PE/Cyanine7 anti-mouse CD3 100220 from BioLegend

Product Specs
ItemPE/Cyanine7 anti-mouse CD3
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100220
NCBI Gene AliasesCtg-3, Ctg3, T3g
HostRat
Quantity100 µg
Clone17A2
ReactivityMouse
ApplicationsFlow Cytometry (FC)
IsotypeIgG2b, Kappa
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
ClonalityMonoclonal
TargetCD3
Conjugate/TagPE_CYANINE7

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse modelNature communicationsJuly 4, 2023Hu M, Li T, Ma X, Liu S, Li C, Huang Z, Lin Y, Wu R, Wang S, Lu D, et al.anti mouse CD3 (Biolegend 100204, clone: 17A2, 1:400), APC/CY7 conjugated anti mouse CD3 (Biolegend 100220, clone: 17A2, 1:400), PE conjugated anti mouse CD19 (Biolegend 152408, clone: 1D3/CD19, 1:400), PE/CY7

(2) Histone H2A Lys130 acetylation epigenetically regulates androgen production in prostate cancerNature CommunicationsJune 9, 2023Thanh Nguyen, Dhivya Sridaran, Surbhi Chouhan, Cody Weimholt, Audrey Wilson, Jingqin Luo, Tiandao Li, John Koomen, Bin Fang, Nagireddy Putluri, et al.Fixable Aqua Dead Cell stain (Thermo Fisher Scientific, Cat#L34957, 1:400) anti-CD3 PECy7 (BioLegend, Cat#100220, Clone 17A2, 1:400), anti-CD8 APC (BioLegend, Cat#553035, Clone 53-6.7, 1:400), anti-PD1 FITC (BioLegend,

(3) The T-cell-directed vaccine BNT162b4 encoding conserved non-spike antigens protects animals from severe SARS-CoV-2 infectionCellMay 25, 2023Arieta CM, Xie YJ, Rothenberg DA, Diao H, Harjanto D, Meda S, Marquart K, Koenitzer B, Sciuto TE, Lobo A, et al.for flow cytometry staining using the following antibodies from Biolegend: CD45.2 BV421 - 109832, CD3 PE-Cy7 - 100220, CD19 BV605 - 115540, CD4 BV510 - 100559, CD8 AF488 - 100723, CD62L PE - 104408, CD44 BV711 - 103057,

(4) Targeting PD-L2-RGMb overcomes microbiome-related immunotherapy resistanceNatureMay 1, 2023Park JS, Gazzaniga FS, Wu M, Luthens AK, Gillis J, Zheng W, LaFleur MW, Johnson SB, Morad G, Park EM, et al.anti-mouse CD3ε clone 17A2 (BioLegend Cat#100225 1:200), PE-Cy7 anti-mouse CD3ε clone 17A2 (BioLegend Cat#100220 1:200), Brilliant Violet 570 anti-mouse TCRβ clone H57-597 (BioLegend Cat#109231 1:200), BUV615 anti-mouse

(5) American ginseng with different processing methods ameliorate immunosuppression induced by cyclophosphamide in mice via the MAPK signaling pathwaysFrontiers in ImmunologyApril 21, 2023Yan-Ting Zhang, Wei Tian, Yu-Shun Lu, Zhi-Man Li, Duo-Duo Ren, Yue Zhang, Ji-Yue Sha, Xiao-Hui Huo, Shan-Shan Li, Yin-Shi Sunwere purchased from Renhe Tang Pharmaceutical Co. Ltd. (Linyi, China). PE/Cyanine7 anti-mouse CD3 (100220), FITC anti-mouse CD4 (100509), and APC anti-mouse CD8a (100712) were obtained from BioLegend (San Diego,

(6) Multi-omic analysis of the cardiac cellulome defines a vascular contribution to cardiac diastolic dysfunction in obese female miceBasic research in cardiologyMarch 29, 2023Dona MSI, Hsu I, Meuth AI, Brown SM, Bailey CA, Aragonez CG, Russell JJ, Krstevski C, Aroor AR, Chandrasekar B, et al.LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (1:40, Invitrogen #L34957), CD3-PE-Cy7 (1:100; Biolegend #100220), CD4-PE (1:100; BD Biosciences #5530449), CD11b-FITC (1:200; Biolegend #101206), CD68-PE (1:50; Biolegend

(7) Chimeric mRNA based COVID-19 vaccine induces protective immunity against Omicron and Delta variantsMol Ther Nucleic AcidsDecember 13, 2022Qidong Hu, Ying Zhao, Namir Shaabani, Xiaoxuan Lyu, Colin Powers, Haotian Sun, Vincent Cruz, Karen Stegman, Jia Xu, Amber Fossier, et al.biotinylated SARS-CoV-2-S1 protein (BioLegend 793804), anti-biotin (BioLegend 409003), CD3 (BioLegend 100220), CD3 (BioLegend 100204), CD4 (BioLegend 100540), ICOS (BioLegend 117420), CD8 (BioLegend 557654), CD45

(8) Enhancement of antitumor immunotherapy using mitochondria-targeted cancer cell membrane-biomimetic MOF-mediated sonodynamic therapy and checkpoint blockade immunotherapyJournal of NanobiotechnologyDecember 1, 2022Jiali Luo, Xue Wang, Zhan Shi, Yiqing Zeng, Liangcan He, Jing Cao, Yu Sun, Tao Zhang, Pintong HuangBiolegend, UK), Brilliant Violet 605™ anti-mouse CD45 (103139, Biolegend, UK), PE/Cyanine7 anti-mouse CD3 (100220, Biolegend, UK), Brilliant Violet 421™ anti-mouse CD4 (100438, Biolegend, UK), Alexa Fluor® 647 anti-mouse

(9) Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancerCell reportsNovember 8, 2022Millar FR, Pennycuick A, Muir M, Quintanilla A, Hari P, Freyer E, Gautier P, Meynert A, Grimes G, Coll CS, et al.(Brilliant Violet 421),Biolegend,Cat #103133; RRID:AB_10899570 Anti-mouse CD3 (PE/Cyanine7),Biolegend,Cat #100219; RRID:AB_1732068 Anti-mouse CD4 (PE),Biolegend,Cat #100407; RRID:AB_312692 Anti-mouse CD8 (Super bright

(10) Identification of environmental factors that promote intestinal inflammationNatureNovember 1, 2022Sanmarco LM, Chao CC, Wang YC, Kenison JE, Li Z, Rone JM, Rejano-Gordillo CM, Polonio CM, Gutierrez-Vazquez C, Piester G, et al.Thermo Fisher Scientific), PECy7 or PE-Dazzle594 anti-mouse-CD11c (N418, BioLegend) or PECy7-anti mouse CD3 (100220 and 117348, BioLegend) antibodies. After 30 min on ice, cells were washed and sorted on the FACS Aria

(11) Mouse phospholipid phosphatase 6 regulates dendritic cell cholesterol, macropinocytosis, and allergen sensitizationiScienceOctober 21, 2022Thayse R. BrÃ¼ggemann, Troy Carlo, Nandini Krishnamoorthy, Melody G. Duvall, Raja-Elie E. Abdulnour, Julie Nijmeh, Hong Yong Peh, Harilaos Filippakis, Roxanne H. Croze, Byoungsook Goh, et al.AB_493343 anti-mouse CD40,Biolegend,clone 3/23; cat#124609; RRID: AB_1134084 anti-mouse CD3,Biolegend,clone 17A2; cat#100219; RRID: AB_1732068 anti-mouse CD4,Biolegend,clone RM4-5; cat#100537; RRID: AB_893331 anti-mouse CD19,Biolegend,clone

(12) Innate type 2 immunity controls hair follicle commensalism by Demodex mitesImmunityOctober 11, 2022Ricardo-Gonzalez RR, Kotas ME, O'Leary CE, Singh K, Damsky W, Liao C, Arouge E, Tenvooren I, Marquez DM, Schroeder AW, et al.μg",BioLegend,Cat#134314; RRID:AB_10613298 "PE/Cy7 anti-mouse CD3 Antibody, 100 μg",BioLegend,Cat#100220; RRID:AB_1732057 "Pacific Blue™ anti-mouse F4/80 Antibody, 100 μg",BioLegend,Cat#123124; RRID:AB_893475 "Brilliant

(13) Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic TransformationCancer discoveryOctober 5, 2022Chen S, Vedula RS, Cuevas-Navarro A, Lu B, Hogg SJ, Wang E, Benbarche S, Knorr K, Kim WJ, Stanley RF, et al.103244, 1:200), anti–CD3-APC-Cy7 (17A2, BioLegend, 100222, 1:200), anti–CD3-PE-Cy7 (17A2, BioLegend, 100220, 1:200), anti–CD3-APC-Cy7 (17A2, BioLegend, 100222, 1:200), anti–Gr-1-APC (RB6-8C5, eBioscience, 25-5931-82,

(14) Japanese Kampo Medicine Juzentaihoto Improves Antiviral Cellular Immunity in Tumour-Bearing HostsEvidence-Based Complementary and Alternative MedicineAugust 13, 2022Shun Takaku, Masumi Shimizu, Rimpei MoritaBioLegend): phycoerythrin (PE)/cyanine dye (Cy)7-conjugated anti-CD3 (clone 17A2, catalogue number: 100220), fluorescein isothiocyanate (FITC)-conjugated anti-CD4 (clone GK1.5, catalogue number: 100405), FITC-conjugated

(15) Localization of Salmonella and albumin-IL-2 to the tumor microenvironment augments anticancer T cell immunityJournal of Biomedical ScienceAugust 12, 2022Yu-Jui Kung, Brandon Lam, Ssu-Hsueh Tseng, Alana MacDonald, Hsin-Fang Tu, Suyang Wang, John Lin, Ya Chea Tsai, T. C. Wu, Chien-Fu HungTetramer-PE,TB-5001-M,MBL CD8a-FITC,100706,Biolegend IFNγ-APC,505809,Biolegend TNFα-BV650,506333,Biolegend CD4-BV785,100453,Biolegend IL2-PE,503807,Biolegend CD3mc-PE-Cy7,100219,Biolegend CD45-Alexa Fluor 700,147715,Biolegend Antibodies

(16) Omicron Booster in Ancestral Strain Vaccinated Mice Augments Protective Immunities Against Both Delta and Omicron VariantsFrontiers in ImmunologyJuly 6, 2022Liqiu Jia, Yang Zhou, Shaoshuai Li, Yifan Zhang, Dongmei Yan, Wanhai Wang, Wenhong Zhang, Yanmin Wan, Chao QiuPharmingen) for 15 min at room temperature, surface markers (PE/Cyanine7-labeled anti-mouse CD3, Cat# 100220, BioLegend; APC-labeled anti-mouse CD4, Cat# 100412, BioLegend; PE-labeled anti-mouse CD8, Cat# 100708,

(17) Poroptosis: A form of cell death depending on plasma membrane nanopores formationiScienceJune 17, 2022Hao Li, Zihao Wang, Xiaocui Fang, Wenfeng Zeng, Yanlian Yang, Lingtao Jin, Xiuli Wei, Yan Qin, Chen Wang, Wei LiangAB_466088 Anti-mouse CD3 APC,Biolegend,Cat # 100236; RRID: AB_2561456 Anti-mouse CD3 PE-CY7,Biolegend,Cat # 100220; RRID: AB_1732057 Anti-mouse CD11c APC,Biolegend,Cat # 117310; RRID:AB_313779 Anti-mouse CD4 Brilliant

(18) Intra‐articular injection of triamcinolone acetonide sustains macrophage levels and aggravates osteophytosis during degenerative joint disease in miceBritish Journal of PharmacologyJune 1, 2022Mauricio N. Ferrao Blanco, Yvonne M. Bastiaansen Jenniskens, Nicole Kops, Athina Chavli, Roberto Narcisi, Sander M. Botter, Pieter J. M. Leenen, Gerjo J. V. M. van Osch, Niamh FahyRRID:AB_313099) to identify myeloid cells and specific monocyte subsets, as well as CD3 (BioLegend Cat# 100220, RRID:AB_1732057), NK1.1 (BioLegend Cat# 108713, RRID:AB_389363), CD19 (BioLegend Cat# 115520, RRID:AB_313655),

(19) TH17 cells promote CNS inflammation by sensing danger signals via MincleNature communicationsMay 3, 2022Zhang Q, Liu W, Wang H, Zhou H, Bulek K, Chen X, Zhang CJ, Zhao J, Zhang R, Liu C, et al.IL-10-PE (1:300, JES5-16E3, 505007) anti-mouse Ki67PE (1:300, 652404), anti-mouse CD3 PE/Cy7(1:300, 100220) and anti-mouse CD8 APC(1:300, 100712) were purchased from Biolegend. Anti-CD4-FITC (1:200, L3T4), Anti-Ly6C-PE

(20) Distinct Aβ pathology in the olfactory bulb and olfactory deficits in a mouse model of Aβ and α-syn co-pathologyBrain pathology (Zurich, Switzerland)May 1, 2022Friesen M, Ziegler-Waldkirch S, Egenolf M, d'Errico P, Helm C, Mezö C, Dokalis N, Erny D, Katzmarski N, Coelho R, et al.lymphoid cells by adding primary antibodies against anti‐CD3 (1:300, clone 17A2, Biolegend, cat. no. 100220), anti‐CD19 (1:300, clone 6D5, Biolegend, cat. no. 115520), anti‐CD45R (1:300, clone RA3‐6B2, BD Bioscience;

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12/29/2016Anti-Mouse CD3 PE-Cy7 Maria Carmela SperanzaCD3-PE-Cy7 was used to gate CD3- of CD45+cells extracted from spleenocytes, cLN and TILs of tumor-bearing mice, with the final aim to exclude the T-cell population from the analysis of the innate ... Read Review
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5/16/2016Anti-Mouse CD3 PECy7 Maria Carmela SperanzaThis is a good anti-mouse CD3 antibody. The experiments were performed using mouse splenocytes stimulated in vitro for 3 days with 0.5ng of OKT3. Standard protocol for staining. 1) Wash cells with ... Read Review

PE/Cyanine7 anti-mouse CD3 from BioLegend

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PE/Cyanine7 anti-mouse CD3 from BioLegend

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