Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537 from Abcam

Product Specs
ItemAnti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab181537
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
IsotypeIgG
CloneEPR17034
HostRabbit
Antibody TypePrimary
ClonalityMonoclonal
NCBI Gene AliasesHMG20
ModificationsLinkage-Specific-K27
Gene NameUBC
ReactivityHuman, Mouse, Rat, Recombinant fragment
TargetUbiquitin

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) USP10 Regulates ZEB1 Ubiquitination and Protein Stability to Inhibit ZEB1-Mediated Colorectal Cancer MetastasisMolecular cancer research : MCRJune 1, 2023Sun L, Yu J, Guinney J, Qin B, Sinicrope FA.K48-linkage ubiquitination (Abcam Cat# ab140601, RRID:AB_2783797) and K27 linkage ubiquitination (Abcam Cat# ab181537, RRID:AB_2713902) were used, as was as anti-HA antibody (Proteintech Cat# 51064-2-AP, RRID:AB_11042321).

(2) Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3Nature communicationsSeptember 19, 2022Zhang X, Xiong T, Gao L, Wang Y, Liu L, Tian T, Shi Y, Zhang J, Zhao Z, Lu D, et al.anti-phospho-Erk1/2 (9101, CST), rabbit anti-phospho-JNK (4668, CST), rabbit anti-GFP (2956, CST), rabbit anti-K27 (ab181537, Abcam), rabbit anti-K48 (8081, CST), rabbit anti-K63 (5621, CST), rabbit anti-cIAP1 (ab2399, Abcam),

(3) Targeting HECTD3-IKKα axis inhibits inflammation-related metastasisSignal transduction and targeted therapyAugust 3, 2022Li F, Liang H, You H, Xiao J, Xia H, Chen X, Huang M, Cheng Z, Yang C, Liu W, et al.antibodies were from Sigma-Aldrich. The anti-Ub (04–263) Ab was from Millipore(MA, USA). The anti-K27 Ub Ab (ab181537) was from Abcam (MA, USA). The anti-tubulin Ab (11224-1-AP) was from Proteintech (IL, USA). The anti-GFP

(4) K27-linked ubiquitylation promotes p97 substrate processing and is essential for cell proliferationThe EMBO journalMay 2, 2022Shearer RF, Typas D, Coscia F, Schovsbo S, Kruse T, Mund A, Mailand N.(11867423001, Roche (1:1,000), RRID: AB_390918), FLAG (F1804, Sigma (1:5,000), RRID: AB_262044), K27‐linked Ub (ab181537, Abcam (1:5,000), RRID: AB_2713902), NPL4 (13489, Cell Signaling (1:1,000), RRID: AB_2798232), p47 (NBP2‐13677,

(5) Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent MitophagyMolecular & cellular proteomics : MCPFebruary 1, 2022Zittlau KI, Lechado-Terradas A, Nalpas N, Geisler S, Kahle PJ, Macek B.1:10000, Abnova (#H00055669-M04); 6HIS, 1:10000, Invitrogen (#HIS.H8); K27-ubiquitin, 1:50000, Abcam (#ab181537); Total Ubiquitin, 1:30000, Millipore (#MAB1510); pS65-ubiquitin, 1:1000, Millipore (#ABS1513); Parkin,

(6) The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradationPLoS pathogensFebruary 1, 2021Zeng Y, Xu S, Wei Y, Zhang X, Wang Q, Jia Y, Wang W, Han L, Chen Z, Wang Z, et al.anti-GAPDH (ab181602), anti-ubiquitin (K63) (ab179434), anti-ubiquitin(K48) (ab140601), anti-ubiquitin(K27) (ab181537) antibodies (Abcam); anti-ubiquitin (WT) antibody (Santa Cruz, sc-8017); anti-LC3(PM036) and anti-ATG7

(7) RNF208, an estrogen-inducible E3 ligase, targets soluble Vimentin to suppress metastasis in triple-negative breast cancersNature CommunicationsDecember 20, 2019Kyoungwha Pang et al.Healthcare). Rabbit antibody against RNF208 (ab175506, dilution 1:1000) and Ubiquitin (linkage-specific K27) (ab181537, dilution 1:1000) and mouse antibody against Vimentin [V9] (ab8069, dilution 1:1000), Vimentin [RV202]

(8) RNF34 functions in immunity and selective mitophagy by targeting MAVS for autophagic degradationThe EMBO journalJuly 15, 2019He X, Zhu Y, Zhang Y, Geng Y, Gong J, Geng J, Zhang P, Zhang X, Liu N, Peng Y, et al.(Sigma‐Aldrich, A5598), anti‐HA (Sigma‐Aldrich, H9658), anti‐Ub (Epitomics, 6652‐1), anti‐Ub‐27 (Abcam, ab181537), anti‐Ub‐63 (Epitomics, 8070‐1), anti‐TBK1 (Santa Cruz, sc‐166583, sc‐52957), anti‐TBK1 (pSer172) (ab109272),

(9) USP19 Inhibits TNF-α- and IL-1β-Triggered NF-κB Activation by Deubiquitinating TAK1Journal of immunology (Baltimore, Md. : 1950)July 1, 2019Lei CQ, Wu X, Zhong X, Jiang L, Zhong B, Shu HB.and 8409; Santa Cruz Biotechnology); ubiquitin (ab7254; Abcam); K27-linkage specific polyubiquitin (ab181537; Abcam); K48-linkage specific polyubiquitin (ab140601; Abcam); K63-linkage specific polyubiquitin (ab179434;

(10) K27-linked ubiquitination of BRAF by ITCH engages cytokine response to maintain MEK-ERK signalingNature communicationsApril 23, 2019Yin Q, Han T, Fang B, Zhang G, Zhang C, Roberts ER, Izumi V, Zheng M, Jiang S, Yin X, et al.anti-rabbit secondary antibody (A-4914, 1:3000) were purchased from Sigma. Anti-K27-linkage ubiquitin (ab181537, 1:2000) was purchased from Abcam. Monoclonal anti-HA antibody (MMS-101P, 1:2000) was purchased from

(11) The deubiquitinase MYSM1 dampens NOD2-mediated inflammation and tissue damage by inactivating the RIP2 complexNature CommunicationsDecember 1, 2018Swarupa Panda, Nelson O. GekaraAnti-MYSM1 (ab193081, 1:3000), anti-HOIP (ab85294, 1:500), anti-IKKα/β (ab178870, 1:1000) and anti-K27 (ab181537, 1:2000) were purchased from Abcam. Anti-TAB2 (MA514818,1:500), RBC-8c5 (35-5931-80, 1:500) NuPAGE™

(12) GBPs Inhibit Motility of Shigella flexneri but Are Targeted for Degradation by the Bacterial Ubiquitin Ligase IpaH9.8Cell host & microbeOctober 11, 2017Wandel MP, Pathe C, Werner EI, Ellison CJ, Boyle KB, von der Malsburg A, Rohde J, Randow F.monoclonal,Santa Cruz Biotechnology,Cat# sc-271568; RRID: AB_10655677 Ubiquitin K27 rabbit monoclonal,Abcam,Cat# ab181537; RRID: AB_2713902 Flag-tag (M2) mouse monoclonal,Sigma,Cat# F1804; RRID: AB_262044 β-actin rabbit

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Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] from Abcam

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Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] from Abcam

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