Acetyl-alpha Tubulin (Lys40) Antibody 32-2700 from Thermo Fisher Scientific

Product Specs
ItemAcetyl-alpha Tubulin (Lys40) Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number32-2700
Quantity100 µg
ApplicationsFlow Cytometry, Immunocytochemistry, Western Blot
Conjugate/TagUnconjugated
FormatPBS with 0.1% sodium azide; pH 7.4
ReactivityHuman, Mouse, Rat, Sea urchin
TargetAcetyl-alpha Tubulin (Lys40)
IsotypeIgG2b
Clone6-11B-1
HostMouse
ImmunogenAcetylated alpha-tubulin from the axoneme of sea urchin sperm flagella
NCBI Full Gene Nametubulin, alpha-like 3, tubulin, alpha 1A, tubulin, alpha 1C, tubulin, alpha 1B, tubulin alpha 1a, tubulin alpha 3c, tubulin alpha 1b, tubulin alpha 3e, tubulin alpha 1c, tubulin beta 2B class IIb, tubulin, beta 2B class IIb, tubulin, beta 2B class IIB, tubulin, alpha 4A, tubulin alpha 4a, tubulin alpha like 3
NCBI Gene Aliases2410129E14Rik, RGD1309427, K-ALPHA-1, TUBA3, TUBA2, TUBA1, LIS3, TUBA6, Tuba4, PMGYSA, Tuba2, Tuba1, Tuba6, RGD1565476, brdp, bA408E5.3, bA506K6.1, M[a]6, M[a]4, CDCBM7, Tubb2, Tuba-1, ALS22, bcm948, B-ALPHA-1, H2-ALPHA
ClonalityMonoclonal
Concentration0.5 mg/mL
ModificationsAcetylated: Lys40
Storage Temperature-20°C
PurityProtein A
Antibody TypePrimary
Storage-20°C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Cancer cell employs a microenvironmental neural signal trans-activating nucleus-mitochondria coordination to acquire stemnessSignal transduction and targeted therapyJuly 19, 2023He B, Gao R, Lv S, Chen A, Huang J, Wang L, Feng Y, Feng J, Liu B, Lei J, et al.α-Tom20, 1:200, Proteintech, Cat# 11802-1-AP, RRID: AB_2207530, 1:200; α-Ac-α-tubulin, 1:200, Cat# 32-2700, RRID: AB_2533073, 1:200) overnight at 4°C. Next, the cells were exposed to secondary antibodies conjugated

(2) Degradomic Identification of Membrane Type 1-Matrix Metalloproteinase as an ADAMTS9 and ADAMTS20 SubstrateMolecular & cellular proteomics : MCPJune 1, 2023Nandadasa S, Martin D, Deshpande G, Robert KL, Stack MS, Itoh Y, Apte SS.Primary cilia were visualized by staining with anti-acetylated alpha tubulin (1:400, Invitrogen, catalog no. 32-2700) and imaged using a Zeiss Axioplan microscope equipped with a Leica DMC6200 camera. Cilium length and

(3) Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the ratPLOS ONEApril 24, 2023Andrea Cetina-Palma, Carmen Namorado-Tónix, Rafael Rodríguez-Muñoz, Paula Vergara, José Luis Reyes-Sánchez, José Segovia(Cat. #A-21442, A-11062), anti-Alexa Fluor 488 (Cat. #A-11055, A-11001) and anti-alpha tubulin (Cat. #32–2700) were obtained from Invitrogen (Carlsbad, CA, USA). Anti-CD24 antibody (Cat. #551133) was obtained from

(4) High-Throughput Quantitative Screening of Glucose-Stimulated Insulin Secretion and Insulin Content Using Automated MALDI-TOF Mass SpectrometryCellsMarch 9, 2023Clément Philippe Delannoy, Egon Heuson, Adrien Herledan, Frederik Oger, Bryan Thiroux, Mickaël Chevalier, Xavier Gromada, Laure Rolland, Philippe Froguel, Benoit Deprez, et al.antibodies directed against PDX1 (1:1000, Abcam, ab47267, Cambridge, UK), alpha-tubulin (1:1000, Invitrogen, 32-2700, Waltham, MA, USA), washed three times with PBS-0.05% tween and followed by incubation with secondary

(5) A therapeutic target for CKD: activin A facilitates TGFβ1 profibrotic signalingCellular & molecular biology lettersJanuary 30, 2023Soomro A, Khajehei M, Li R, O'Neil K, Zhang D, Gao B, MacDonald M, Kakoki M, Krepinsky JC.ab31013), TRII (Abcam; ab78419), ALK4 (Abcam, ab109300) PDGFR (Cedarlane; 1469-1), tubulin (Invitrogen; 32-2700) and lamin B (Santa Cruz; ac-6217). ImageJ was used to quantify band intensity. The following horseradish

(6) Bromo-protopine, a novel protopine derivative, alleviates tau pathology by activating chaperone-mediated autophagy for Alzheimerâ€™s disease therapyFrontiers in Molecular BiosciencesOctober 26, 2022Sravan Gopalkrishnashetty Sreenivasmurthy, Ashok Iyaswamy, Senthilkumar Krishnamoorthi, Rambabu N. Reddi, Ananth Kumar Kammala, Karthick Vasudevan, Sanjib Senapati, Zhou Zhu, Cheng-Fu Su, Jia Liu, et al.States",# AFIB0042,"WB 1:1,000" Acetylated a-tubulin (Lys40),"Thermoscientific, Waltham, MA′ UT",# 32–2700,WB 1:20000 α-tubulin,"Abcam, Waltham, MA, United States",# ab18251,WB 1:20000 ACTB (β-actin),"Santa

(7) TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopyCell Rep MethodsOctober 24, 2022Steib E, Tetley R, Laine RF, Norris DP, Mao Y, Vermot J.mono.",IGBMC,"Cat#ACT-2D7, alpha actin" "Actin, Rabbit poly.",Sigma,Cat#A2066 Acetylated tubulin,Thermofisher,Cat#32-2700; RRID:AB_2533073 "Alpha-tubulin, Mouse mono.",Life technologies,Cat#62204; RRID:AB_1965960 "Beta-tubulin,

(8) Vestibular Hair Cells Require CAMSAP3, a Microtubule Minus-End Regulator, for Formation of Normal KinociliaFrontiers in Cellular NeuroscienceJune 17, 2022Josephine O’Donnell, Jing Zhenganti-Camsap3 (Robinson et al., 2020) at 1:2500, anti-acetylated tubulin at 1:500 (Thermo Fisher Scientific 32-2700, AB_2533073). Samples were incubated with primary antibodies at 4°C overnight. The next day samples

(9) First Immunodetection of Sensory and Nervous Systems of Parasitic Larvae (Glochidia) of Freshwater Bivalve Nodularia douglasiaeFrontiers in PhysiologyApril 11, 2022Viktoria E. Nikishchenko, Elena M. Sayenko, Vyacheslav A. Dyachukused an acetyl-alpha tubulin (Lys40) monoclonal antibody (clone 6-11B-1 from Thermo Fisher, Cat. No. # 32-2700). Acetylation of α-tubulin is a modification in different microtubule structures and various eukaryotic cells

(10) Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral LineFrontiers in cell and developmental biologyJanuary 1, 2022Lin MJ, Lee CM, Hsu WL, Chen BC, Lee SJ.1:100), rabbit anti-GFP antibody (GTX113617, GeneTex, 1:500), mouse anti-tubulin (acetyl Lys40) antibody (32-2700, Thermo Scientific, 1:1000), and rat anti-mCherry antibody (M11217, Molecular probes, Thermo Scientific,

(11) Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profilesScientific ReportsDecember 1, 2021Emily J. Ross et al.proteins) (Thermo 339100) and acetylated alpha-tubulin (enabling imagining of primary cilia) (Thermo 32-2700). Chemicals Albumin–fluorescein isothiocyanate conjugate (A9771), Valspodar (PSC833) (SML0572), and

(12) Loss of Sprouty Produces a Ciliopathic Skeletal Phenotype in Mice Through Upregulation of Hedgehog SignalingJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchNovember 1, 2021Hruba E, Kavkova M, Dalecka L, Macholan M, Zikmund T, Varecha M, Bosakova M, Kaiser J, Krejci P, Hovorakova M, et al.

(13) Heterotrimeric Kinesin II is required for flagellar assembly and elongation of nuclear morphology during spermiogenesis in Schmidtea mediterraneaDevelopmental BiologySeptember 1, 2021Donovan A. Christman, Haley N. Curry, Labib Rouhanaanti-acetylated-α-tubulin (Lys40) monoclonal antibodies (clone 6-11B-1; Thermo Fisher, Waltham, MA; Catalog No. 32-2700; 1:400 dilution) for 2 hours at 4°C the following day. Lobes were incubated in primary antibody with

(14) Targeting an anchored phosphatase-deacetylase unit restores renal ciliary homeostasiseLifeJuly 12, 2021Gopalan J, Omar MH, Roy A, Cruz NM, Falcone J, Jones KN, Forbush KA, Himmelfarb J, Freedman BS, Scott JD.rabbit polyclonal,1:1000), GAPDH (Novus, mouse monoclonal, 1:5000), Acetylated tubulin (Thermo Fisher # 32–2700, 1:1000), HDAC6 (Proteintech # 12834–1-AP, 1:1000). Antibodies used for immunofluorescence: Acetylated

(15) Adenine nucleotide translocase regulates airway epithelial metabolism, surface hydration and ciliary functionJournal of cell scienceFebruary 25, 2021Kliment CR, Nguyen JMK, Kaltreider MJ, Lu Y, Claypool SM, Radder JE, Sciurba FC, Zhang Y, Gregory AD, Iglesias PA, et al.

(16) Sept8/SEPTIN8 involvement in cellular structure and kidney damage is identified by genetic mapping and a novel human tubule hypoxic modelScientific reportsJanuary 22, 2021Keele GR, Prokop JW, He H, Holl K, Littrell J, Deal AW, Kim Y, Kyle PB, Attipoe E, Johnson AC, et al.3% BSA at 1:200. These included ZO-1 (ThermoFisher, #33-9100), Acetyl-alpha Tubulin (ThermoFisher, #32-2700), and SEPTIN8 (ThermoFisher, # PA531356). Secondary antibodies (1:1000) against mouse (ThermoFisher,

(17) Changes in microtubule stability in zebrafish (Danio rerio) embryos after glyphosate exposureHeliyonJanuary 1, 2021Díaz-Martín RD, Valencia-Hernández JD, Betancourt-Lozano M, Yáñez-Rivera B.Sigma-Aldrich, while the anti-GAPDH antibody (MA5-15738) and the anti-acetyl-alpha (α) Tubulin (Lys40) antibody (32–2700) were obtained from Thermo Fisher Scientific (San Jose, USA). The secondary antibodies conjugated to

(18) Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T CellsCell metabolismDecember 1, 2020Wu B, Qiu J, Zhao TV, Wang Y, Maeda T, Goronzy IN, Akiyama M, Ohtsuki S, Jin K, Tian L, et al.gamma,Abcam,"Cat#ab25101, RRID:AB_448613" Mouse monoclonal anti-Acetyl-alpha Tubulin (Lys40),Thermo Fisher Scientific,"Cat#32–2700, RRID:AB_2533073" Rabbit monoclonal anti-alpha Tubulin,Cell Signaling Technology,"Cat#2125, RRID:AB_2619646" Rabbit

(19) Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signalingEMBO molecular medicineNovember 6, 2020Bosakova M, Abraham SP, Nita A, Hruba E, Buchtova M, Taylor SP, Duran I, Martin J, Svozilova K, Barta T, et al.fixed in paraformaldehyde and incubated with the following antibodies: acetylated α‐tubulin (1:100; 32‐2700; Invitrogen), ARL13B (1:300; 17711‐1‐AP; Proteintech), pericentrin (1:1,000; ab4448; Abcam), γ‐tubulin

(20) Dynein assembly factor with WD repeat domains 1 (DAW1) is required for the function of motile cilia in the planarian Schmidtea mediterraneaDevelopment, Growth & DifferentiationAugust 1, 2020Sydney Lynn Lesko, Labib Rouhanaanti-acetyl-alpha-tubulin (Lys40) monoclonal antibodies (1:400 dilution; clone 6-11B-1; Catalog No. 32-2700, Thermo Fisher, Waltham, Massachusetts, USA) and DAPI staining of cell nuclei as previously described

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Acetyl-alpha Tubulin (Lys40) Antibody

Acetyl-alpha Tubulin (Lys40) Antibody from Thermo Fisher Scientific

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Acetyl-alpha Tubulin (Lys40) Antibody from Thermo Fisher Scientific

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