Western blot analysis of Rbp4 in Hepa 1‚6 mouse hepatoma cell line. Samples were incubated in Rbp4 polyclonal antibody (Product # PA5-47495) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for RBP4/Retinol‚Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions.