Fig 1: Effects of ar-turmerone analogs on Nrf2-related proteins in LUHMES cells. (A) Representative immunoblot images of Nrf2, GCLM, GCLC, Keap1, HO-1, NQO1, and ß-actin in LUHMES cells under control conditions, treated with MPP+ (10 µM), and cotreated with MPP+ and ar-turmerone analogs (20 µM). Whole images of immunoblotting are presented in Figure S3. The arrow in the Keap1 blot indicates high-molecular-weight bands detected in cells treated with Atl and A2. (B) Quantitative analyses of immunoreactive bands of Nrf2, GCLM, GCLC, Keap1, HO-1, and NQO1. The band intensities were normalized with the immunoreactivity of ß-actin as an internal control. Data are presented as the mean ± SEM of three independent samples. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control, # p < 0.05, ### p < 0.001 (n = 3, one-way ANOVA, followed by a post hoc Tukey test).