Fig 1: Effects of two types of astrocyte activation on anoxic neurons. The anoxic neurons were co-cultured with the two groups of astrocytes separated by magnetic particle label. TUNEL (a) and FJC (b) were used to detect the apoptosis and denaturation of neurons, with scale bar of 50 µm. d The statistical results of TUNEL showed that the anoxic neurons had more apoptosis than control group (P < 0.001); the iNOS(+) group had more apoptosis than the anoxic neurons group (P < 0.01), while the iNOS(-) group had less apoptosis than the anoxic neurons group (P < 0.05). e The denaturation of each group was parallel to apoptosis. c Immunofluorescence staining was used to observe the morphological structure of neurons. The structure of the synapse was damaged in the anoxic neuron group. Neurons in iNOS(+) group were relative integrity, while the neurons in iNOS(-) group were more seriously damaged, scale bar 10 µm. Antibody: mouse anti-beta-III-Tubulin (1:300, Cat#ab78078, Abcam), anti-mouse Alex 488 conjugated secondary antibody (1:200, Cat#ab150117, Abcam)