Fig 1: (A) Western blot analysis of proteins in mouse myometrial smooth muscle cells with and without TRPC3-siRNA transfection. GAPDH was used as the loading control. Quantified protein expression levels of (B) TRPC3, (C) PLC?, (D) PKC, (E) CPI-17, (F) P-CPI-17, (G) Cav1.2, (H) Cav3.1 and (I) Cav3.2. Data are presented as the mean ± standard error. *P<0.05 vs. CON group. CON, untransfected non-infected with LPS; LPS, infected with LPS; siTRPC3 CON, transfected with siTRPC3 and non-infected with LPS; siTRPC3 LPS, transfected with siTRPC3 and infected with LPS. TRPC3, canonical transient receptor potential 3; siRNA, small interfering RNA; PLC, phospholipase C; PKC, protein kinase C; CPI-17, C-kinase-activated protein phosphatase-1 inhibitor; LPS, lipopolysaccharides; CON, control.
Fig 2: Expression of Cav3.1 protein with and without TRPC3-siRNA transfection in murine uterine smooth muscle cells. CON, untransfected non-infected with LPS; LPS, infected with LPS; siTRPC3 CON, transfected with siTRPC3 and non-infected with LPS; siTRPC3 LPS, transfected with siTRPC3 and infected with LPS. TRPC3, canonical transient receptor potential 3; siRNA, small interfering RNA; LPS, lipopolysaccharides; CON, control.
Supplier Page from Abcam for Anti-CACNA1G antibody