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Anti-Heparanase 1 antibody
Anti-Heparanase 1 antibody from Abcam
Be the first to write a review!
Citations:
(19)
Supplier Page
Supplier Page from
Abcam for
Anti-Heparanase 1 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Heparanase 1 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab85543
Antibody Type
Primary
Clonality
Polyclonal
Quantity
50 UG
Host
Rabbit
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Isotype
IgG
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Gene Name
HPSE
Reactivity
Chinese hamster, Human, Mouse
Target
Heparanase 1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (19)
(1)
Investigating the Role of Heparanase in Breast Cancer Development Utilising the MMTV-PyMT Murine Model of Mammary Carcinoma
Cancers (Basel)
June 5, 2023
Krishnath M. Jayatilleke, Hendrika M. Duivenvoorden, Gemma F. Ryan, Belinda S. Parker, Mark D. Hulett
h at room temperature.
Incubation
with the primary anti-HPSE antibody (2 μg/mL, rabbit polyclonal,
AB85543
,
Abcam
, Cambridge, UK) was performed at 4 °C for 16 h in 5% (w/v) skim milk in 0.1% (v/v) Tween-20/PBS.
(2)
Heparanase modulates the prognosis and development of BRAF V600E-mutant colorectal cancer by regulating AKT/p27Kip1/Cyclin E2 pathway
Oncogenesis
September 21, 2022
Liu M, Xu X, Peng K, Hou P, Yuan Y, Li S, Sun X, Shi Z, Zhang J, Dong Y,
et al
.
patients and xenografts was conducted using an automated system (BenchMark XT, Roche).
HPSE
antibody (
ab85543
,
Abcam
, Cambridge, UK) and p27Kip1 antibody (
25614-1-AP
,
Proteintech
, Wuhan, China) at a concentration
(3)
Heparanase promotes endothelial-to-mesenchymal transition in diabetic glomerular endothelial cells through mediating ERK signaling
Cell death discovery
February 16, 2022
Chang K, Xie Q, Niu J, Gu Y, Zhao Z, Li F, Qin Q, Liu X.
α-SMA: ab5694,
1:5000
, Cambridge, UK;
Snail1
/2:
ab180714
,
1:5000
,
Abcam
, Cambridge, UK;
HPSE
:
1:3000
,
ab85543
,
Abcam
, Cambridge, UK; phospho-ERK1/2 (p-ERK1/2):
4370
,
1:5000
,
Cell Signaling Technology
, Massachusetts,
(4)
Heparanase-Induced Activation of AKT Stabilizes β-Catenin and Modulates Wnt/β-Catenin Signaling during Herpes Simplex Virus 1 Infection
mBio
December 21, 2021
Koujah L, Madavaraju K, Agelidis AM, Patil CD, Shukla D.
E-cadherin (
Santa Cruz Biotechnology
; catalog no.
SC-8426
) at dilution
1:1,000
, HPSE (
Abcam
; catalog no.
ab85543
) at dilution
1:1,000
, and phospho-Akt (Ser473) (
CST
Signaling; catalog no.
9271
) at dilution
1:1,000
.
(5)
Upregulation of Fecal Epithelial Heparanase mRNA Is Associated with Increased Ulcerative Colitis Activity and Cancerization Risk
Digestive Diseases and Sciences
May 1, 2021
Ying Cui, Jun Zhang, Yan Chen, Le-gao Chen
(1,2-dimethylhydrazine, D0741) were purchased from MP Company in the USA.
Anti-heparanase 1
antibody (
ab85543
) and HRP labeled
goat
anti-rabbit II antibody (
ab205718
) were acquired from
Abcam
Company in the USA.
(6)
Heparanase from triple‑negative breast cancer and platelets acts as an enhancer of metastasis
International Journal of Oncology
August 27, 2020
Wen‑Jing Yang, Gan‑Lin Zhang, Ke‑Xin Cao, Xiao‑Ni Liu, Xiao‑Min Wang, Ming‑Wei Yu, Jin‑Ping Li, Guo‑Wang Yang
temperature for 1 h and incubated overnight with the following antibodies at 4°C: Hpa1 (
Abcam
, cat. no.
ab85543
,
1:2,000
), VEGF-a (
Abcam
, cat. no.
ab46154
,
1:1,000
),
hypoxia
-inducible factor HIF-1a (Immunoway, cat.
(7)
Heparanase potentiates the invasion and migration of pancreatic cancer cells via epithelial‑to‑mesenchymal transition through the Wnt/β‑catenin pathway
Oncology reports
August 1, 2020
Wang C, Wei Y, Wang G, Zhou Y, Zhang J, Xu K.
(TBST) for 2 h at room temperature and blotted with the following primary antibodies: HPSE (cat. no.
ab85543
,
1:500
,
Abcam
), E-cadherin (cat. no.
SAB4503751
,
1:500
,
Sigma-Aldrich
; Merck KGaA); vimentin (cat. no.
(8)
Induction of heparanase via IL-10 correlates with a high infiltration of CD163+ M2-type tumor-associated macrophages in inflammatory breast carcinomas
Matrix Biology Plus
May 1, 2020
Mennatullah El-Nadi, Hebatallah Hassan, Moshira Ezzat Saleh, Eyyad Nassar, Yahia Mahmoud Ismail, Mahmoud Amer, Burkhard Greve, Martin Götte, Mohamed El-Shinawi, Sherif Abdelaziz Ibrahim
milk/TBST solution, washed thrice with TBST, and probed with anti-
human
heparanase antibody (
1:500
;
ab85543
,
Abcam
, UK) overnight at 4C°. Following 1 h
incubation
at room temperature with HRP-conjugated secondary
(9)
Crocin alleviates lipopolysaccharide-induced acute respiratory distress syndrome by protecting against glycocalyx damage and suppressing inflammatory signaling pathways
Inflammation Research
March 1, 2020
Dong Zhang, Bo-yang Qi, Wei- wei Zhu, Xiao Huang, Xiao-zhi Wang
(ab170099), p-ERK (ab201015), p-JNK (ab4821), p-p38 (ab47363), LaminB1 (
ab133741
), MMP-9 (
ab38898
), HPA (
ab85543
), SDC-4 (
ab24511
), Ly6g (
ab25377
), β-actin (
ab8224
), GAPDH (
ab181602
),
MMP-9 inhibitor
(MMP-9 inhib,
(10)
Heparanase-Dependent Remodeling of Initial Lymphatic Glycocalyx Regulates Tissue-Fluid Drainage During Acute Inflammation in vivo
Frontiers in Immunology
October 4, 2019
Samantha Arokiasamy, Ross King, Hidayah Boulaghrasse, Robin N. Poston, Sussan Nourshargh, Wen Wang, Mathieu-Benoit Voisin
(polyclonal
ab53842
,
Abcam
, Cambridge, UK) and rabbit anti-
mouse
/
human
heparanase I Ab (polyclonal
ab85543
,
Abcam
). Isolectin-B4 (IB4), Maackia amurensis Lectin-1 (MAL-1),
Sambucus nigra
Agglutinin (SNA) and
(11)
Syndecans and Enzymes for Heparan Sulfate Biosynthesis and Modification Differentially Correlate With Presence of Inflammatory Infiltrate in Periodontitis
Frontiers in Physiology
September 25, 2019
Roko Duplancic, Marija Roguljic, Ivan Puhar, Nika Vecek, Ruzica Dragun, Katarina Vukojevic, Mirna Saraga-Babic, Darko Kero
polyclonal
anti-NDST2
(
1:100
, ab1511141,
Abcam
, United Kingdom), rabbit polyclonal anti-HPSE1 (
1:200
,
ab85543
,
Abcam
, United Kingdom) and
mouse
monoclonal anti-CD45 [MEM-28] (
1:200
,
ab8216
,
Abcam
, United Kingdom)
(12)
Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase
Molecular Medicine Reports
June 27, 2019
Xiangjun Zhou, Mengmou Hu, Zhenghui Ge
(cat. no.
ab7481
,
Abcam
) for 2 h at room temperature. Primary antibody against HPSE (
1:200
; cat. no.
ab85543
;
Abcam
) was then applied at 4°C overnight. Following washing, the sections were incubated with peroxidase-conjugated
(13)
Syndecans and Enzymes Involved in Heparan Sulfate Biosynthesis and Degradation Are Differentially Expressed During Human Odontogenesis
Frontiers in Physiology
June 14, 2018
Darko Kero, Tanja Simic Bilandzija, Lidija Lasic Arapovic, Katarina Vukojevic, Mirna Saraga-Babic
polyclonal
anti-NDST2
(
1:100
,
ab151141
,
Abcam
, United Kingdom), and rabbit polyclonal anti-HPSE1 (
1:200
,
ab85543
,
Abcam
, United Kingdom). Secondary antibodies were used at
1:400
dilution – anti-
mouse
Alexa Fluor 488
(14)
Upregulation of Endothelin-1/Endothelin A Receptor Expression Correlates with Heparanase Expression in Ovarian Carcinoma
Iran J Med Sci
June 13, 2018
Nungki Anggorowati, Ahmad Ghozali, Irianiwati Widodo, Dwi Cahyani Ratna Sari, Muhammad Mansyur Romi, Nur Arfian
patients.
Immunohistochemistry
The paraffin-embedded tissues were used
for immunohistochemistry
with heparanase (
AB85543
,
Abcam
, dilution
1:200
), ETAR (
SC33535
,
Santa Cruz
, dilution
1:100
), and Ki-67 (CRM 325 B, Biocare Medical,
(15)
Hyperoside pre-treatment prevents glomerular basement membrane damage in diabetic nephropathy by inhibiting podocyte heparanase expression
Scientific Reports
December 1, 2017
Xiaofei An, Lin Zhang, Yanggang Yuan, Bin Wang, Qiuming Yao, Ling Li, Jisheng Zhang, Ming He, Jinan Zhang
UK) in the dilution of
1:100
at 4 °C overnight. The rabbit anti-
mouse
heparanase primary antibody (
Ab85543
,
Abcam
, Cambridge, MA) which recognizes 65 kD precursor as well as the 50 kD and 8 kD subunits of heparanase
(16)
NK cell heparanase controls tumor invasion and immune surveillance
The Journal of clinical investigation
June 30, 2017
Putz EM, Mayfosh AJ, Kos K, Barkauskas DS, Nakamura K, Town L, Goodall KJ, Yee DY, Poon IK, Baschuk N,
et al
.
skim milk powder before the addition of one of the following primary antibodies: anti-HPSE (
Abcam
; catalog
ab85543
; diluted
1:1,000
or Insight Biotechnology; catalog TA332866; diluted
1:4,000
) or anti–β-actin (
Cell Signaling Technology
(17)
Elemene inhibits the migration and invasion of 4T1 murine breast cancer cells via heparanase
Molecular Medicine Reports
May 26, 2017
Yi Zhang et al.
purchased from Aventis Intercontinental (Paris, France). Primary antibodies against heparanase (cat no.
ab85543
) and VEGF (cat no.
ab46154
) were purchased from
Abcam
(Cambridge, UK). The primary antibodies against
(18)
Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways
Molecules
May 15, 2017
Yi Zhang et al.
Reagents Fucoidan was purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibodies against heparanase (
ab85543
), VEGF (
ab46154
), and CD31 (
ab28364
) were purchased from
Abcam
Biotechnology (Cambridge, UK). The primary
(19)
Expression of heparanase is associated with breed-specific morphological characters of placental folded bilayer between Yorkshire and Meishan pigs
Biology of reproduction
March 1, 2014
Linjun Hong, Chunyan Hou, Xiaoping Li, Changchun Li, Shuhong Zhao, Mei Yu
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Anti-Heparanase 1 antibody from Abcam
Supplier Page
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Product Specs
Company Info
Citations
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