Fig 1: NK cells in the livers of obese mice acquire an ILC1-like transcriptional profile. (A) NK cells and ILC1 were sorted from the livers of lean and obese mice and examined by RNASeq. Genes that were differentially expressed by >2-fold, with padj <0.05, in NK cells of lean vs. obese mice are shown. Genes that are also differentially expressed in ILC1 compared to NK (>2-fold, with padj <0.05) are marked with an asterisk. (B,C) Protein expression of the inhibitory receptors CD200R1 (B) and LAG3 (C) in NK cells and ILC1 of lean (blue) compared to obese (red) mice. Gray traces represent FMO controls. (D) Expression of Eomes in NK cells and ILC1 of lean (blue) compared to obese (red) mice. Gray traces represent internal negative controls. (E) Expression of CD49a in Lineage-negative NK1.1+ CD49b+ cells (NK) and Lineage-negative NK1.1+ CD49b- cells (ILC1) of lean (blue) compared to obese (red) mice. Gray traces represent FMO controls. n = 6 mice per group; significance was determined using Mann Whitney U-Tests; medians and IQRs are shown.