Fig 2: Clinical validation of nCD64 for sepsis stratification and prognostication.(a) The CD64 expression histograms for monocytes, neutrophils and lymphocytes for n=450 blood samples. The inset histogram represents nCD64 expression for all the blood samples. (b) The plot represents the ratio of mCD64/ nCD64 versus nCD64. (c) ROC curves showing sepsis predictability using Quick SIRS (AUC=0.7) and SIRS + CD64 (AUC=0.77). (d) The box plots showing the control nCD64 expression value from 316 blood samples collected from 68 patients (who recovered later) at different time windows of their hospital stay. (e) The box plots showing the control nCD64 expression value from 94 blood samples collected from six patients who are at different times of their hospital stay. Unfortunately, these patients did not recover. (f) The nCD64 and WBC counts are used for sepsis prognosis for all the six time windows using ANN model. ROC curves for TW1 (left) and TW5 (right) show the highest AUC>0.9 for sepsis prognosis by predicting the recovery of the patients. P-values are reported in Methods section.

Fig 3: Overview of the electrical cell-counting technology.(a) Process schematic of the differential expression-based cell-counting technology. Whole blood (10 µl) is infused in the biochip along with lysing and quenching buffers, to preferentially lyse erythrocytes. Cells are electrically counted and differentiated based on their size using microfabricated electrodes. Anti-CD64 (clone 10.1) antibody is initially adsorbed in the chamber. The CD64+ cells get captured based on their CD64 expression level. The difference in the cell counts from cell counters is linearly correlated with the nCD64 expression level. (b) The resulting pulse amplitude histogram representing lymphocytes and granulocytes + monocytes as two distinct populations. (c) Correlation (coefficient of determination: R2=0.89, P<0.0001) in between biochip total leukocytes versus control leukocyte counts obtained from haematology analyzer using n=181 blood samples. (d) The correlation (coefficient of determination: R2=0.88, P<0.0001) in between biochip total granulocytes + monocytes versus control cell counts obtained from haematology analyzer.