Fig 2: The expression of signature genes in OS and the effects of GALNT14 in OS. (A–D) The mRNA expression of GALNT14, ISLR, ITTGB5, and IFI44 was examined by RT-qPCR in hFOB 1.19 and osteosarcoma cell lines. (E) Kaplan–Meier curve of the expression level of GALNT14 on OS. (F) The protein expression of GALNT14 was measured by western blot in hFOB 1.19 and osteosarcoma cell lines. (G) The protein level of GALNT14 was quantified by the Image J software. (H) Knockdown of GALNT14 with siRNA in 143B cells was confirmed by RT-qPCR. (I) Knockdown of GALNT14 with siRNA in 143B cells was confirmed by western blot. (J) The protein level of GALNT14 was quantified by the Image J software. (K) Growth curves of cells were examined by CCK-8 assays after the knockdown of GALNT14 in 143B cells. (L) Colony formation assay was detected after the knockdown of GALNT14 in 143B cells. (M) Knockdown of GALNT14 inhibited proliferation of osteosarcoma cells 143B according to colony formation assay. ns, no significance,*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.