Anti-beta Tubulin antibody [EPR16774] ab179513 from Abcam

Product Specs
ItemAnti-beta Tubulin antibody [EPR16774]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab179513
Quantity10 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
IsotypeIgG
CloneEPR16774
HostRabbit
Antibody TypePrimary
ClonalityMonoclonal
NCBI Gene AliasesCDCBM7, PMGYSA, bA506K6.1
Gene NameTubb2b
ReactivityAfrican green monkey, Chicken, Cow, Dog, Drosophila melanogaster, Human, Mouse, Rat, Recombinant fragment, Xenopus tropicalis, Zebrafish
Targetbeta Tubulin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Maf1 controls retinal neuron number by both RNA Pol III- and Pol II-dependent mechanismsiScienceDecember 15, 2023Yifei Li, Dongchang Xiao, Haiqiao Chen, X.F. Steven Zheng, Mengqing Xiangwere used: anti-MAF1(GeneTex, GTX106776), anti-RFP (Rockland, 600-401-379) and anti-β-tubulin (Abcam, ab179513). The dilution factors were 1:20 for Maf1 and RFP and 1:100 for β-tubulin. The Compass software (ProteinSimple,

(2) Cav3.2 T-Type calcium channels downregulation attenuates bone cancer pain induced by inhibiting IGF-1/HIF-1α signaling pathway in the rat spinal cordJournal of Bone OncologyOctober 1, 2023Qingying Liu, Zhongyuan Lu, Huan Ren, Lijun Fu, Yueliang Wang, Huilian Bu, Minyu Ma, Letian Ma, Chen Huang, Jian Wang, et al.Cell Signaling Technology), rabbit anti-HIF-1α (1: 2000, 20960-1-AP, Proteintech), rabbit anti-β-tubulin (1: 1000, ab179513, Abcam), or rabbit anti- Histone-H3 (1: 3000, 17168-1-AP, Proteintech). Subsequently, after washing

(3) Dynamic 3D morphology of chick embryos and allantois depicted nondestructively by 3.0T clinical magnetic resonance imagingPoultry ScienceSeptember 1, 2023Lei Chen, Zhongqiang Wang, Xubin Fu, Shuncong Wang, Yuanbo Feng, Walter Coudyzer, Shugeng Wu, Haijun Zhang, Zhihong Chai, Yue Li, et al.goat serum to block specimens for 30 min; 2) using the primary antibody for anti-β-tubulin (ABCAM, ab179513) in 1:50 to incubate specimens overnight; 3) using a secondary antibody, fluorescein isothiocyanate

(4) P65 mediated UBR4 in exosomes derived from menstrual blood stromal cells to reduce endometrial fibrosis by regulating YAP UbiquitinationJournal of NanobiotechnologyAugust 29, 2023Jiarui Qi, Xudong Zhang, Siwen Zhang, Shanshan Wu, Yimeng Lu, Shuyu Li, Pingping Li, Jichun Tanantibody. Next the PVDF membrane was incubated with UBR4 (1:500, ab86438, Abcam, USA), β-tubulin (1:3000, ab179513, Abcam, USA), CD81 (1:1000, ab79559, Abcam, USA), COL I (1:2000, ab34710, Abcam, USA), YAP (1:2000,

(5) Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate paclitaxel-induced spermatogenesis defects and maintain male fertilityBiological ResearchAugust 13, 2023YuSheng Zhang, YaNan Liu, Zi Teng, ZeLin Wang, Peng Zhu, ZhiXin Wang, FuJun Liu, XueXia Liuab97672, Bcl-2-associated X protein (BAX), ab32503; B-cell lymphoma 2 (BCL2), ab182858; β-Tubulin (TUBB), ab179513, Abcam, Cambridge, UK; MutL homolog 1 (MLH1), D221003; DNA meiotic recombinase 1 (DMC1), D224646; Meiotic

(6) Extracellular vesicle-circEHD2 promotes the progression of renal cell carcinoma by activating cancer-associated fibroblastsMolecular CancerJuly 22, 2023Tao He, Qiansheng Zhang, Peng Xu, Wen Tao, Fuyang Lin, Renfei Liu, Mingzhao Li, Xiaolu Duan, Chao Cai, Di Gu, et al.1:1000), TSG101 (ab125011, 1:1000), α-SMA (ab32575, 1:1000), hnRNPA2B1 (ab31645,1:500), β-Tubulin (ab179513, 1:1000), GAPDH (ab181602, 1:5000), and H3 (ab32356, 1:2000) were obtained from Abcam, and the secondary

(7) Euscaphis japonica Kanitz Fruit Exerts Antiobesity Effects by Inhibiting the Early Stage of Adipogenic DifferentiationNutrientsJuly 8, 2023Eunbi Lee, Juhye Park, Ju-Ock Namantibody PPARγ,SCBT,sc-7273,M,1:500 C/EBPα,CST,2295S,R,1:1000 IRS1,CST,2390S,R,1:1000 p-IRS1,Invitrogen,2103503,R,1:1000 Akt,CST,4691S,R,1:1000 p-Akt,CST,4060S,R,1:1000 β-actin,SCBT,sc-47778,M,1:500 β-tubulin,Abcam,ab179513,R,1:1000 Secondary antibody,Secondary antibody,Secondary antibody,Secondary antibody,Secondary antibody Mouse,CST,7076S,,1:2000 Rabbit,CST,7074S,,1:3000 Antibody

(8) Downregulation of HAS‑2 regulates the chondrocyte cytoskeleton and induces cartilage degeneration by activating the RhoA/ROCK signaling pathwayInternational journal of molecular medicineJuly 1, 2023Yang J, Wang L, Zhang Z, Sun Q, Zhang Y.DAPI for 5 min at room temperature. Tubulin was stained with anti-β tubulin antibody (1:400; cat. no. ab179513; Abcam) overnight at 4°C and goat anti-rabbit IgG H&L (Alexa Fluor 488) (1:200) for 1 h at room temperature

(9) Moesin is an effector of tau-induced actin overstabilization, cell cycle activation, and neurotoxicity in Alzheimer''s diseaseiScienceMarch 17, 2023Beckmann A, Ramirez P, Gamez M, Gonzalez E, De Mange J, Bieniek KF, Ray WJ, Frost B.,Thermo Scientific,"MN1020, RRID:AB_223647" shotgun,Developmental Studies Hybridoma Bank,DCAD2 β-tubulin,Abcam,ab179513 Alexa Fluor 488 (Mouse),Invitrogen,A21042 Alexa Fluor 488 (Rabbit),Invitrogen,A11034 Alexa Fluor

(10) Exosome-based bone-targeting drug delivery alleviates impaired osteoblastic bone formation and bone loss in inflammatory bowel diseasesCell reports. MedicineJanuary 17, 2023Guo J, Wang F, Hu Y, Luo Y, Wei Y, Xu K, Zhang H, Liu H, Bo L, Lv S, et al.p-IKKβ,Affinity,AF3013 Anti-mouse LaminB1,Abcam,Cat# ab133741; RRID:AB_2616597 Anti-mouse Tubulin,Abcam,Cat# ab179513 Anti-mouse GAPDH,Abcam,Cat# ab181602; RRID:AB_2630358 Anti-rabbit IgG (H&L) (DyLight™ 800 4X PEG Conjugate),Cell Signaling Technology

(11) Development of p-Tau Differentiated Cell Model of Alzheimer’s Disease to Screen Novel Acetylcholinesterase InhibitorsInternational Journal of Molecular SciencesNovember 26, 2022Giuseppe Uras, Xinuo Li, Alessia Manca, Antonella Pantaleo, Marco Bo, Jinyi Xu, Stephanie Allen, Zheying Zhusolution and incubated overnight at +4 °C with the mouse monoclonal anti-tubulin III primary antibody (#ab179513, Abcam plc., Camabridge, UK) at a final concentration of 1:1000. The primary antibody was then removed,

(12) Circadian gene Rev-erbα influenced by sleep conduces to pregnancy by promoting endometrial decidualization via IL-6-PR-C/EBPβ axisJournal of Biomedical ScienceNovember 24, 2022Liyuan Cui, Feng Xu, Chunfang Xu, Yan Ding, Songcun Wang, Meirong Du(ab180948, Abcam), anti-Rev-erbα (sc-393215, Santa Cruze), anti-β-Actin (ab179467, Abcam), anti-β-Tubulin (ab179513, Abcam), anti-PR (human, 8757, Cell Signaling Technology), anti-C/EBPβ (ab32358, Abcam); anti-IL-6 (human,

(13) Atorvastatin alleviates oxidative damage by activating the nuclear factor erythroid 2-related factor 2 pathway after brain ischemia in miceAnnals of translational medicineOctober 1, 2022Fan J, Zhang X, Chen R.(ab207612; 1:2,000; Abcam), HO-1 (ab52947; 1:1,000; Abcam), NQO1 (ab80588; 1:800; Abcam), β-tubulin (ab179513; 1:2,000; Abcam), LC3B (ab48394; 1:2,000; Abcam), P62 (arg57285; 1:1,000; Arigo), GAPDH (ab9485; 1:5,000;

(14) TauLUM, an in vivo Drosophila sensor of tau multimerization, identifies neuroprotective interventions in tauopathyCell Reports MethodsSeptember 19, 2022Simon A. Levy, Gabrielle Zuniga, Elias M. Gonzalez, David Butler, Sally Temple, Bess FrostAB_528487 cTau,Dako,A0024; RRID: AB_10013724 EGFP,Invitrogen,CAB4211; RRID: AB_10709851 β-tubulin,Abcam,Ab179513 Alexa Fluor 488 anti-rabbit,Invitrogen,A11008; RRID: AB_143165 Alexa Fluor 555 anti-mouse,Invitrogen,A28180;

(15) Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseasesScience AdvancesSeptember 16, 2022Tao Wan, Jiafeng Zhong, Qi Pan, Tianhua Zhou, Yuan Ping, Xiangrui LiuAnti–glyceraldehyde-3-phosphate dehydrogenase (1:1000; ab8245) and anti-tubulin antibodies (1:1000; ab179513) were obtained from Abcam (Cambridge, UK). Cells and animals Huh-7 and AML-12 cells were purchased

(16) MiR-4739 inhibits the malignant behavior of esophageal squamous cell carcinoma cells via the homeobox C10/vascular endothelial growth factor A/phosphatidylinositol 3-kinase/AKT pathwayBioengineeredJune 22, 2022Kaiqin Wu, Zhenchuan Liu, Chenglai Dong, Shaorui Gu, Lei Li, Wenli Wang, Yongxin Zhou(ab214424) (1:1,000 dilution; Abcam), anti-GAPDH (ab181602) (1:5,000 dilution; Abcam), anti-beta tubulin (ab179513) (1:10,000 dilution; Abcam), anti-PI3-kinase p85-alpha (T40115S) (1:1,000 dilution) (Abmart, Shanghai,

(17) Analysis of extracellular vesicle DNA at the single-vesicle level by nano-flow cytometryJournal of extracellular vesiclesApril 1, 2022Liu H, Tian Y, Xue C, Niu Q, Chen C, Yan X.rabbit anti‐human calnexin antibody (Abcam, ab213243), rabbit anti‐human beta‐tubulin antibody (Abcam, ab179513) or primary rabbit anti‐human histone H3 antibody (Cell Signaling Technology, 4499T) overnight at 4°C.

(18) Erebosis, a new cell death mechanism during homeostatic turnover of gut enterocytesPLoS biologyApril 1, 2022Ciesielski HM, Nishida H, Takano T, Fukuhara A, Otani T, Ikegawa Y, Okada M, Nishimura T, Furuse M, Yoo SK.#9578), rabbit-anti-Fibrillarin antibody (1:100, Abcam, #ab5821), rabbit-anti-beta Tubulin antibody (1:100, Abcam, #ab179513), Alexa Fluor phalloidin (1:100, A22287), Alexa Fluor GFP-Booster (1:200, Chromotek, gb2AF488-50) Alexa

(19) Ischemic postconditioning protects nonculprit coronary arteries against ischemia-reperfusion injury via downregulating miR-92a, miR-328 and miR-494Aging (Albany NY)March 31, 2022Jian Wang, Wu Wang, Chengying Yan, Tianzhen Wangno. ab124734), anti-Cx43 (1:1000, Abcam, cat. no. ab11370), anti-β-tubulin (1:1000, Abcam, cat. no. ab179513), anti-Bax (1:1000, Abcam, cat. no. ab32503), anti-Bcl-2 (1:1000, Abcam, cat. no. ab196495), anti-cleaved caspase 3

(20) Comparison of axon extension: PTFE versus PLA formed by a 3D printerOpen Life SciencesMarch 31, 2022Naofumi Kawai, Mizuki Bando, Kento Yuasa, Masayuki Shibasakiincubation at 37°C for 30 min and aspirating Blocking One, an anti-beta tubulin antibody (cat. no. ab179513; Abcam) was added as the primary antibody (1:1,000). After overnight incubation at 4°C or incubation

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Anti-beta Tubulin antibody [EPR16774] from Abcam

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Anti-beta Tubulin antibody [EPR16774] from Abcam

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