Anti-Choline Acetyltransferase antibody [EPR16590] ab178850 from Abcam

Product Specs
ItemAnti-Choline Acetyltransferase antibody [EPR16590]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab178850
Quantity10 UL, 40 UL, 100 UL
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Concentration2.205 mg/ml
Conjugate/TagUnconjugated
IsotypeIgG
CloneEPR16590
HostRabbit
Antibody TypePrimary
ClonalityMonoclonal
NCBI Gene AliasesChoAcTase
Research AreaNeuroscience
Gene NameCHAT
ReactivityGuinea pig, Mouse, Rabbit, Rat
TargetCholine Acetyltransferase

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune functionJournal of neuroinflammationJuly 4, 2023Kobori N, Moore AN, Redell JB, Dash PK.from Sigma-Aldrich (St. Louis, MO). Anti-saCas9 (ab203933) and anti-choline acetyltransferase (ChAT, ab178850 and ab221793) antibodies were purchased from Abcam (Cambridge, UK). Anti-red fluorescent protein antibody

(2) SARS-CoV-2 S1 protein causes brain inflammation by reducing intracerebral acetylcholine productioniScienceJune 16, 2023Naomi Oka, Kazuya Shimada, Azusa Ishii, Nobuyuki Kobayashi, Kazuhiro Kondoanti-Doublecortin,Abcam,Cat# ab18723; RRID: AB_732011 Rabbit polyclonal anti- Choline Acetyltransferase,Abcam,Cat# ab178850; RRID: AB_2721842 "Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor

(3) TDP-43-stratified single-cell proteomic profiling of postmortem human spinal motor neurons reveals protein dynamics in amyotrophic lateral sclerosisbioRxivJune 15, 2023Amanda J. Guise, Santosh A. Misal, Richard Carson, Hannah Boekweg, Daisha Van Der Van Der Watt, Thy Truong, Yiran Liang, Jen-Hwa Chu, Nora C. Welsh, Jake Gagnon, et al.(L95A-42, Biogen) at 1:40,000 and a rabbit monoclonal antibody targeting ChAT (Clone: EPR16590, Abcam, Ref. ab178850) at 1:3,000. Bound anti-TDP-43 and anti-ChAT primary antibodies were detected using an AP-conjugated-anti-mouse

(4) In vivo genome editing using novel AAV-PHP variants rescues motor function deficits and extends survival in a SOD1-ALS mouse modelGene TherapyMay 1, 2023Yi A. Chen, Mark W. Kankel, Sam Hana, Shukkwan Kelly Lau, Maria I. Zavodszky, Olivia McKissick, Nicole Mastrangelo, Jessica Dion, Bin Wang, Daniel Ferretti, et al.Primary antibody (rabbit monoclonal anti-choline acetyltransferase, clone EPR16590, Abcam, Cat. No. ab178850) was applied at the final concentration of 2 µg/mL for 60 min at ambient temperature. Goat anti-rabbit

(5) Cholinergic and noradrenergic axonal activity contains a behavioral-state signal that is coordinated across the dorsal cortexeLifeApril 27, 2023Lindsay Collins, John Francis, Brett Emanuel, David A McCormickincubated for 48–72 hr at 4°C in primary antibodies: anti-choline acetyltransferase 1:300 (Abcam Cat# ab178850, RRID:AB_2721842), anti-tyrosine hydroxylase 1:300 (Abcam Cat# ab137869, RRID:AB_2801410), and/or anti-GFP

(6) Polypyrimidine tract binding protein knockdown reverses depression-like behaviors and cognition impairment in mice with lesioned cholinergic neuronsFrontiers in Aging NeuroscienceApril 27, 2023Yiying Zhou, Ke Zhang, Fangmin Wang, Jiali Chen, Shanshan Chen, Manqing Wu, Miaojun Lai, Yisheng Zhang, Wenhua Zhoucontaining rabbit polyclonal NeuN (1:500; 94,403; CST, Danvers, MA, United States) and ChAT (1:1000; ab178850; Abcam, Cambridge, United Kingdom) in blocking buffer for 12 h at 4°C. After three times with PBS washing,

(7) In vivo imaging of axonal transport in peripheral nerves of rodent forelimbsNeuronal SignalingMarch 31, 2023Qiuhan Lang, Giampietro Schiavo, James N. Sleigh[41]. The following primary antibodies were used: anti-choline acetyltransferase (ChAT, rabbit, Abcam, ab178850/RRID: AB_2721842, 1:250), anti-neurofilament (2H3, mouse, Developmental Studies Hybridoma Bank [DSHB],

(8) Immunohistochemical characteristics of local sites that trigger atrial arrhythmias in response to high-frequency stimulationEP EuropaceFebruary 16, 2023Min-young Kim, James Nesbitt, Simos Koutsoftidis, Joseph Brook, David S Pitcher, Chris D Cantwell, Balvinder Handa, Catherine Jenkins, Charles Houston, Stephen Rothery, et al.hydroxylase (TH) (Abcam ab76442), and rabbit monoclonal antibody to choline acetyltransferase (ChAT) (Abcam ab178850), in separate sections. After washing, secondary antibodies conjugated with fluorescent markers were

(9) Synaptogenic gene therapy with FGF22 improves circuit plasticity and functional recovery following spinal cord injuryEMBO molecular medicineFebruary 8, 2023Aljović A, Jacobi A, Marcantoni M, Kagerer F, Loy K, Kendirli A, Bräutigam J, Fabbio L, Van Steenbergen V, Pleśniar K, et al.antibodies. In order to label moto neurons, we co‐stained with rabbit anti‐ChAT antibody (1:100; Abcam, #ab178850) and NeuroTrace435 (1:200; ThermoFisher, #N21479). Primary antibodies were diluted in 0.3% Triton 20

(10) Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual GangliaBrain SciencesFebruary 3, 2023Md Nabiul Islam, Yoshinori Sakurai, Yurie Hiwaki, Abu Md Mamun Tarif, Marya Afrin, Mirza Mienur Meher, Kanako Nozaki, Koh Hei Masumoto, Akie Yanai, Mir Rubayet Jahan, et al.UK",1:5000,[10] Rabbit Polyclonal anti-ChAT,Synthetic peptide within Pig Choline Acetyltransferase aa 150–250,"Cat# ab178850, RRID: AB_2721842","Abcam, Cambridge, UK",1:5000,[11] Goat Polyclonal anti-ChAT,Human placental ChAT,"Cat#

(11) Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axisNature communicationsJanuary 20, 2023Pei F, Ma L, Jing J, Feng J, Yuan Y, Guo T, Han X, Ho TV, Lei J, He J, et al.Tyrosine Hydroxylase (TH) (1:100, Millipore, AB152), Choline Acetyltransferase (ChAT) (1:100, Abcam, ab178850), FGF1 (1:100, Abcam, ab169748), Advillin (P92) (1:100, Abcam, ab72210), β galactosidase(β-gal) (1:100,

(12) Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injuryFrontiers in NeuroscienceDecember 15, 2022Chuan Qin, Yi Liu, Pei-Pei Xu, Xin Zhang, Zuliyaer Talifu, Jia-Yi Liu, Ying-Li Jing, Fan Bai, Li-Xi Zhao, Yan Yu, et al.(ab177487, 1:500, Abcam, Cambridge, MA, USA), a neuronal marker; anti-choline acetyltransferase (ChAt, ab178850, 1:2000, Abcam, Cambridge, MA, USA), a marker for motor neurons; and anti-GFP (ab13970, 1:500, Abcam,

(13) Loss of Retinogeniculate Synaptic Function in the DBA/2J Mouse Model of GlaucomaeneuroDecember 14, 2022Jennie C. Smith, Kevin Yang Zhang, Asia Sladek, Jennifer Thompson, Elizabeth R. Bierlein, Ashish Bhandari, Matthew J. Van Hooksolution containing a rabbit-anti-choline acetyltransferase (ChAT) monoclonal antibody (1:1000, Abcam ab178850, RRID:AB_2721842) and a guinea pig-anti-NeuN polyclonal antibody (1:500, Millipore ABN90, RRID:AB_11205592).

(14) Single-Cell Transcriptional Profiling Reveals Low-Level Tragus Stimulation Improves Sepsis-Induced Myocardial Dysfunction by Promoting M2 Macrophage PolarizationOxidative Medicine and Cellular LongevityOctober 15, 2022Yufan Yang, Longlong Xie, Yinghui Peng, Haipeng Yan, Jiaotian Huang, Zhenghui Xiao, Xiulan Lutragus tissues were stained with antibodies targeting choline acetyltransferase (ChAT) (ChAT; 1 : 200, ab178850, Abcam). 2.7. Western Blotting The heart of mice from each group was used for western blotting. Briefly,

(15) Dramatic impacts on brain pathology, anxiety, and cognitive function in the knock-in APPNL-G-F mouse model of Alzheimer disease following long-term voluntary exerciseAlzheimer's Research & TherapySeptember 30, 2022Jogender Mehla, Scott H. Deibel, Hadil Karem, Shakhawat Hossain, Sean G. Lacoursiere, Robert J. Sutherland, Majid H. Mohajerani, Robert J. McDonaldchamber on the shaker. The following primary antibodies were used: rabbit anti-ChAT (monoclonal, Abcam, ab178850, 1:5000); anti-IBA-1 (Rabbit, SAF4318, 019-19741, Wako). Following incubation, three 10-min washes were

(16) Improvement of Cognitive Function in Ovariectomized Rats by Human Neural Stem Cells Overexpressing Choline Acetyltransferase via Secretion of NGF and BDNFInternational journal of molecular sciencesMay 16, 2022Yoon EJ, Choi Y, Park D.Signal,#5125,1:1000,Rb ER α,Abcam,Ab66102,1:100,Ms ER β,Abcam,Ab288,1:100,Ms CHT1,Abcam,Ab154186,1:1000,Rb ChAT,Abcam,Ab178850,1:1000,Rb VAChT,Synaptic systems,#139103,1:500,Rb List of antibodies used in the current study.

(17) The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal developmentNature geneticsMay 1, 2022Gu T, Hao D, Woo J, Huang TW, Guo L, Lin X, Guzman AG, Tovy A, Rosas C, Jeong M, et al.(1:250, R&D systems, AF3075), mouse anti-OLIG2 (1:200, Sigma, MABN50), rabbit anti-ChAT (1:500, Abcam, ab178850), rabbit anti-DNMT3A (1:250, Cell signaling, 3598S), goat anti-mouse IgG, AF488 (1:500, Invitrogen,

(18) Gain control by sparse, ultra-slow glycinergic synapsesCell ReportsFebruary 22, 2022Varsha Jain et al.labeled the tagged ACh 3.0 sensor, and a rabbit anti-Choline Acetyltransferase antibody (1:500; Abcam ab178850) labeled the ON and OFF SACs. Retinas were incubated in the secondary antibodies donkey anti-goat Alexa

(19) TGFβ-induced expression of long noncoding lincRNA Platr18 controls breast cancer axonogenesisLife science allianceFebruary 1, 2022Grelet S, Fréreux C, Obellianne C, Noguchi K, Howley BV, Dalton AC, Howe PH.hydroxylase (Cat. No. ab75875, 1:100 dilution) purchased from Abcam and acetylcholine transferase (Cat. No. ab178850, 1:500 dilution) purchased from Abcam or TRPV1 (Cat. No. ACC-030, 1:500 dilution) purchased from Alomone labs

(20) The Untouchable Ventral Nucleus of the Trapezoid Body: Preservation of a Nucleus in an Animal Model of Autism Spectrum DisorderFrontiers in Integrative NeuroscienceSeptember 29, 2021Yusra Mansour, Randy J. Kuleszafor ChAT were incubated in primary antisera (rabbit anti-ChAT, 1:1000 with 1% NHS; Abcam, catalog #: ab178850) overnight, rinsed in PBS and incubated for 2 h in goat anti-rabbit Dylight 488 (1:100; Vector Labs).

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Anti-Choline Acetyltransferase antibody [EPR16590] from Abcam

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Anti-Choline Acetyltransferase antibody [EPR16590] from Abcam

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