Fig 1: HpBARI administration results in persistent suppression of ST2 in vivo.(A–C) Alternaria allergen and HpBARI was intranasally administered and mice culled 24 hr later, as in Figure 3A–D. Representative ST2 versus lineage bivariate plots (gated on live CD45+ cells) are shown in (A). ST2 geometric mean fluorescence intensity on the surface of ICOS+lineage–CD45+ ILC2s was measured by flow cytometry (B). ST2 transcript was measured by qPCR in lung homogenates (C). PBS, Alt and Alt+HpBARI groups are representative of 2 repeat experiments, each with 3–4 mice per group; HpBARI alone group from a single experiment, n = 3. (D–E) Biotinylated HpBARI was intranasally administered 7 days, 4 days, 2 days or 1 day before Alternaria allergen, or co-administered (co-ad) with it. 24 hr after Alternaria allergen administration, BAL eosinophil (Siglecf+CD11c–) proportions of CD45+ cells (D), and mean fluorescence intensity of ST2 (E) on lung ILC2s (ICOS+Lineage–CD45+) were calculated. Representative results from two repeat experiments, each with three mice per group. Error bars show SEM.