Fig 1: Effect of VA treatment on the levels of CoQ biosynthetic proteins and mRNA levels in the MEFs and tissues of Coq9R239X mice.(A-D) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 (A), COQ4 (B), COQ5 (C) and COQ7 (D), and the quantitation of the protein bands in MEFs from Coq9+/+ and Coq9R239X mice after supplementation with VA at 1 mM(E-H) Relative mRNA levels for the genes Coq2 (E), Coq4 (F), Coq5 (G) and Coq7 (H) in MEFs from Coq9+/+ and Coq9R239X mice after supplementation with VA at 1 mM(I-L) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 (I), COQ4 (J), COQ5 (K) and COQ7 (L), and the quantitation of the protein bands in the brain of Coq9+/+ mice, Coq9+/+ mice after 1% VA treatment, Coq9R239X mice and Coq9R239X mice after 1% VA treatment.(M-P) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 (M), COQ4 (N), COQ5 (O) and COQ7 (P), and the quantitation of the protein bands in the kidney of Coq9+/+ mice, Coq9+/+ mice after 1% VA treatment, Coq9R239X mice and Coq9R239X mice after 1% VA treatment.(Q-T) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 (Q), COQ4 (R), COQ5 (S) and COQ7 (T), and the quantitation of the protein bands in the liver of Coq9+/+ mice, Coq9+/+ mice after 1% VA treatment, Coq9R239X mice and Coq9R239X mice after 1% VA treatment.(U and V) Relative mRNA levels of the genes Coq4 (U) and Coq5 (V) in brain from Coq9+/+ and Coq9R239X mice after the supplementation with VA at 1 mM(W and X) Relative mRNA levels of the genes Coq4 (W) and Coq5 (X) in the kidneys from Coq9+/+ and Coq9R239X mice after the supplementation with VA at 1 mM(Y and Z) Relative mRNA levels of the genes Coq4 (Y) and Coq5 (Z) in the liver from Coq9+/+ and Coq9R239X mice after the supplementation with VA at 1 mM.Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, differences versus Coq9+/+; +P < 0.05, ++P < 0.01, +++P < 0.001, versus Coq9R239X; (one-way ANOVA with a Tukey's post hoc test; n = 3 for each group for cell samples, n = 5 for each group for tissue samples).