Fig 1: Analysis of apoptosis and cellular senescence markers induced by LEE011 in leukemia cells. a Hoechst 33,342 staining analysis showing cells treated with LEE011 at 2 and 5 µM, demonstrates an increase in cells with DNA fragmentation and abnormal nuclear structure following 48-h LEE011 treatment. b The number of cells with abnormal nuclear structure was calculated in each group. For MV4-11 cells, the proportion with abnormal nuclear structure in the 5-µM treatment group was 28.93 ± 6.50% vs. DMSO group 5.60 ± 2.29%, P = 0.0016; for HL-60 cells, the respective values were 25.60 ± 3.30 vs. 3.27 ± 1.84%, P = 0.0013. **P < 0.01. c Western blotting of molecular markers of apoptosis, including PARP, caspase-3 and caspase-9, and molecular markers of cellular senescence, p16INK4a and p21Waf1/Cip1. Upregulation of p16INK4a was observed in the LEE011-treatment groups for both MV4-11 and HL-60 cells
Fig 2: (a) A slide prepared by the LBP method, showing a cluster of small cell type HSIL cells that are easily overlooked (Papanicolaou stain, ×400). (b) A slide from the same patient immunostained with p16, showing that the HSIL cells are easy to interpret because of the obviously positive nucleus. (c) A slide prepared by the LBP method, showing some scattered HSIL cells that are easily overlooked (Papanicolaou stain, ×400). (d) A slide from the same patient immunostained with the p16, showing that the HSIL cells are easy to interpret because the nucleus is obviously positive. (e) A slide prepared by the LBP method, showing a cluster of HCGs-type HSIL cells that are easily misinterpreted (Papanicolaou stain, ×400). (f) A slide from the same patient immunostained with the p16, showing that the HSIL cells are easy to interpret because the nucleus is obviously positive. (g) A slide prepared by the LBP method, showing a cluster of metaplastic-type HSIL cells that are easily misinterpreted (Papanicolaou stain, ×400). (h) A slide from the same patient immunostained with the p16, showing that the HSIL cells are easy to interpret because the nucleus is obviously positive.
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