Song, Ying-Yan Jin, et al.400 embryos/group were used to detect α-KG content using an Alpha Ketoglutarate (alpha KG) assay kit (ab83431; Abcam, UK). Statistical analysis All statistical analyses were performed using one-way analysis of variance

(12) OXGR1-Dependent (Pro)Renin Receptor Upregulation in Collecting Ducts of the Clipped Kidney Contributes to Na(+) Balance in Goldblatt Hypertensive MiceInternational journal of molecular sciencesSeptember 18, 2024Cárdenas P, Nuñez-Allimant C, Silva K, Cid-Salinas C, León AC, Vallotton Z, Lorca RA, Oliveira LCG, Casarini DE, Céspedes C, et al.Measurements of α-Ketoglutarate Tissue α-ketoglutarate was measured with Abcam α-ketoglutarate Assay Kit ab83431 (Abcam, Cambridge, UK) according to the manufacturer’s instructions. 4.13. Ang II Medullary Kidney

(13) IDH2 regulates macrophage polarization and tumorigenesis by modulating mitochondrial metabolism in macrophagesMolecular MedicineSeptember 10, 2024Sung Woo Lee, Soyoon Kim, Bokyung Kim, Jung Bae Seong, Young-Ho Park, Hong Jun Lee, Dong Kyu Choi, Eunbyul Yeom, Dong-Seok Lee72 h of culturing, cells were washed with PBS and resuspended in 500 μL ice-cold α-KG Assay Buffer (ab83431, Abcam; Cambridge, UK) according to the manufacturer’s instructions. Cells were quickly homogenized

(14) Recent progress of methods for cuproptosis detectionFrontiers in Molecular BiosciencesSeptember 4, 2024Ligang Zhang, Ruiting Deng, Raoqing Guo, Yawen Jiang, Yichen Guan, Caiyue Chen, Wudi Zhao, Guobin Huang, Lian Liu, Hongli Du, et al.(2018) Pyruvate,Elabscience #E-BC-K130-S,Colorimetric,Obaid et al. (2022) α-ketoglutarate,Abcam #ab83431,Fluorometric,Wang et al. (2020b) Acetyl-CoA,Abcam #ab87546,Fluorescent,Puca et al. (2021) Succinate,Abcam

(15) Branched-chain amino acid transaminase 1 confers EGFR-TKI resistance through epigenetic glycolytic activationSignal Transduction and Targeted TherapyAugust 15, 2024Tao Zhang, Zilu Pan, Jing Gao, Qingqing Wu, Gang Bai, Yan Li, Linjiang Tong, Fang Feng, Mengzhen Lai, Yingqiang Liu, et al.Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit (Biovision, K564-100) and Alpha Ketoglutarate (alpha KG) Assay Kit (Abcam, ab83431) respectively, following the manufacturer’s instructions. The absorbance was measured using a microplate

(16) A positive feedback inhibition of isocitrate dehydrogenase 3β on paired-box gene 6 promotes Alzheimer-like pathologySignal Transduction and Targeted TherapyApril 29, 2024Xin Wang, Qian Liu, Hai-tao Yu, Jia-zhao Xie, Jun-ning Zhao, Zhi-ting Fang, Min Qu, Yao Zhang, Ying Yang, Jian-Zhi WangAbcam). Alpha Ketoglutarate levels were determined with an Alpha-Ketoglutarate (alpha KG) Assay Kit (ab83431, Abcam). Additionally, ATP levels were measured utilizing the ATP Assay Kit (S0027, Beyotime). The Deproteinizing Sample Preparation Kit—TCA

(17) IDH1 mutation inhibits differentiation of astrocytes and glioma cells with low oxoglutarate dehydrogenase expression by disturbing α-ketoglutarate-related metabolism and epigenetic modificationLife MetabolismApril 1, 2024Yuanlin Zhao, Ying Yang, Risheng Yang, Chao Sun, Xing Gao, Xiwen Gu, Yuan Yuan, Yating Nie, Shenhui Xu, Ruili Han, et al.primary astrocytes and glioma cells cultured in 6-well plates were measured using α-KG Assay Kit (Abcam, ab83431). The concentrations of D-2HG in glioma cells were measured using D-2HG Assay Kit (Colorimetric) (Abcam,

(18) CircCPA4 induces ASCT2 expression to promote tumor property of non‐small cell lung cancer cells in a miR‐145‐5p‐dependent mannerThoracic CancerApril 1, 2024Zhenhua Zhang, Weiliang Liu, Tao Huang, Junyan Li, Hui Hu, Xinyu Xu, Zhigang Fanreader. Intracellular α‐KG level was analyzed with the application of the alpha ketoglutarate assay kit (ab83431, Abcam). Briefly, NSCLC cells were harvested and resuspended in 500 μL of alpha KG assay buffer. Cell

(19) ETV2 regulating PHD2-HIF-1α axis controls metabolism reprogramming promotes vascularized bone regenerationBioact MaterMarch 22, 2024HaoRan Du, Bang Li, Rui Yu, Xiaoxuan Lu, ChengLin Li, HuiHui Zhang, Fan Yang, RongQuan Zhao, WeiMin Bao, Xuan Yin, et al.curve. α-Ketoglutarate (α-KG) content in the supernatant was determined using the Alpha Ketoglutarate Assay kit (ab83431, Abcam) through colorimetry. According to the operating manual, the α-KG standard was gradient-diluted

(20) Psat1-generated α-ketoglutarate and glutamine promote muscle stem cell activation and regenerationGenes & DevelopmentFebruary 1, 2024Veronica Ciuffoli, Xuesong Feng, Kan Jiang, Natalia Acevedo-Luna, Kyung Dae Ko, A. Hong Jun Wang, Giulia Riparini, Mamduh Khateb, Brian Glancy, Stefania Dell'Orso, et al.an automated cytometer (Cellometer Vision, Nexcelom) and immediately processed for α-KG assay (Abcam ab83431) following the manufacturer's instructions. α-KG values were normalized to total protein levels. Glutamine/glutamate

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Alpha Ketoglutarate (alpha KG) Assay Kit from Abcam

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Alpha Ketoglutarate (alpha KG) Assay Kit from Abcam

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