Fig 1: Circ_0075825 promotes malignant phenotypes of GC cells by regulating miR-432-5p / SOX9 axis. (A) NUGC4 cells with circ_0075825 overexpression were transfected with miR-432-5p mimics and SOX9 overexpression vector, and the protein expression of SOX9 in GC cells was detected by western blot assay. (B) CCK-8 assay was used to detect the proliferation of NUGC4 cells after the transfection. (C-D) Transwell assay was used to detect the migration and invasion of NUGC4 cells after the transfection. (E) Flow cytometry assay was used to detect the apoptosis of NUGC4 cells after the transfection. **p < 0.01, and ***p < 0.001.
Fig 2: miR-30a could target SOX9. A TargetScan, DIANA, miRSearch and miRDB databases predicted the target genes of miR-30a, and the predicted results were compared with the DEGs on GSE26712 and GSE4122; B, C Differential expression of SOX9 and NT5E on GSE26712 and GSE4122; D TargetScan software predicted the potential binding sites of miR-30a to SOX9; E Luciferase activity detected the targeting relationship between miR-30a and SOX9; vs the mimic-NC cells, **p < 0.01; F-G: Relative SOX9 expression in CD133+ OCSCs detected by RT-qPCR and Western blot analysis, vs mimic-NC cells, *p < 0.05; H. Relative expression of SOX9 and miR-30a in CD133+ OCSCs after silencing LINC00115 detected by RT-qPCR. Compared with the Ctrl group, *p < 0.05, **p < 0.01. Data in panels E–G were analyzed using the unpaired t test, while data in panel H were analyzed using one-way ANOVA and Tukey’s multiple comparison test. The experiment was performed in triplicate and data were expressed as means ± standard deviation
Fig 3: Proliferation of hair follicle stem cells after BMSC and MSC-CM treatment (A) Krt15 (red fluorescence) was observed in the Bu region and Ki67 (green fluorescence) was observed in the bulb area and infundibulum of hair follicle at 0, 7, 10 and 15 days (B) Sox9 fluorescence (red fluorescence) in the Bu and ORS region and Ki67 (green fluorescence) in the bulb area and infundibulum at 0, 7, 10 and 15 days. Nuclei were stained with DAPI (blue). The dashed line delineates the hair follicle structure. The yellow triangles show the expression site of the bulge (Bu) region and white triangles show the expression site of outer root sheaths (ORS). The bulb areas are indicated by yellow arrows and infundibula are indicated by white arrows. Scale bar: 200 µm.
Fig 4: Point-plots of Taqman-based gene expression of GS-5 GSCs after stable transduction with a non-targeting shRNA (shCtrl) or specific shRNA targeting CHRDL1 (shCHRDL1) and measurement of (A) CHRDL1, (B) OLIG2, (C) SOX2, (D) SOX9, (E) ZEB1, (F) ZEB2, (G) CD44, (H) NES, (I) GFAP, (J) NEFL, (K) MAP2 and (L) RBFOX3 expression. The data are the summary of three experiments performed in triplicates. * p < 0.05; *** p < 0.001; **** p < 0.0001; ns: not significant; unpaired t test with Welch’s correction; white dot: shCtrl, red dot: shCHRDL1.
Fig 5: Nucleus pulposus-related gene expression levels of ADSCs. Gene expression levels of Acan (A), Col-I (B), Sox9 (C), and Col-II (D) were measured at 7 and 14 days. Verification of Western blot for NP-related gene expression at 7 days is shown in (E–I). Data represented mean ± SD (n = 5); *p < 0.05, vs. 0% group; # p < 0.05, vs. 0.2% group.
Supplier Page from Abcam for Anti-SOX9 antibody [EPR14335-78]