APC/Cyanine7 anti-mouse CD3 100221 from BioLegend

Product Specs
ItemAPC/Cyanine7 anti-mouse CD3
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100221
NCBI Gene AliasesCtg-3, Ctg3, T3g
HostRat
Quantity25 µg
ReactivityMouse
ApplicationsFlow Cytometry (FC)
Clone17A2
Conjugate/TagAPC_CYANINE7
IsotypeIgG2b, Kappa
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
ClonalityMonoclonal
TargetCD3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axisScience advancesApril 14, 2023Tong L, Zhang S, Liu Q, Huang C, Hao H, Tan MS, Yu X, Lou CKL, Huang R, Zhang Z, et al.min at 4°C. Cells were stained for various surface markers [CD4 (BioLegend, 100432), CD3 (BioLegend, 100222), CD8 (BioLegend, 100759), CD45 (BD Bioscience, 562420), CD25 (BioLegend, 102008), CD11c (Invitrogen,

(2) Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and EosinophilsAdvanced ScienceMarch 1, 2023Yuanyuan Zhang, Tianxiao Liu, Zhiyong Deng, Wenqian Fang, Xian Zhang, Shuya Zhang, Minjie Wang, Songyuan Luo, Zhaojie Meng, Jing Liu, et al.directly for antibody staining. For cell surface staining, single cells were incubated with CD3 (1:200, 100222, BioLegend) and CD4 (1:200, 11‐0042‐85, eBioscience) antibodies for Th1, Th2, Th17, and Treg analysis

(3) Biometabolites of Citrus unshiu Peel Enhance Intestinal Permeability and Alter Gut Commensal BacteriaNutrientsJanuary 9, 2023Lee SH, Seo D, Lee KH, Park SJ, Park S, Kim H, Kim T, Joo IH, Park JM, Kang YH, et al.Cell Signaling Technology). The antibodies used for the flow cytometry were CD3 (clone 17A2, catalog no. 100222; BioLegend, San Diego, CA, USA), CD4 (clone GK1.5, catalog no. 48-0041-82; eBioscience, San Diego, CA,

(4) Precision cancer sono-immunotherapy using deep-tissue activatable semiconducting polymer immunomodulatory nanoparticlesNature CommunicationsDecember 1, 2022Jingchao Li, Yu Luo, Ziling Zeng, Dong Cui, Jiaguo Huang, Chenjie Xu, Liping Li, Kanyi Pu, Ruiping Zhangclone 16-10A1), CD86-APC (Biolegend, cat. no. 105114, clone PO3), CD3-APC/Cyanine7 (Biolegend, cat. no. 100222, clone 17A2), CD4-FITC (Biolegend, cat. no. 100406, clone GK1.5), CD8-PE (Biolegend, cat. no. 140408,

(5) Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic TransformationCancer discoveryOctober 5, 2022Chen S, Vedula RS, Cuevas-Navarro A, Lu B, Hogg SJ, Wang E, Benbarche S, Knorr K, Kim WJ, Stanley RF, et al.45-0452-82, 1:200), anti–B220-BV605 (RA3-6B2, BioLegend, 103244, 1:200), anti–CD3-APC-Cy7 (17A2, BioLegend, 100222, 1:200), anti–CD3-PE-Cy7 (17A2, BioLegend, 100220, 1:200), anti–CD3-APC-Cy7 (17A2, BioLegend, 100222,

(6) A Novel Selective Axl/Mer/CSF1R Kinase Inhibitor as a Cancer Immunotherapeutic Agent Targeting Both Immune and Tumor Cells in the Tumor MicroenvironmentCancers (Basel)October 2, 2022Yeejin Jeon, Hwankyu Kang, Yeongin Yang, Dongsik Park, Baejung Choi, Jeongjun Kim, Jaeseung Kim, Kiyean NamThe cells were stained with CD45-BV750 (BioLegend; #103157, clone 30-F11), CD3-APC-Cy7 (BioLegend; #100222, clone 17A2), CD4-Alexa Fluor 700 (BioLegend; #100430, clone GK1.5), and CD8a-Pacific Orange (Thermo Fisher Scientific

(7) Classical and γδ T cells are each independently sufficient to establish protection against a classical strain of Klebsiella pneumoniaeFrontiers in Cellular and Infection MicrobiologyAugust 31, 2022Joseph J. Mackel, Catherine Morffy Smith, Rachel K. Wasbotten, Joy Twentyman, David A. Rosen(BD Biosciences 553142) for 10 min, and stained at 4°C for 30 min with CD45-BV510 (103138), CD3-APC/Cyanine7 (100222), CD4-FITC (116004), TCRγ/δ-PE (118108), CD69-BV421 (104545), from Biolegend, and CD11b (557960), Ly6G-FITC

(8) Neutrophils incite and macrophages avert electrical storm after myocardial infarctionNature Cardiovascular ResearchAugust 29, 2022Jana Grune et al.101212, BioLegend), CD45-BV711 (30-F11, 1:600, 103147, BioLegend), CD3-APC/Cy7 (clone 17A2, 1:200, 100221, BioLegend), CD19-APC/Cy7 (clone 6D5, 1:300, 115529, BioLegend), B220-APC/Cy7 (clone RA3–6B2, 1:300,

(9) Delivering an mRNA vaccine using a lymphatic drug delivery device improves humoral and cellular immunity against SARS-CoV-2Journal of molecular cell biologyAugust 26, 2022Chen R, Xie H, Khorsandzadeh S, Smith M, Shaabani N, Hu Q, Lyu X, Wang H, Lim WL, Sun H, et al.Biolegend), BV421 anti-mouse IL-4 (Cat: 504127, Biolegend), allophycocyanin-Cy7 anti-mouse CD3 (Cat: 100222, Biolegend), PE-Cy7 anti-mouse CD4 (Cat: 25-0041-82, Invitrogen), and allophycocyanin anti-mouse CD8a

(10) Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain traumaNature communicationsAugust 12, 2022Zhang S, Li Y, Sun S, Liu L, Mu X, Liu S, Jiao M, Chen X, Chen K, Ma H, et al.(Biolegend, 104714), FITC anti-mouse CD206 (MMR) (Biolegend, 141704), APC/Cyanine7 anti-mouse CD3 (Biolegend, 100222), PE/Cyanine7 anti-mouse CD8a (Biolegend, 100722), FITC anti-mouse CD4 (Biolegend, 100406), PE anti-mouse NK-1.1

(11) Olive Pomace Phenolic Compounds: From an Agro-Industrial By-Product to a Promising Ocular Surface Protection for Dry Eye DiseaseJournal of Clinical MedicineAugust 11, 2022Nikolaos Katsinas, Uta Gehlsen, Laura García-Posadas, Soraya Rodríguez-Rojo, Philipp Steven, María J. González-García, Amalia Enríquez-de-Salamanca(mg/mL) Anti-mouse CD3 (17A2),CD3+ T cells,allophycocyanin-cyanine 7 (APC-Cy7),"Biolegend (San Diego, CA, USA)",100221,0.10 "Biolegend (San Diego, CA, USA)",Anti-mouse CD4 (GK1.5),CD4+ T cells,fluorescein isothiocyanate

(12) Cell-penetrating peptides enhance peptide vaccine accumulation and persistence in lymph nodes to drive immunogenicityProceedings of the National Academy of Sciences of the United States of AmericaAugust 9, 2022Backlund CM, Holden RL, Moynihan KD, Garafola D, Farquhar C, Mehta NK, Maiorino L, Pham S, Iorgulescu JB, Reardon DA, et al.for Flow Cytometry. CD8a (BioLegend #100712), IFN-γ (BioLegend #505808), TNF-α (BioLegend #506313), CD3e (BioLegend #100222), CD19 (BioLegend #115529), F4/80 (BD Biosciences #565614), CD11b (BioLegend #101243), CD11c (BioLegend

(13) Targeting LIPA independent of its lipase activity is a therapeutic strategy in solid tumors via induction of endoplasmic reticulum stressNature cancerJuly 1, 2022Liu X, Viswanadhapalli S, Kumar S, Lee TK, Moore A, Ma S, Chen L, Hsieh M, Li M, Sareddy GR, et al.and fluorophore-labeled mAbs to surface markers, including CD3 (APC-Cy7, Clone 17A2; BioLegend, no. 100221), IgD (FITC, Clone 11-26 c.2a; BioLegend, no. 405704), CD138 (PE-Cy7, Clone 281-2; BioLegend, no. 142514),

(14) A long-acting interleukin-7, rhIL-7-hyFc, enhances CAR T cell expansion, persistence, and anti-tumor activityNature communicationsJune 13, 2022Kim MY, Jayasinghe R, Devenport JM, Ritchey JK, Rettig MP, O'Neal J, Staser KW, Kennerly KM, Carter AJ, Gao F, et al.stained with LIVE/DEAD fixable yellow (Thermo Fisher Scientific, L34967) and CD3-APC/Cy7 (BioLegend, 100221), then treated with FIX & PERM cell permeabilization kit (ThermoFisher Scientific, GAS003) with the

(15) EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis modelThe Journal of experimental medicineMay 2, 2022Tao X, Zhang R, Du R, Yu T, Yang H, Li J, Wang Y, Liu Q, Zuo S, Wang X, et al.primary antibodies. The primary antibodies were diluted as follows: anti-CD3-allophycocyanin (APC)-cy7 (#100222, 1:200; BioLegend), anti-NK1.1-BV421 (#108732, 1:200; BioLegend), anti-CD11b-FITC (#101206, 1:200; BioLegend),

(16) Aedes aegypti sialokinin facilitates mosquito blood feeding and modulates host immunity and vascular biologyCell ReportsApril 12, 2022Ines Martin-Martin et al.Antibody,BioLegend,"Cat# 105825; RRID: AB_1626278, clone 2B8" APC/Cyanine7 anti-mouse CD3 Antibody,BioLegend,"Cat# 100222; RRID: AB_2057374, clone 17A2" FITC anti-mouse/human CD11b Antibody,BioLegend,"Cat# 101206; RRID: AB_312789,

(17) In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapyScience AdvancesJanuary 21, 2022Jeong Pil Han et al.(BioLegend, San Diego, CA, USA, 100706), fluorescein isothiocyanate (FITC) anti-mouse CD8 (BioLegend, 100222), and phycoerythrin (PE) anti-mouse IFN-γ (BioLegend, 505808). Measurement of anti-Cas9 antibody response The

(18) A role for whey acidic protein four-disulfide-core 12 (WFDC12) in the pathogenesis and development of psoriasis diseaseFrontiers in immunologyJanuary 1, 2022Zhao F, Zhang C, Li G, Zheng H, Gu L, Zhou H, Xiao Y, Wang Z, Yu J, Hu Y, et al.Lakes, NJ, USA, 564996) for 10 min. Next, stain cells with following surface antibodies: APC/Cy7-CD3 (100222, BioLegend, San Diego, CA, USA), PerCP/Cy5.5-CD4 (100434, BioLegend, San Diego, CA, USA), PE/Cy7-CD8

(19) Identification of heterogeneous subsets of aortic interleukin-17A-expressing CD4(+) T cells in atherosclerotic miceInternational journal of immunopathology and pharmacologyJanuary 1, 2022Lin G, Zhang L, Yan Z, Jiang W, Wu B, Li D, Xiong X.Antibody,Cat #,Clone #,Company APC/Cyanine7 anti-mouse CD3,100221,17A2,BioLegend Pacific blue anti-mouse CD4,100427,GK1.5,BioLegend PE/Cyanine7 anti-mouse CD4,100421,GK1.5,BioLegend PE anti-mouse CD8a

(20) Age-Associated Changes to Lymph Node Fibroblastic Reticular CellsFront AgingJanuary 1, 2022Kwok T, Medovich SC, Silva-Junior IA, Brown EM, Haug JC, Barrios MR, Morris KA, Lancaster JN.fluorochrome-conjugated antibodies directed against the following mouse targets: CD3-APCCy7 (17A2, Biolegend 100222), CD4-BV510 (RM4-5, Biolegend 100553), CD8-BUV395 (53-6.7, BD Biosciences 563786), CD44-AF700 (IM7,

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(1)

2/6/2023Good Anti-Mouse CD3 APC-Cy7Dr. Andreia LinoAntibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in ... Read Review
(1)

7/26/2016Staining Mouse WBC With Anti-CD3 APC/Cy7 Adam GuessThis antibody was used as a T cells marker in a hematopoietic lineage panel. It was chosen because it is a commonly used clone, APC/Cy7 gives enough separation to fully resolve T cell populations, and... Read Review

APC/Cyanine7 anti-mouse CD3 from BioLegend

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APC/Cyanine7 anti-mouse CD3 from BioLegend

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