Purified anti-Ubiquitin 646302 from BioLegend

Product Specs
ItemPurified anti-Ubiquitin
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number646302
NCBI Gene AliasesCEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80, eS31
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
HostMouse
Conjugate/TagUnconjugated
IsotypeIgG1, Kappa
ApplicationsWestern Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Quantity100 µg
ReactivityAll species
CloneP4D1
Antibody TypePrimary
NCBI Full Gene Nameribosomal protein S27a
Concentration0.50 mg/mL
ImmunogenDenatured glutaraldehyde cross-linked ubiquitin IgG complex
ClonalityMonoclonal
TargetUbiquitin

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Discovery of XL01126: A Potent, Fast, Cooperative, Selective, Orally Bioavailable, and Blood–Brain Barrier Penetrant PROTAC Degrader of Leucine-Rich Repeat Kinase 2Journal of the American Chemical SocietySeptember 21, 2022Xingui Liu et al.(Cat# MAB1536) and was diluted to 1:1000. Mouse monoclonal Ubiquitin was purchased from Biolegend (Cat# 646302) and was diluted to 1:1000. Rabbit polyclonal PDE6D antibody was purchased from Novus Biologicals and

(2) Norovirus Intestinal Infection and Lewy Body Disease in an Older Patient with Acute Cognitive ImpairmentInternational Journal of Molecular SciencesJuly 29, 2022Manuel Moreno-Valladares, Veronica Moncho-Amor, Iraide Bernal-Simon, Eñaut Agirre-Iturrioz, María Álvarez-Satta, Ander Matheu(Millipore, Ref.: MAB377, Clone: A60), neurofilament (Roche, Ref.: 760-2661), ubiquitin (BioLegend, Ref.: 646301), Iba1 (Santa Cruz Biotechnology, Ref.: sc-32725), GFAP (Cell Marque, Ref.: 760-4345), and p38 MAPK

(3) ER-stress promotes VHL-independent degradation of hypoxia-inducible factors via FBXW1A/βTrCPRedox biologyApril 1, 2022Mennerich D, Kubaichuk K, Raza GS, Fuhrmann DC, Herzig KH, Brüne B, Kietzmann T.(#sc-166 659; 1:1000; Santa Cruz), GSK3β (3D10) (#9832; 1:1000; Cell signaling), Ubiquitin (P4D1) (#646302; 1:3000; BioLegend), V5 (#R960-25; 1:5000; Invitrogen), β-actin (#A5316; 1:5000; Sigma Aldrich) and

(4) TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell lossActa neuropathologica communicationsMarch 14, 2022Feng T, Luan L, Katz II, Ullah M, Van Deerlin VM, Trojanowski JQ, Lee EB, Hu F.anti-GAPDH (Proteintech Group, 60004-1-Ig), rabbit anti-p62 (MBL, PM045), mouse anti-Ubiquitin (BioLegend, 646302), rabbit anti-TDP-43 (Proteintech Group, 12892-1-AP), mouse anti-TDP43 (R&D Systems, MAB7778), rabbit

(5) Investigation of energy metabolic dynamism in hyperthermia-resistant ovarian and uterine cancer cells under heat stressScientific ReportsDecember 1, 2021Taisei Kanamori, Natumi Miyazaki, Shigeki Aoki, Kousei Ito, Akihiro Hisaka, Hiroto Hatakeyamaab137791, Abcam, Cambridge, UK), anti-STUB1 (1:10,000, ab134064, Abcam, Cambridge, UK), anti-Ub (1:3000, # 646302, Biolegend, San Diego, CA, USA), and ββ-actin (1:10,000; Sigma-Aldrich, St. Louis, MO, USA). Plasmid

(6) Pharmacological rescue of impaired mitophagy in Parkinson's disease-related LRRK2 G2019S knock-in miceeLifeAugust 3, 2021Singh F, Prescott AR, Rosewell P, Ball G, Reith AD, Ganley IG.(1/1000, Cell Signalling Technology, #62802S), Ubiquitin (P4D1) mouse monoclonal (1/1000, BioLegend, 646302), Parkin mouse monoclonal (1/2000, Santa-Cruz Biotechnology, sc-32282), α-Tubulin (11H10) Rabbit monoclonal

(7) TRIM44 mediated p62 deubiquitination enhances DNA damage repair by increasing nuclear FLNA and 53BP1 expressionOncogeneAugust 1, 2021Lyu L, Lin TC, McCarty N.polyclonal (11511-1-AP) antibody was purchased from the Proteintech Group (Chicago, IL, USA). An anti-Ub (646301) antibody was purchased from Biolegend (San Diego, CA, USA). Anti-mCherry (PA5-34974) antibody was purchased

(8) Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanismsThe Biochemical journalFebruary 12, 2021Malik AU, Karapetsas A, Nirujogi RS, Mathea S, Chatterjee D, Pal P, Lis P, Taylor M, Purlyte E, Gourlay R, et al.(#4806, CST), rabbit monoclonal pP38 (Thr180, Tyr182) (#4631, CST), mouse monoclonal anti-Total Ub (#646302, BioLegend), mouse monoclonal anti-OPA-1 (#612607, BD Biosciences), mouse anti-GAPDH (#sc-32233, Santa Cruz Biotechnology

(9) Deubiquitinating enzyme amino acid profiling reveals a class of ubiquitin esterasesProceedings of the National Academy of Sciences of the United States of AmericaJanuary 26, 2021De Cesare V, Carbajo Lopez D, Mabbitt PD, Fletcher AJ, Soetens M, Antico O, Wood NT, Virdee S.visualized by Coomassie stain or by standard Western blot procedure (anti-ubiquitin antibody, cat. no. 646302, monoclonal, mouse, 0.1 µg/mL; BioLegend). Transient Overexpression of JOSD1 in HEK293 Cells. HEK293 cells

(10) Myocardial infarction affects Cx43 content of extracellular vesicles secreted by cardiomyocytesLife science allianceDecember 1, 2020Martins-Marques T, Ribeiro-Rodrigues T, de Jager SC, Zuzarte M, Ferreira C, Cruz P, Reis L, Baptista R, Gonçalves L, Sluijter JP, et al.against p62 (5114S) and Alix (3A9, 2171S) were obtained from Cell Signaling. Antibodies against Ub (p4D1, 646302) were purchased from BioLegend, Tsg101 (4A10, GTX70255) was from Gentex, and Hsp90 (AC88, 386040) was

(11) Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in miceEMBO reportsOctober 5, 2020Feng T, Mai S, Roscoe JM, Sheng RR, Ullah M, Zhang J, Katz II, Yu H, Xiong W, Hu F.mouse anti‐LC3 (Cell signaling, 83506S), rabbit anti‐p62 (MBL, PM045), mouse anti‐Ubiquitin (BioLegend, 646302), mouse anti‐TDP43 (R&D Systems, MAB7778), rabbit anti‐p‐TDP‐43 S403/S404 (COSMO BIO, CAC‐TIP‐PTD‐P05),

(12) Cooperativity between the Ribosome-Associated Chaperone Ssb/RAC and the Ubiquitin Ligase Ltn1 in Ubiquitination of Nascent PolypeptidesInternational Journal of Molecular SciencesSeptember 17, 2020Arnab Ghosh, Natalia Shcherbikstudy, we used the following antibodies: anti-ubiquitin (clone P4G1, Biolegend, San-Diego, CA, USA, Cat# 646301); anti-FLAG (Sigma, St Louis, MO, USA, Cat# F7425); anti-RPL3 (ScRPL3, Iowa, USA, Developmental Studies

(13) MKRN3 inhibits the reproductive axis through actions in kisspeptin-expressing neuronsThe Journal of clinical investigationAugust 3, 2020Abreu AP, Toro CA, Song YB, Navarro VM, Bosch MA, Eren A, Liang JN, Carroll RS, Latronico AC, Rønnekleiv OK, et al.polyclonal), anti-HA antibody (Abcam ab9110, 1:4000, polyclonal), or anti-ubiquitin antibody (BioLegend 646301, 1:1000, P4D1) overnight at 4°C. Blots were incubated with anti-rabbit secondary antibodies linked to horseradish

(14) DUB3 deubiquitinates and stabilizes NRF2 in chemotherapy resistance of colorectal cancerCell Death & DifferentiationNovember 1, 2019Qi Zhang, Ze-Yan Zhang, Huan Du, Shang-Ze Li, Rongfu Tu, Yi-fan Jia, Zhe Zheng, Xue-Min Song, Run-Lei Du, Xiao-Dong ZhangBiotech), Flag (cat# M185-3L, MBL), Myc (cat# M192-3, MBL), HA (cat# M180-3, MBL), and ubiquitin (cat# 646302, Biolegend); a rabbit monoclonal antibody against NRF2 (D1Z9C) (cat# 12721, Cell Signaling Technology);

(15) Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione DepletionCell metabolismMay 7, 2019Harris IS, Endress JE, Coloff JL, Selfors LM, McBrayer SK, Rosenbluth JM, Takahashi N, Dhakal S, Koduri V, Oser MG, et al.

(16) Tetraspanin CD9 stabilizes gp130 by preventing its ubiquitin-dependent lysosomal degradation to promote STAT3 activation in glioma stem cellsCell death and differentiationJanuary 1, 2017Shi Y, Zhou W, Cheng L, Chen C, Huang Z, Fang X, Wu Q, He Z, Xu S, Lathia JD, et al.(p-Tyr40, #3211, 1:1000, Cell Signaling); anti-BMX (ab59360, 1:1000, Abcam, Cambridge, UK); anti-Ubiquitin (646301, 1:500, Biolegend, San Diego, CA, USA); anti-GFAP (644702, 1:1000, Biolegend); anti-MAP2 (SMI-52 R,

(17) An evolutionarily conserved pathway controls proteasome homeostasisNatureAugust 11, 2016Rousseau A, Bertolotti A.GFP (ab290; Abcam, 1:5000), HA (mHA.11; Covance, 1:2000), TAP (CAB1001; Pierce, 1:1000), ubiquitin (646302 (P4D1); BioLegend, 1:1000), Adc17 (Bertolotti lab; 10 1:1,000), P-T737-Sch9 (Maeda lab; 16 1:5,000),

(18) Differential induction of muscle atrophy pathways in two mouse models of spinal muscular atrophyScientific reportsJune 28, 2016Deguise MO, Boyer JG, McFall ER, Yazdani A, De Repentigny Y, Kothary R.ab9485 - 1:2500 and ab8245 - 1:10000 or 1:12000), LC3B (Abcam: ab48394 - 1:1000), Ubiquitin (BioLegend (646301): Clone P4D1 - 1:1500), P62/Sqstm1 (Abcam: ab56416 - 1:1000), Bnip3 (Abcam: ab10433 - 1:1000), Gabarapl1

(19) Arsenic trioxide disrupts glioma stem cells via promoting PML degradation to inhibit tumor growthOncotargetNovember 10, 2015Zhou W, Cheng L, Shi Y, Ke SQ, Huang Z, Fang X, Chu CW, Xie Q, Bian XW, Rich JN, et al.agarose affinity gel (Sigma-Aldrich, A7470–1ML) and immunoblotted with an anti-ubiquitin (Biolegend, 646301) or anti-c-Myc antibody (Santa Cruz, sc-40). Briefly, cell lysates (300 μg of total protein) were incubated

(20) PP2A blockade inhibits autophagy and causes intraneuronal accumulation of ubiquitinated proteinsNeurobiology of agingMarch 1, 2013Magnaudeix A, Wilson CM, Page G, Bauvy C, Codogno P, Lévêque P, Labrousse F, Corre-Delage M, Yardin C, Terro F.

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Purified anti-Ubiquitin from BioLegend

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Purified anti-Ubiquitin from BioLegend

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