Fig 1: Human TE cell differentiation during peri-implantation.(A) Cell lineage identification of S1, S2, and S3 TEs (first three columns). Integrated analysis of S2 TE in this study and E6 and E7 TE from the previous study [Petropoulos et al. (5)] (last column). (B) Unsupervised hierarchical clustering of highly variable genes in TE cells of three stages and the corresponding GO analysis. (C) Pseudotime analysis of TE cells of three stages. The arrows show the developmental trajectory. Colors represent corresponding cell types. (D) Dot plots show relative gene expression levels [log2 (TPM/10 + 1), average levels ± SEMs] of specifically expressed genes for each TE cell type. (E) Immunofluorescence images of the TE-specific gene CYP26A1 in S1 embryos, MTE-specific gene HAND1, and the PTE-specific gene GCM1 in S2 embryos. ICM is marked by an *. Scale bar, 100 µm.