Anti-Beta Lactamase antibody [8A5.A10] ab12251 from Abcam

Product Specs
ItemAnti-Beta Lactamase antibody [8A5.A10]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab12251
Antibody TypePrimary
IsotypeIgG1
ApplicationsELISA, Western blot
Quantity500 UG
HostMouse, Nude mouse
ClonalityMonoclonal
Conjugate/TagUnconjugated
Concentration1.4 mg/ml
Clone8A5.A10
Gene NameblaNDM-1
ReactivityEscherichia coli
TargetBeta Lactamase

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Exploiting the aggregation propensity of beta-lactamases to design inhibitors that induce enzyme misfoldingNature CommunicationsSeptember 9, 2023Ladan Khodaparast, Laleh Khodaparast, Guiqin Wu, Emiel Michiels, Rodrigo Gallardo, Bert Houben, Teresa Garcia, Matthias De Vleeschouwer, Meine Ramakers, Hannah Wilkinson, et al.codes The antibodies and antibiotic product codes used are as follows: monoclonal anti-TEM (Abcam, UK ab12251-8A5A10) 0.5 µg/mL, polyclonal rabbit anti-SHV (custom-made by Eurogentec, Belgium) 1 µg/mL, chicken

(2) Legionella longbeachae Regulates the Association of Polyubiquitinated Proteins on Bacterial Phagosome with Multiple DeubiquitinasesMicrobiology spectrumFebruary 15, 2023Shi Y, Liu H, Ma K, Luo ZQ, Qiu J.50430-2-AP; 1:5,000), anti-isocitrate dehydrogenase (ICDH) (1:10,000) (49), anti-TEM (Abcam; catalog no. ab12251; 1:3,000), anti-LLO_2238 (1:2,000), and anti-LLO_1014 (1:2,000). Rabbit polyclonal antibodies against

(3) Legionella longbeachae effector protein RavZ inhibits autophagy and regulates phagosome ubiquitination during infectionPloS oneJanuary 1, 2023Shi Y, Liu H, Ma K, Luo ZQ, Qiu J.source and dilutions for other primary antibodies used in this study were as follows: anti-TEM1 (Abcam, ab12251, 1:3,000), anti-Flag (Sigma, F1804, 1:3,000), anti-LC3 (Sigma, SAB5701328, 1:3,000), anti-GFP (Sigma,

(4) In Vivo Secretion of β-Lactamase-Carrying Outer Membrane Vesicles as a Mechanism of β-Lactam Therapy FailureMembranesOctober 23, 2021Bielaszewska M, Daniel O, Nyč O, Mellmann A.Germany), and stained with a mouse monoclonal anti-β-lactamase antibody (Abcam, Cambridge, UK, Cat# ab12251, RRID:AB_298974) and goat anti-mouse IgG conjugated with 10 nm gold (Abcam, Cambridge, UK, Cat# ab39619,

(5) Crystal structure of bacterial cytotoxic necrotizing factor CNF _Y reveals molecular building blocks for intoxicationThe EMBO JournalFebruary 15, 2021Paweena Chaoprasid et al.Sigma No. A2228‐100UL, anti‐Flag‐M2 from Sigma No. F3165‐1MG, anti‐β‐lactamase (TEM) from Abcam No. ab12251, anti‐GFP from Sigma No. 11814460001, and anti‐IgG from Cell Signalling No. 7076S. Cloning, expression,

(6) Programmable Extracellular Vesicles for Macromolecule Delivery and Genome ModificationsDevelopmental CellDecember 1, 2020Xiaojuan Zhang, Quanbin Xu, Zhike Zi, Zeyu Liu, Chun Wan, Lauren Crisman, Jingshi Shen, Xuedong Liual., 2017.",N/A Antibodies,, Mouse anti-VSV-G,Kerafast,"8G5F11, Cat#EB0010" Mouse anti-BlaM,Abcam,Ab12251 Rabbit anti-GFP,Cell Signaling Technology,D5.1 Mouse anti-Myc tag,Cell Signaling Technology,"B911,

(7) Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1PLOS Neglected Tropical DiseasesApril 1, 2020Arindam Naha et al.dilution of mouse monoclonal anti-DnaK (8E2/2, Enzo Life Sciences) or 1:500 dilution of anti-β-lactamase (ab12251, Abcam) antibody. After three washes for a total of 30 mins with TBST buffer, each blot was incubated

(8) CexE Is a Coat Protein and Virulence Factor of Diarrheagenic PathogensFrontiers in microbiologyJanuary 1, 2020Rivas ZP, Talbot KM, Merselis LC, McCormack RM, Adkins B, Munson GP.TBS-Blotto with 0.05% vol./vol. Tween20 (Pilonieta et al., 2007). Anti-DnaK (ab69617) and anti-β-lactamase (ab12251) antibodies were purchased from AbCam and used at dilutions of 1:10,000. Anti-FLAG (F1804) was purchased

(9) Integrin but not CEACAM receptors are dispensable for Helicobacter pylori CagA translocationPLOS PathogensOctober 26, 2018Qing Zhao, Benjamin Busch, Luisa Fernanda Jiménez-Soto, Hellen Ishikawa-Ankerhold, Steffen Massberg, Laurent Terradot, Wolfgang Fischer, Rainer Haaspreviously [5, 53]. The mouse monoclonal antibody against TEM-1 β-lactamase was obtained from Abcam (ab12251). For immunoblotting the following antibodies were used: ITGAv, (EPR16800, abcam), ITGB1 (LM534, EMD

(10) Probing the quality control mechanism of the Escherichia coli twin-arginine translocase with folding variants of a de novo–designed heme proteinThe Journal of Biological ChemistryMarch 20, 2018George A. Sutherland, Katie J. Grayson, Nathan B. P. Adams, Daphne M. J. Mermans, Alexander S. Jones, Angus J. Robertson, Dirk B. Auman, Amanda A. Brindley, Fabio Sterpone, Pierre Tuffery, Philippe Derreumaux, P. Leslie Dutton, Colin Robinson, Andrew Hitchcock, C. Neil Hunter

(11) Identification of novel substrates of Shigella T3SA through analysis of its virulence plasmid-encoded secretomePLOS ONEOctober 26, 2017Laurie Pinaud, Mariana L. Ferrari, Robin Friedman, Nico Jehmlich, Martin von Bergen, Armelle Phalipon, Philippe J. Sansonetti, François-Xavier Campbell-Valoisand the following antibodies were used to detect proteins of interest: mouse anti-β-lactamase (Abcam ab12251 or Novus Biologicals NB120-12251; 1/ 1,000), rabbit anti-c-Myc (Santa Cruz sc789; 1/750), rabbit anti-RecA

(12) Injection of T3SS effectors not resulting in invasion is the main targeting mechanism of Shigella toward human lymphocytesProceedings of the National Academy of Sciences of the United States of AmericaSeptember 12, 2017Pinaud L, Samassa F, Porat Z, Ferrari ML, Belotserkovsky I, Parsot C, Sansonetti PJ, Campbell-Valois FX, Phalipon A.analyzed by SDS/PAGE, and proteins of interest were detected with mouse anti– β-lactamase antibody (Abcam, ab12251; or Novus Biologicals, NB120-12251; 1/1,000) and anti–mouse-HRP antibody (GE Healthcare; NXA931; 1/5,000), using

(13) A unifying mechanism for the biogenesis of membrane proteins co-operatively integrated by the Sec and Tat pathwayseLifeMay 17, 2017Fiona J Tooke, Marion Babot, Govind Chandra, Grant Buchanan, Tracy Palmergenerated by GenScript against the N-Terminal epitope CLPPHEPRVQDVDER), Bla (monoclonal antibody, Abcam ab12251), MBP (monoclonal antibody, NEB E8032L), BamA (polyclonal antibody, [Lehr et al., 2010]). Bands were

(14) The topology, structure and PE interaction of LITAF underpin a Charcot-Marie-Tooth disease type 1CBMC biologyDecember 7, 2016Ho AK, Wagstaff JL, Manna PT, Wartosch L, Qamar S, Garman EF, Freund SM, Roberts RC.AB_301364), mouse anti-GAPDH (Sigma, G8795, RRID: AB_1078991, Lot #092M4820V), mouse anti-β-lactamase (Abcam, ab12251, RRID: AB_298974), mouse anti-α-tubulin (DSHB, AA4.3, RRID: AB_579793, Lot #8/29/13), rabbit anti-GFP

(15) Rickettsia Sca4 Reduces Vinculin-Mediated Intercellular Tension to Promote SpreadCellOctober 20, 2016Lamason RL, Bastounis E, Kafai NM, Serrano R, Del Álamo JC, Theriot JA, Welch MD.2-mercaptoethanol) for 10 min and analyzed via Western blot using mouse anti-beta lactamase (Abcam ab12251) and mouse anti-OmpA 13-3 (kindly provided by Dr. Ted Hackstadt). Traction Force Microscopy Single

(16) Quantitative analysis of CagA type IV secretion by Helicobacter pylori reveals substrate recognition and translocation requirementsMolecular microbiologyApril 1, 2016Franziska Schindele, Evelyn Weiss, Rainer Haas, Wolfgang Fischer

(17) Multiple driving forces required for efficient secretion of autotransporter virulence proteinsThe Journal of biological chemistryApril 17, 2015Igor Drobnak, Esther Braselmann, Patricia L Clark

(18) Antibody-mediated immunity induced by engineeredEscherichia coliOMVs carrying heterologous antigens in their lumenJournal of Extracellular VesiclesAugust 11, 2014Laura Fantappiè, Micaela de Santis, Emiliano Chiarot, Filippo Carboni, Giuliano Bensi, Olivier Jousson, Immaculada Margarit, Guido Grandi

(19) Combinatorial Mutagenesis and Selection of Improved Signal Sequences and Their Application for High-Level Production of Translocated Heterologous Proteins in Escherichia coliApplied and Environmental MicrobiologyJanuary 15, 2013Tonje Marita Bjerkan Heggeset, Veronika Kucharova, Ingemar Naerdal, Svein Valla, Håvard Sletta, Trond Erling Ellingsen, Trygve Brautaset

(20) Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage InfectionPLoS ONENovember 20, 2012Jeanette E. Bröms, Lena Meyer, Kun Sun, Moa Lavander, Anders Sjöstedt

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Anti-Beta Lactamase antibody [8A5.A10] from Abcam

Abcam

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Anti-Beta Lactamase antibody [8A5.A10] from Abcam

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