Fig 1: Evolutionary conserved expression of SOX21 in human airway epithelium.(A) Immunofluorescence on human fetal lung sections post-conceptional week (PCW) 13, 16.5, and 17. Proximal–distal patterning of human developing airways is analyzed with SOX9 (red) and SOX2 (blue) (left). In the small developing airways (left), no expression of SOX21 (green) was observed. On the right, co-staining of SOX2 (blue), SOX21 (green), and TP63 (red). The closed white arrowheads show cells co-expressing TP63 and SOX21. Scale bar = 50 µm. (B) Top and middle row: Immunofluorescence analysis of fetal lung organoids shows SOX2 (grey), SOX9 (red), and SOX21 (green) positive cells. White closed triangles (?) show cells positive for SOX2 and SOX9, open triangles (w) show cells positive for SOX2, SOX21, and SOX9. Arrows (g) show cells positive for SOX21 and SOX2. Bottom row: Immunofluorescence analysis of SOX2 (grey), TP63 (red), and SOX21 (green) in fetal lung organoids. White closed arrowheads (?) show cells positive for SOX2, TP63, and SOX21. Scale bar = 50 µm. (C) Fetal lung organoids differentiated to airway epithelium, as shown by the presence of ciliated cells (TUBIV; left) or basal cells (TP63, right), have an abundant expression of SOX21 (green). Scale bar = 50 µm. (D) Immunofluorescence analysis of sections of human adult bronchi shows co-localization of SOX2 (red) and SOX21 (green) throughout the epithelium (1, top row). SOX21 (green) is expressed in luminal (KRT8; grey) and basal (KRT5; red) cells (2, middle row). SOX21 is expressed in basal (TP63; red) and ciliated (FOXJ1; grey) and is high expressed in cells absent of TP63 and FOXJ1(g) (3, bottom row). Scale bar = 25 µm.
Fig 2: Immunohistological stain of Sox9 in 14 normal brain tissues and 86 glioma tissues (p < 0.01). (A) Western blot of Sox9 in glioma tissues (C) and paired adjacent tissues (P) of five patients. (B) Analysis of the expression of Sox9 and the prognosis of glioma patients from CGGA database (C) and TCGA database (D). Value of p < 0.01 (**) was considered statistically significant.
Fig 3: H2O2 increased USP7 expression and caused damage in rat chondrocytes. (A) Representative immunohistochemical staining for collagen II and SOX9 in rat chondrocytes. Rat chondrocytes were treated with different concentrations of H2O2; (B,C) ROS production, (D) cell proliferation, and (E) USP7 expression was measured by flow cytometry, CCK8, and Western blot, respectively. Scale bar, 100 µm ***p < 0.001 vs. 0 µM.
Fig 4: Postnatal Gli1+ cells are spatially located at the superficial layers of the cartilage and chondro-osseous junction. (A) Histological analysis of mandibular condyles from wild type (WT) mice at PN 3.5 days, 1 week, 2 weeks, and 1 month. (B) Sox9 and ß-gal double-immunostaining of condyles from Gli1-LacZ mice at PN 3.5 days, 1 week, 2 weeks, and 1 month. The lower panel shows the high-magnification images of the white box insets in the upper panel. The yellow dotted line in (A) shows the chondro-osseous junction. Arrows in (B) show Gli1+ cells at the chondro-osseous junction. SF, superficial layer; PM, polymorphic zone; FC, flattened chondrocyte zone; HC, hypertrophic zone. n = 3 mice/group. Scale bars, 100 µm.
Fig 5: TAMs promote tumor metastasis via the TGF-ß/SOX9 axis in non-small cell lung cancerTGF-ß secreted by TAMs activated the C-Jun and SMAD3 pathway in lung cancer cells, which increased SOX9 expression and promoted EMT in lung cancer cells. EMT promotes proliferation and metastasis in lung cancer cells.
Supplier Page from Abcam for Anti-SOX9 antibody [EPR14335]