Fig 1: CUX1 and ITSN1 are target genes of miR-193a-5p and associated with poor prognosis of colorectal cancer (CRC). (A) The protein expression analysis of CUX1 and ITSN1 in CRC cells after transfection with miR-193a-5p mimics or miR-NC. (B) The mRNA expression analysis of CUX1 and ITSN1 in HCT-8 and SW480 cells after transfection with miR-193a-5p mimics or miR-NC (*p < 0.05, **p < 0.01, ***p < 0.001). (C,D) Dual-luciferase reporter assay. Reporter constructs containing either wild-type CUX1/ITSN1 3'-UTR or CUX1/ITSN1 3'-UTR with mutation at the predicted miR-193a-5p target sequence were transfected into CRC cells, along with miR-193a-5p mimics or miR-NC; the relative luciferase activity was assayed (*p < 0.05, **p < 0.01, ***p < 0.001). (E,F) The overall survival (OS) of CRC patients stratified by CUX1 and ITSN1 expression levels.