Fig 2: HO-1 expression after 7i and 7l–p treatments for 48 h. (A–D) Representative immunoblot of HO-1 protein expression detected on cell homogenate of U87Mg, A172, A549, and DU145 cell lines treated with the selected compounds (10 µM). (A'–D') Bar graphs are representative of results from three independent experiments. Relative band density was quantified by using LI-COR software. Each protein level was expressed as a fold of change after normalization to actin used as a housekeeping protein. Data represent means ± SEM. *p < 0.05 vs control; #p < 0.05 vs control.

Fig 3: (A) Immunostaining for HO-1: note the positivity of a few inflammatory cells available mainly subepithelial in the skin of a patient positive for SARS-CoV-2 (Immunohistochemistry, Original Magnification: 40×). The light brown color is linked to “background noise” of the immunohistochemical reaction. (B) Photomicroghaph of skin biopsy of a patient negative for SARS-CoV-2 with higher expression of HO-1 in the epidermis and of inflammatory cells in subepithelial disposition (Immunohistochemistry, Original Magnification: 100×).

Fig 4: Correlations between HO-1 expression and clinicopathological features in patients with HBV-HCC who underwent hepatectomy.Notes: (A) Immunohistochemical staining of human HBV-HCC specimen with different intensity scores for HO-1 expression (low and high, respectively; ×400, scale bar =50 µm). (B) Kaplan–Meier plot of disease-free survival in HBV-HCC patients based on HO-1 expression levels. The high HO-1 expression group had favorable disease-free survival (P=0.003). HO-1 high expression is indicated by the green line. HO-1 low expression is indicated by the blue line.Abbreviations: HBV, hepatitis B virus; HCC, hepatocelluar carcinoma; HO-1, heme-oxygenase-1.

Fig 5: PPX administration accelerates the translocation of Nrf2 from the cytoplasm to the nucleus and increases the expression of HO-1 after a TBI. (A-C) Representative immunoblot intensity indicates cytosolic and nuclear Nrf2 (A,B), and HO-1 in the tissue lysate (C). The intensity of the targeted protein bands was normalized to ß-actin and histone H3. ***P<0.001, **P<0.01 and *P<0.05 compared with the sham group; #P<0.05 compared with the TBI group; ns, not significant (one-way ANOVA with Tukey's multiple comparisons test). Data are presented as mean±s.d. (n=4).