Anti-CRISPR-Cas9 antibody ab204448 from Abcam

Product Specs
ItemAnti-CRISPR-Cas9 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab204448
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Conjugate/TagUnconjugated
IsotypeIgG
HostRabbit
Antibody TypePrimary
ClonalityPolyclonal
Quantity50 UL
NCBI Gene AliasesSPy1046
Gene Namecas9
ReactivityStreptococcus pyogenes
TargetCRISPR-Cas9

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Development of a versatile nuclease prime editor with upgraded precisionNature CommunicationsJanuary 19, 2023Xiangyang Li, Guiquan Zhang, Shisheng Huang, Yao Liu, Jin Tang, Mingtian Zhong, Xin Wang, Wenjun Sun, Yuan Yao, Quanjiang Ji, et al.were harvested and lysed in RIPA buffer. The blots were subjected to incubations with anti-Cas9 (Abcam ab204448, 1:2000), anti-β-actin (Absin abs132001, 1:10000), or anti-Flag (Abcam ab205606, EPR20018-251, 1:5000)

(2) Drug-gene Interaction Screens Coupled to Tumor Data Analyses Identify the Most Clinically Relevant Cancer Vulnerabilities Driving Sensitivity to PARP InhibitionCancer Res CommunOctober 1, 2022Jamal K, Galbiati A, Armenia J, Illuzzi G, Hall J, Bentouati S, Barrell D, Ahdesmäki M, Functional Genomics Centre Group, O'Connor MJ, et al.RRID:AB_1640207, 1:1,000), BRCA1 (Millipore, catalog no. OP92, RRID:AB_2750876, 1:1,000), Cas9 (Abcam, catalog no. ab204448, RRID:AB_2893352, 1:1,000), GAPDH (Cell Signaling Technology, catalog no. 2118, RRID:AB_561053, 1:1,000),

(3) Somatic Genetics Analysis of Sleep in Adult MiceThe Journal of NeuroscienceJuly 13, 2022Guodong Wang et al.anti-β-actin (Beyotime, AF003), anti-tubulin J (CST, 5568), anti-GFP (Beyotime, AG279), anti-Cas9 (Abcam, ab204448), anti-HA (Sigma, H6533), anti-GAPDH (EarthOx, E021010-01), HRP-labeled goat anti-rabbit IgG(H + L)

(4) Inhibition of base editors with anti-deaminases derived from virusesNature communicationsFebruary 1, 2022Liu Z, Chen S, Lai L, Li Z.Switzerland). The whole-cell lysate was used for immunoblotting. An antibody against Cas9 (1:1500; ab204448, Abcam) was used as a primary antibody, while an anti-tubulin antibody (1:2000; 10094‐1‐AP, Wuhan Sanying)

(5) Harnessing A3G for efficient and selective C-to-T conversion at C-rich sequencesBMC BiologyDecember 1, 2021Wenxia Yu, Jianan Li, Shisheng Huang, Xiangyang Li, Ping Li, Guanglei Li, Aibin Liang, Tian Chi, Xingxu Huang0.1% Tween 20) for 1 h, the membranes were incubated overnight with anti-CRISPR-Cas9 antibody (Abcam # ab204448) or anti-GAPDH antibody (Absin #abs132004) at 4 °C. After extensive washing, the membranes were incubated

(6) A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell linesScientific ReportsDecember 1, 2021Kathryn Rozen-Gagnon, Soon Yi, Eliana Jacobson, Sasha Novack, Charles M. Riceanti-Drosophila AGO1 ab5070 (1 µg/mL; Abcam), anti-Ae. aegypti AGO1 (1 µg/mL; generated in-house), anti-Cas9 ab204448 (1 µg/mL; Abcam), and anti-FLAG (0.5 µg/mL; monoclonal M2, Sigma-Aldrich). Following development membranes

(7) Hyperoxia causes senescence and increases glycolysis in cultured lung epithelial cellsPhysiological reportsMay 1, 2021Scaffa AM, Peterson AL, Carr JF, Garcia D, Yao H, Dennery PA.control for all western blots. Antibodies used were: Abcam: Anti‐H2AX phosphoS139 (ab26350), CRISPR‐Cas9 (ab204448), GLUT1 (ab115730), hexokinase II (HKII, ab131196), p16 (ab54210), p21 (ab107099), p53 (ab31333), phosphor‐p53

(8) A Targeted and Tuneable DNA Damage Tool Using CRISPR/Cas9BiomoleculesFebruary 15, 2021Ioannis Emmanouilidis, Natalia Fili, Alexander W. Cook, Yukti Hari-Gupta, Ália dos Santos, Lin Wang, Marisa L. Martin-Fernandez, Peter J. I. Ellis, Christopher P. ToselandTBS. The following antibodies were used at the indicated dilutions: Rabbit anti-Cas9 (1:200, Abcam ab204448), Mouse anti-phospho-H2A.X (1:500 Sigma, St. Louis, MO, USA 05-636), Donkey anti-rabbit Alexa Fluor

(9) Nanos3, a cancer-germline gene, promotes cell proliferation, migration, chemoresistance, and invasion of human glioblastomaCancer Cell InternationalDecember 1, 2020Fengyu Zhang, Ruilai Liu, Cheng Liu, Haishi Zhang, Yuan Lustreptomycin and 1% nonessential amino acids (Thermo, Carlsbad, CA, USA). Antibodies against Cas9 (Abcam, ab204448), Nanos3 (Abcam, ab70001), cyclin D1 (Abcam, ab40754), Gapdh (Abcam, ab37168) and α-tubulin (Abcam,

(10) Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusionsBMC biologyAugust 31, 2020Liu Z, Chen S, Shan H, Jia Y, Chen M, Song Y, Lai L, Li Z.with a protease inhibitor cocktail (Roche, Basel, Switzerland). The antibody against Cas9 (1:1500; ab204448, Abcam) was used as a primary antibody, while tubulin antibody (1:2000; 10094-1-AP, Wuhan Sanying) was

(11) A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexesScientific ReportsDecember 1, 2019A. St. Martin, D. J. Salamango, A. A. Serebrenik, N. M. Shaban, W. L. Brown, R. S. HarrisLicor Odyssey instrument. Primary antibodies used in these experiments were rabbit anti-Cas9 (Abcam ab204448) and mouse anti-HSP90 (BD Transduction Laboratories 610418). Secondary antibodies used were goat anti-rabbit

(12) SpCas9-expression by tumor cells can cause T cell-dependent tumor rejection in immunocompetent miceOncoimmunologyJanuary 1, 2019Ajina R, Zamalin D, Zuo A, Moussa M, Catalfamo M, Jablonski SA, Weiner LM.the following antibodies: CRISPR-Cas9 (EpiGentek #A-90000) (Figures 5b and 7a), CRISPR-Cas9 (Abcam #ab204448) (Figures 1a and 3b), Pan-keratin (Cell Signaling #4545), Tubulin (Abcam # ab6160) and GAPDH (D16H11)

(13) Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editingNature CommunicationsDecember 1, 2018Duverney Chaverra-Rodriguez, Vanessa M. Macias, Grant L. Hughes, Sujit Pujhari, Yasutsugu Suzuki, David R. Peterson, Donghun Kim, Sage McKeand, Jason L. Rasgon20. Following blocking, Cas9 protein was detected using rabbit anti-Cas9 polyclonal antibody (Abcam ab204448) diluted 1:500 in PBS with 0.1% Tween 20 and incubated for 1 h at RT or overnight at 4 °C. After incubation,

(14) A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cellsNucleic Acids ResearchAugust 21, 2018Amber St. Martin, Daniel Salamango, Artur Serebrenik, Nadine Shaban, William L Brown, Francesco Donati, Uday Munagala, Silvestro G Conticello, Reuben S HarrisLICOR Odyssey instrument. Primary antibodies used in these experiments were rabbit anti-Cas9 (Abcam ab204448) and mouse anti-HSP90 (BD Transduction Laboratories 610418). Secondary antibodies used were goat anti-rabbit

(15) Contracting CAG/CTG repeats using the CRISPR-Cas9 nickaseNature CommunicationsNovember 9, 2016Cinzia Cinesi, Lorène Aeschbach, Bin Yang, Vincent Dion

(16) Repurposing the CRISPR-Cas9 system for targeted DNA methylationNucleic acids researchJuly 8, 2016Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, Klasić M, Zoldoš V.KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.4), then incubated overnight with anti-Cas9 antibody 1:2000 (ab204448; Abcam) in 5% milk-PBS containing 0.2% Tween 20, followed by incubation with anti-rabbit HRP-conjugated

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Anti-CRISPR-Cas9 antibody from Abcam

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Anti-CRISPR-Cas9 antibody from Abcam

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