Fig 1: The reduction of protein O-GlcNAcylated proteins is cell-type specific in the hippocampus of Ts2Cje mice. (A1–8) O-GlcNAc co-localization with the neuronal marker NeuN-1 in the CA3 hippocampal area from Ts2 and respective euploid mice. O-GlcNAc signal seems to broadly overlap with neuronal marker NeuN-1 in both CA3 area from euploid animals (A1–4) and Ts2 mice (A5–8). Co-localization graphs are reported for both Eu (A4) and Ts2 (A8) mice; O-GlcNAc (green); NeuN-1 (red); co-localization (yellow). (B1–8) O-GlcNAc co-localization with glial marker IBA-1 in the CA3 hippocampal area from Ts2 and respective euploid mice. Partial co-localization was observed between O-GlcNAcylated proteins and fluorescent signal from the glial marker IBA-1 in Ts2 CA3 subregion (B1–4) and a comparable result was obtained in the same region of euploid animals (B5–8). Co-localization graphs are reported for both Eu (B4) and Ts2 (B8) mice; O-GlcNAc (green); IBA-1 (red); co-localization (yellow). (C1–8) O-GlcNAc co-localization with the astrocytic marker GFAP in the CA3 hippocampal area from Ts2 and respective euploid mice. Consistent co-localization of O-GlcNAc signal and GFAP was detected in the CA3 area of euploid animals (C1–4), while apparently no signal has been identified in the same area of Ts2 hippocampus (C5–8). Co-localization graphs are reported for both Eu (C4) and Ts2 (C8) mice; O-GlcNAc (green); GFAP (red); co-localization (yellow). Number of animals for each condition was as follows: n = 3/group for immunofluorescence staining