Purified anti-HA.11 Epitope Tag 901501 from BioLegend

Product Specs
ItemPurified anti-HA.11 Epitope Tag
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number901501
Quantity200 µL
ApplicationsWestern Blot (WB), Flow Cytometry (FC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Purification
Concentration1.00 mg/mL
Conjugate/TagUnconjugated
FormatPhosphate-buffered solution + 50% Glycerol + 0.03% Thimerosal
IsotypeIgG1, Kappa
Clone16B12
HostMouse
ImmunogenMonoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL
ClonalityMonoclonal
Brand/TrademarkMMS-101P-200
TargetHA.11

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) The NSL complex is required for piRNA production from telomeric clustersLife Science AllianceSeptember 1, 2023Shantanu S Iyer, Yidan Sun, Janine Seyfferth, Vinitha Manjunath, Maria Samata, Anastasios Alexiadis, Tanvi Kulkarni, Noel Gutierrez, Plamen Georgiev, Maria Shvedunova, et al.anti-HA,Mouse,1:400 (IF),901501 (BioLegend) anti-FLAGHRP,mouse,"1:10,000 (WB)","clone M2, A8592 (Sigma-Aldrich)" anti-Piwi,mouse,"1:2000

(2) RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursorsbioRxivJuly 28, 2023Dan Dou, Jayne Aiken, Erika L.F. Holzbaur1:200 Anti-βIII-tubulin,"Abcam (Cat# ab7751, RRID:AB_306045)",ICC @ 1:500 "Anti-HA, Mouse Monoclonal","BioLegend (Cat# 901501, RRID:AB_2565006)",WB @ 1:2000 "Anti-GFP, Chicken Polyclonal","AvesLabs (Cat# GFP-1020, RRID:AB_10000240)",WB

(3) Forkhead transcription factor FKH-8 cooperates with RFX in the direct regulation of sensory cilia in Caenorhabditis eleganseLifeJuly 14, 2023Rebeca Brocal-Ruiz, Ainara Esteve-Serrano, Carlos Mora-Martínez, Maria Luisa Franco-Rivadeneira, Peter Swoboda, Juan J Tena, Marçal Vilar, Nuria Flamesthe heavy chain from the immunoprecipitation would mask the FKH-8 signal [anti-HA antibody (Biolegend 901501), anti-FLAG antibody (Sigma, F1804)]. Cytoplasmic and nuclear fractions were controlled using antibodies

(4) The ribosome quality control factor Asc1 determines the fate of HSP70 mRNA on and off the ribosomeNucleic Acids ResearchJuly 7, 2023Lokha R Alagar Boopathy, Emma Beadle, Alan RuoChen Xiao, Aitana Garcia-Bueno Rico, Celia Alecki, Irene Garcia de-Andres, Kyla Edelmeier, Luca Lazzari, Mehdi Amiri, Maria Vera(eIF2α, phospho eIF2α (Ser51) (Cat# 9722, 9721S, Cell Signaling Technology, Danvers, MA, USA)), HA (Cat# 901501, BioLegend), tubulin (Developmental Studies Hybridoma Bank), and β-actin (Cat# A2228, EMD Millipore

(5) Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformationNature communicationsJuly 3, 2023Calcagni' A, Staiano L, Zampelli N, Minopoli N, Herz NJ, Di Tullio G, Huynh T, Monfregola J, Esposito A, Cirillo C, et al.domains. Reagents used in this study were obtained from the following sources: HA (Biolegend, Cat# 902301; Cat# 901501, 1:400 dilution for IF, 1:1000 for WB), LAMP1 (Santa Cruz, Cat# sc-20011, 1:200 dilution for IF, 1:1000

(6) Reprogramming of iron metabolism confers ferroptosis resistance in ECM-detached cellsiScienceJune 16, 2023He J, Abikoye AM, McLaughlin BP, Middleton RS, Sheldon R, Jones RG, Schafer ZT.(Proteintech; 20886-1-AP; 1:1000), xCT (CST; 12691), p-Yap S127 (CST; 13008T; 1:1000), HA-Tag (Biolegend; 901501; 1:2000), FLAG Tag (CST; 2368S; 1:1000), TfR1 (CST; 13113S; 1:1000). Poly-HEMA coating Poly-HEMA (Sigma;

(7) The debranching enzyme Dbr1 regulates lariat turnover and intron splicingResearch squareJune 13, 2023Buerer L, Clark NE, Welch A, Duan C, Taggart AJ, Townley BA, Wang J, Soemedi R, Rong S, Lin CL, et al.(mouse),Santa Cruz Biotechnology,Cat#SC-47724 AQR (rabbit),Bethyl Labs,Cat#A302-546A HA (mouse),BioLegend,Cat#901501 Secondary goat anti-mouse Alexa Fluor 647,Thermo Fisher Scientific,Cat#A-21246 "Chemicals, peptides,

(8) Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranesNature communicationsJune 9, 2023Hildebrandt RP, Moss KR, Janusz-Kaminska A, Knudson LA, Denes LT, Saxena T, Boggupalli DP, Li Z, Lin K, Bassell GJ, et al.ab125903), rabbit anti-HA (1:800, Cell Signaling Technologies, C29F4), and mouse anti-HA (1:1000, BioLegend, 901501). Cells were then washed 3X with PBS and incubated with secondary antibody in AB buffer (anti-mouse

(9) RagD auto-activating mutations impair MiT/TFE activity in kidney tubulopathy and cardiomyopathy syndromeNature communicationsMay 15, 2023Sambri I, Ferniani M, Campostrini G, Testa M, Meraviglia V, de Araujo MEG, Dokládal L, Vilardo C, Monfregola J, Zampelli N, et al.4240S 1:200), Phospho-TFEB (Ser211) (Cell Signaling cat. 37681 1:200), HA.11 clone 16B12 (BioLegend 901501 1:500) Parkin (Cell Signaling cat 2132 1:200), Tomm20 (BD cat n 612278 1:800), Galectin-3 (M3/38 sc-23938

(10) Generation of a Yeast Cell Model Potentially Useful to Identify the Mammalian Mitochondrial N-Acetylglutamate TransporterBiomoleculesMay 10, 2023Ruggiero Gorgoglione, Roberta Seccia, Amer Ahmed, Angelo Vozza, Loredana Capobianco, Alessia Lodi, Federica Marra, Eleonora Paradies, Luigi Palmieri, Vincenzo Coppola, et al.Cell Signaling Technology, Leiden, The Netherlands) or anti-HA.11 Epitope Tag Antibody clone 16B12 (1:5000 catalogue no. 901501; BioLegend, London, UK) primary antibody overnight at 4 °C. A rabbit antiserum against yeast Aac2p,

(11) The proprotein convertase SKI-1/S1P is a critical host factor for Nairobi sheep disease virus infectivityVirus ResearchMay 1, 2023Caroline Bost, Julia Hartlaub, Vinícius Pinho dos Reis, Thomas Strecker, Nabil G. Seidah, Martin H. Groschup, Sandra Diederich, Kerstin Fischer(Sigma-Aldrich; 1:500). Precipitated proteins were detected in WB with monoclonal HA.11 antibodies (#901501, Biolegend; 1:1000). Secondary anti-mouse and anti-rabbit antibodies conjugated either with Horseradish

(12) Spatial and temporal dynamics of ATP synthase from mitochondria toward the cell surfaceCommunications BiologyApril 18, 2023Yi-Wen Chang, T. Tony Yang, Min-Chun Chen, Y-geh Liaw, Chieh-Fan Yin, Xiu-Qi Lin-Yan, Ting-Yu Huang, Jen-Tzu Hou, Yi-Hsuan Hung, Chia-Lang Hsu, et al.Cat# GTX628802, 1:500), ATP synthase β subunit (GeneTex Cat# GTX84845, 1:1000), HA-tag (BioLegend Cat# 901501, 1:1000), E-cadherin (Merck Millipore Cat# MAB3199Z, 1:200), and Actin (Merck Millipore Cat# MAB1501,

(13) LMAN1 is a receptor for house dust mite allergensCell reportsMarch 28, 2023Miller MH, Swaby LG, Vailoces VS, LaFratta M, Zhang Y, Zhu X, Hitchcock DJ, Jewett TJ, Zhang B, Tigno-Aranjuez JT.M2 (mouse)",Sigma-Aldrich,Cat# F1804; RRID:AB_262044 "α-HA-11, clone 16B12 (mouse)",Biolegend,Cat# 901501; RRID:AB_2565006 "α-LMAN1, clone OTI1A8 (mouse) – used with secondary reagents for quantitation of

(14) An essential endoplasmic reticulum-resident N-acetyltransferase ortholog in Plasmodium falciparumJournal of Cell ScienceMarch 15, 2023Alexander J. Polino, Muhammad M. Hasan, Katherine Floyd, Yolotzin Avila-Cruz, Yujuan Yang, Daniel E. GoldbergPolino et al. (2020) with primary antibodies mouse anti-HA diluted 1:1000 (clone 16B12; Biolegend, 901501), rabbit anti-HA diluted 1:1000 (Sigma-Aldrich, H6908), rabbit anti-PfAldolase diluted 1:2000 (Abcam,

(15) The clathrin adaptor complex-1 and Rab12 regulate post-Golgi trafficking of wild-type epidermal growth factor receptor (EGFR)Journal of Biological ChemistryMarch 1, 2023Jinhui Wang, Pik Ki Lau, Chun Wa Li, Yusong Guorabbit anti-HA (Cell Signaling #3724, RRID: AB_1549585), mouse anti-HA (Biolegend, catalogue number 901501), sheep anti-TGN46 (AbD Serotec, number AHP500G, RRID: AB_323104), mouse anti-FLAG (Sigma-Aldrich, number

(16) The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genesThe Journal of biological chemistryMarch 1, 2023Song L, Wang D, Abbas G, Li M, Cui M, Wang J, Lin Z, Zhang XE.Mouse (Ms) anti-FLAG (Sigma-Aldrich, F1804), Ms anti-HA (Cell Signaling, 2367), Ms anti-HA (Biolegend, 901501), Ms HRP-conjugated anti-Alpha Tubulin (Proteintech, HRP-66031), Ms HRP-conjugated anti-GAPDH (Yesen,

(17) Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA moduleScience advancesFebruary 22, 2023Wang S, Chen X, Crisman L, Dou X, Winborn CS, Wan C, Puscher H, Yin Q, Kennedy MJ, Shen J.bath, incubated with KRH buffer containing 5% FBE, and labeled with anti-HA antibodies (BioLegend, #901501, RRID: AB_2565006) and allophycocyanin (APC)–conjugated secondary antibodies (eBioscience, #17-4015-82).

(18) The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartmentsNature CommunicationsFebruary 22, 2023Wenyan Wan, Hui Dong, De-Hua Lai, Jiong Yang, Kai He, Xiaoyan Tang, Qun Liu, Geoff Hide, Xing-Quan Zhu, L. David Sibley, et al.antibodies, 71-5500, Thermo Fisher Scientific, dilution: 1:500; mouse anti-HA, anti-HA.11, clone 16B12, 901501, Biolegend, dilution: 1:500) and C-myc (mouse anti-c-myc, mAB 9E10, MA1-980, Thermo Fisher Scientific,

(19) SREBP modulates the NADP+/NADPH cycle to control night sleep in DrosophilaNature CommunicationsFebruary 20, 2023Vittoria Mariano, Alexandros K. Kanellopoulos, Giuseppe Aiello, Adrian C. Lo, Eric Legius, Tilmann Achsel, Claudia Bagni(DHSB, clone nc82, RRID: AB_2314866, 1:1000), and anti-HA 11 epitope tag (BioLegend, clone 16B12, cat. 901501, RRID: AB_2801249, 1:1000). The following secondary antibodies conjugated with Alexa Fluor (Thermo Fisher Scientific

(20) Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2Nature communicationsFebruary 16, 2023Nalbach K, Schifferer M, Bhattacharya D, Ho-Xuan H, Tseng W, Williams LA, Stolz A, Lichtenthaler SF, Elazar Z, Behrends C.GM130 (Abcam, #ab52649), GOLIM4 (Abcam, #ab28049), HA.11 Clone 16B2 (Covance/Biolegend, #MMS-101P/#901501), HS6ST2 (Abcam, # ab122220), LAMP1 (IF 1:100, Abcam/DSHB, #ab24170/H4A3), LAMP2 (IF 1:100, Abcam, #ab25631),

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(1)

6/7/2019Great HA.11 Epitope Tag Antibody from BioLegend Xu-Qiao ChenHigh titer and clean. Overall, it is a reliable antibody for western blot.
(1)

12/22/2015HA Antibody for Western Blotting Ganesh PusapatiThis antibody was used to test the expression of a HA tagged protein overexpressed in HEK293T cells. The end goal of this experiment was to use this antibody to test the interaction of a HA tagged ... Read Review

Purified anti-HA.11 Epitope Tag from BioLegend

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Purified anti-HA.11 Epitope Tag from BioLegend

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