Fig 1: Hypo-CM effectively promoted resolution of inflammation in the lungs of ALI models. (A) Representative images of lung tissues and H&E staining of the lungs. Scale bar: 200 µm. (B) Quantification of lung injury through pathological score evaluation, n = 7. (C) Quantification of total cells in BALF. Cells in BALF were harvested 48 h after LPS injection and then counted. n = 5. (D) Analysis of neutrophils (CD11b+Gr-1+) level in BALF through flow cytometry. n = 3. (E) Quantification of the proportion of alveolar neutrophils in total BALF cells. (F) Analysis of MPO activity in the lungs. Fresh mice lung tissues were collected 48 h after LPS administration and used for measurement of MPO activity. n = 6~8. (G) Analysis of inflammatory factors in the serum through ELISA. Each dot represents data from one animal. n = 3~6. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Statistics for multiple comparisons were obtained from one-way ANOVA. ALI, acute lung injury; i.t., intracheal instillation; MPO, myeloperoxidase; BALF, bronchoalveolar lavage fluid; ns, no statistical significance.