Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190 from Abcam

Product Specs
ItemMouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab170190
Quantity100 UG
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry paraffin embedded sections
Concentration1 mg/ml
Conjugate/TagUnconjugated
IsotypeIgG1
HostMouse, Nude mouse
ClonalityMonoclonal
Antibody TypePrimary
Clone15-6E10A7
NCBI Gene AliasesHSPC065
Gene Nameccdc113
TargetCoiled-coil domain-containing protein 113

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Website: http://www.abcam.com

(1) Ionizing radiation normalizes the features of active breast cancer stromal fibroblasts and suppresses their paracrine pro-carcinogenic effectsTranslational OncologyNovember 1, 2023Abdelilah Aboussekhra, Noura N. Alraouji, Falah H. Al-Mohanna, Huda Al-Khalafdescribed [18]. Antibodies directed against, α-SMA (ab5694), TGF-β1 (ab-64,715), SDF-1 (ab-10,395), Twist1 (ab170190), IL-6 (ab-9324), Lamin B1 (ab8982) and Vimentin (ab-8978) were purchased from Abcam (Cambridge, MA).

(2) Bio-Enhanced Neoligaments Graft Bearing FE002 Primary Progenitor Tenocytes: Allogeneic Tissue Engineering & Surgical Proofs-of-Concept for Hand Ligament Regenerative MedicinePharmaceuticsJuly 3, 2023Annick Jeannerat, Joachim Meuli, Cédric Peneveyre, Sandra Jaccoud, Michèle Chemali, Axelle Thomas, Zhifeng Liao, Philippe Abdel-Sayed, Corinne Scaletta, Nathalie Hirt-Burri, et al.control antibody: Abcam Ref. N°ab172730 (1:100 dilution)Mouse isotype control antibody: Abcam Ref. N°ab170190 (1:100 dilution)Secondary anti-rabbit Alexa 488 antibody: Abcam Ref. N°ab150081 (1:250 dilution)Secondary

(3) Dynamic interactome of the MHC I peptide loading complex in human dendritic cellsProceedings of the National Academy of SciencesJune 20, 2023Martina Barends, Nicole Koller, Christian Schölz, Verónica Durán, Berislav Bošnjak, Jennifer Becker, Marius Döring, Hanna Blees, Reinhold Förster, Ulrich Kalinke, et al.either with 15 µg purified TAP1 antibody (mAb 148.3) or with 15 µg isotype control antibody (Abcam, ab170190). Samples were incubated for 3 h at 4 °C on an overhead rotator. Afterward, beads were washed three

(4) METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cellsCell Death DiscoveryDecember 1, 2022Renbing Jiang, Zhibing Dai, Junshen Wu, Suzhi Ji, Yachao Sun, Wenpeng Yangextracted using TRIzol reagent. Then, RNA was incubated with anti-m6A (ab208577, Abcam) or anti-IgG (ab170190, Abcam) and protein A/G magnetic beads in IP buffer overnight. After ethanol precipitation, the immunoprecipitated

(5) SIRT3-Mediated CypD-K166 Deacetylation Alleviates Neuropathic Pain by Improving Mitochondrial Dysfunction and Inhibiting Oxidative StressOxidative Medicine and Cellular LongevitySeptember 1, 2022Binbin Yan, Qiang Liu, Xiaobao Ding, Yuwen Lin, Xiaowei Jiao, Yuqing Wu, Huihui Miao, Chenghua Zhouprimary antibodies that against SIRT3 (1 : 1000, D22A3, Cell Signaling Technology, USA), CypD (1 : 1000, ab170190, Abcam, UK), Ac-CypD-K166 (1 : 1000, WG-00545P, ABclonal, China), MnSOD (1 : 1000, ET1701-54, HUABIO,

(6) Heterogeneous nuclear ribonucleoprotein U-actin complex derived from extracellular vesicles facilitates proliferation and migration of human coronary artery endothelial cells by promoting RNA polymerase II transcriptionBioengineeredMay 10, 2022Han Wang, Hengdao Liu, Xi Zhao, Xiaowei Chenimmunoglobulin G (IgG) Alexa Fluor® 488 (1:2000, ab150113, Abcam) for 30 min. The mouse IgG (1 µg/mL, ab170190, Abcam) served as the isotype control. After two washes, cells were resuspended in phosphate-buffered

(7) Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblastsThe Journal of experimental medicineFebruary 7, 2022Kerdidani D, Aerakis E, Verrou KM, Angelidis I, Douka K, Maniou MA, Stamoulis P, Goudevenou K, Prados A, Tzaferis C, et al.ab24733) or isotypic controls (mouse IgG2a [Biolegend; cat. no. 361702] and mouse IgG1 [Abcam; cat. no. ab170190], respectively). Cells were co-cultured in complete RPMI (Gibco) medium. Classical Ficoll-Pague (StemCell;

(8) Resolving the immune landscape of human prostate at a single-cell level in health and cancerCell reportsDecember 21, 2021Tuong ZK, Loudon KW, Berry B, Richoz N, Jones J, Tan X, Nguyen Q, George A, Hori S, Field S, et al.rabbit),Bioss,Cat#bs-2431R; RRID: AB_10855666 Mouse IgG1 kappa monoclonal isotype control,Abcam,Cat#ab170190; clone 15-6E10A7; RRID: AB_2736870 "Goat anti-mouse IgG secondary (FITC, polyclonal)",Invitrogen,Cat#31569;

(9) Human Erbb2-induced Erk activity robustly stimulates cycling and functional remodeling of rat and human cardiomyocyteseLifeOctober 19, 2021Nicholas Strash, Sophia DeLuca, Geovanni L Janer Carattini, Soon Chul Heo, Ryne Gorsuch, Nenad Bursac,Santa Cruz Biotechnology,sc-47724,WB (1:1000) Antibody,"Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control",Abcam,ab170190,FC (1:2000) Antibody,Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Rabbit polyclonal),Cell Signaling Technology

(10) Intestinal Host Response to SARS-CoV-2 Infection and COVID-19 Outcomes in Patients With Gastrointestinal SymptomsGastroenterologyJune 1, 2021Livanos AE, Jha D, Cossarini F, Gonzalez-Reiche AS, Tokuyama M, Aydillo T, Parigi TL, Ladinsky MS, Ramos I, Dunleavy K, et al.control),Polyclonal,Abcam,ab37415,Rabbit,Unconjugated,variable Yeast GAL4 (isotype control),15-6E10A7,Abcam,ab170190,Mouse,Unconjugated,variable Mouse IgG Heavy and light chains,Polyclonal,Abcam,ab150116,Goat,Alexa Fluor

(11) A Novel Rabbit Dry Eye Model Induced by a Controlled Drying SystemTranslational Vision Science & TechnologyMay 18, 2021Xiao-Min Chen, Jian-Biao Kuang, Hui-Yin Yu, Zhen-Ning Wu, Shu-Yi Wang, Shi-You Zhouthe specificity of anti-MUC5AC antibody, the antibody was substituted by isotype control antibody (ab170190; Abcam) to finish the control experiment. The specimens were examined at 200 × magnification by an epifluorescence

(12) Evolution of antibody immunity to SARS-CoV-2NatureMarch 1, 2021Gaebler C, Wang Z, Lorenzi JCC, Muecksch F, Finkin S, Tokuyama M, Cho A, Jankovic M, Schaefer-Babajew D, Oliveira TY, et al.M. et al., J Virol, PMID: 15681410) Rabbit isotype control Abcam ab37415 Mouse anti-Yeast GAL4 Abcam Ab170190 Goat anti-Mouse IgG Alexa Fluor 594 Abcam ab150116 Goat anti-Rabbit IgG Alexa Fluor 488 Abcam ab150077 Validation Rabbit

(13) Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs) and Dental Pulp Stem Cells (DPSCs) Display a Similar Profile with PericytesStem cells internationalJanuary 1, 2021Zhu SY, Yuan CY, Lin YF, Liu H, Yang YQ, Wong HM, Zhang CF, Wang PL, Gu M.Abcam) and goat anti-rabbit IgG H&L (Alexa Fluor® 488). In this study, mouse IgG1, kappa monoclonal (ab170190; Abcam), and rabbit IgG monoclonal [EPR25A] (ab172730; Abcam) were used as isotype controls. Flow cytometry

(14) Methylprednisolone alleviates multiple sclerosis by expanding myeloid-derived suppressor cells via glucocorticoid receptor β and S100A8/9 up-regulationJournal of cellular and molecular medicineDecember 1, 2020Wang Z, Zheng G, Li G, Wang M, Ma Z, Li H, Wang XY, Yi H.IgG, polyclonal (ab171870; Abcam), Rabbit IgG, monoclonal (ab199376; Abcam), and Mouse monoclonal IgG1(ab170190; Abcam) from Abcam. For intracellular staining, cells were first stained for surface antigens, fixed,

(15) Modelling the catabolic environment of the moderately degenerated disc with a caprine ex vivo loaded disc culture systemEuropean Cells and MaterialsJuly 17, 2020C Me Rustenburg, J W Snuggs, K S Emanuel, A Thorpe, C Sammon, C L Le Maitre, T H Smitloaded disc culture system primary antibodies at equal protein concentrations (mouse IgG control: ab170190; rabbit IgG control: ab37415; Abcam). Following washes in TBS, slides were incubated with biotinylated

(16) Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidanceNature communicationsFebruary 28, 2020Martens R, Permanyer M, Werth K, Yu K, Braun A, Halle O, Halle S, Patzer GE, Bošnjak B, Kiefer F, et al.mouse monoclonal Anti-Talin1 (8D4) (# ab157808; 1/200) and Mouse IgG1 isotype control (15-6E10A7) (# ab170190; 1/200) (both from Abcam); PerCP anti-mouse CD4 (RM4-5) (# 100537; 1/200), APC anti-mouse CD62L (MEL-14)

(17) Novel Targets of Protoporphyrin-IX Determined By Gene Expression AnalysisInternational Journal of Clinical Oncology and Cancer ResearchJanuary 1, 2020Yiyang Dai, Wolfgang Kemmnermouse monoclonal (ab57043 from Abcam, Berlin, Germany). Isotype control was a monoclonal Mouse IgG1 (ab170190, Abcam). Cells were incubated overnight in the dark at 4°C. Cells were washed by centrifugation at 400

(18) Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell culturesJournal of Cellular PhysiologyJune 1, 2019Han Deng, Sumona Mondal, Shantanu Sur, Craig D. Woodworthirrelevant primary antibodies (Abcam Cat# ab172730, RRID:AB_2687931, rabbit monoclonal isotype; Abcam Cat# ab170190, RRID:AB_2736870, mouse monoclonal isotype) were used as negative controls. The dilution factor for

(19) Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve RegenerationStem Cells InternationalMay 2, 2019Feng Rao et al.CD90 (FITC) (ab124527; Abcam), and CD146 (FITC) (ab78451; Abcam) were used. Mouse monoclonal IgG1 (ab170190; Abcam) isotype was used as control. 2.3. Multipotent Diﬀerentiation of GMSCs 2.3.1. Osteogenic Diﬀerentiation.

(20) PEGylated TiO₂nanoparticles mediated inhibition of cell migration via integrin beta 1Science and Technology of Advanced MaterialsDecember 31, 2018Qingqing Sun, Koki Kanehira, Akiyoshi TaniguchiCambridge, USA); isotype control: rabbit IgG isotype control (Invitrogen, Waltham, MA, USA) and mouse IgG (ab170190, Abcam, Cambridge, MA, USA). Negative controls were performed using the respective secondary antibodies

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Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control from Abcam

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Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control from Abcam

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