Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 A-31571 from Thermo Fisher Scientific

Product Specs
ItemDonkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA-31571
Quantity1 mg
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry
Conjugate/TagAlexa Fluor™ 647
FormatPBS with 5mM sodium azide; pH 7.5
ReactivityMouse
TargetMouse IgG (H+L) Highly Cross-Adsorbed
IsotypeIgG
HostDonkey
Antibody TypeSecondary
ImmunogenGamma Immunoglobins Heavy and Light chains
ClonalityPolyclonal
Concentration2 mg/mL
Storage Temperature4° C, store in dark
Storage4° C, store in dark

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Thermo Fisher Scientific

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United States

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Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Aryl hydrocarbon receptor sulfenylation promotes glycogenolysis and rescues cancer chemoresistanceJ Clin InvestDecember 15, 2023Nannan Zhou, Jie Chen, Zheng Ling, Chaoqi Zhang, Yabo Zhou, Dianheng Wang, Li Zhou, Zhenfeng Wang, Nan Sun, Xin Wang, et al.Fluor 594 donkey anti-rabbit IgG(H+L) (A21207, Invitrogen), Alexa Fluor 647 donkey anti-mouse IgG(H+L) (A31571, Invitrogen), and Alexa Fluor 647 goat anti-rabbit IgG(H+L) (A21245, Invitrogen). Finally, after 3 PBS

(2) Late gene therapy limits the restoration of retinal function in a mouse model of retinitis pigmentosaNat CommunDecember 12, 2023Miranda L. Scalabrino, Mishek Thapa, Tian Wang, Alapakkam P. Sampath, Jeannie Chen, Greg D. Fieldgenerously provided by R. Molday)49, Alexa Fluor donkey anti mouse 647 (1:500, Thermo Fisher Scientific Cat# A-31571, RRID:AB_162542), and Alexa Fluor donkey anti rabbit 555 (Thermo Fisher Scientific Cat# A-31572, RRID:AB_162543). All

(3) Hippocampus Insulin Receptors Regulate Episodic and Spatial Memory Through Excitatory/Inhibitory BalanceASN NeuroNovember 1, 2023Cai-Yan Xue, Tian Gao, E Mao, Zhen-Zhen Kou, Ling Dong, Feng GaoRRID:AB_2535792, Invitrogen, CA, 1:500), and Alexa 647 donkey anti-mouse (Thermo Fisher Scientific Cat# A-31571, RRID:AB_162542, Invitrogen, CA, 1:500). Finally, the sections were rinsed with PBS three times, mounted

(4) Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female miceeBioMedicineNovember 1, 2023Mauro S.B. Silva, Laurine Decoster, Gaspard Delpouve, Tori Lhomme, Gaetan Ternier, Vincent Prevot, Paolo Giacobinidonkey anti-rabbit IgG (Invitrogen; #A10042) and Alexa Fluor 647 donkey anti-mouse IgG (Invitrogen; A-31571), both at 1:500 dilution, under rotation at 37 °C for five days. This was followed by three washes in

(5) Stem cell proliferation and differentiation during larval metamorphosis of the model tapeworm Hymenolepis microstomaFrontiers in Cellular and Infection MicrobiologyOctober 16, 2023Jimena Montagne, Matías Preza, Uriel KoziolAlexa Fluor 546 (Invitrogen A11010) and anti-mouse antibodies conjugated to Alexa Fluor 647 (Invitrogen A31571). Histological sectioning and immunofluorescence For some experiments, immunofluorescence was performed

(6) Biological Activity In Vitro, Absorption, BBB Penetration, and Tolerability of Nanoformulation of BT44:RET Agonist with Disease-Modifying Potential for the Treatment of NeurodegenerationBiomacromoleculesOctober 9, 2023Malik Salman Haider, Arun Kumar Mahato, Anastasiia Kotliarova, Stefan Forster, Bettina Böttcher, Philipp Stahlhut, Yulia Sidorova, Robert LuxenhoferFluor 647 conjugated donkey antimouse secondary antibody (1:500, Thermo Fisher Scientific, USA, Cat# A-31571) and 0.2 μg/mL of DAPI (4′,6-diamidino-2-phenylindole) for 1 h at room temperature. Finally, cells were

(7) Unique functional responses differentially map onto genetic subtypes of dopamine neuronsNature NeuroscienceOctober 1, 2023Maite Azcorra, Zachary Gaertner, Connor Davidson, Qianzi He, Hailey Kim, Shivathmihai Nagappan, Cooper K. Hayes, Charu Ramakrishnan, Lief Fenno, Yoon Seok Kim, et al.(Molecular Probes, A-11055, RRID:AB_2534102), donkey anti-mouse Alexa Fluor 647 (Thermo Fisher Scientific, A-31571, RRID:AB_162542), donkey anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific, A-31573, RRID:AB_2536183),

(8) Customization of the translational complex regulates mRNA-specific translation to control CNS regenerationNeuronSeptember 20, 2023Schaeffer J, Vilallongue N, Decourt C, Blot B, El Bakdouri N, Plissonnier E, Excoffier B, Paccard A, Diaz JJ, Humbert S, et al.,Thermo Fisher Scientific,Cat# A11004; RRID: AB_2534072 Donkey anti-mouse Alexa Fluor 647,Thermo Fisher Scientific,Cat# A31571; RRID: AB_162542 Donkey anti-rabbit Alexa Fluor 488,Thermo Fisher Scientific,Cat# A21206; RRID: AB_2535792 Donkey

(9) Involvement of neuronal tachykinin-like receptor at 86C in Drosophila disc repair via regulation of kynurenine metabolismiScienceSeptember 15, 2023Soshiro Kashio, Shu Masuda, Masayuki Miura488",Thermo Fisher Science,Cat#A-21208; RRID: AB_2535794 "Anti-mouse-IgG, 647",Thermo Fisher Science,Cat#A-31571; RRID: AB_162542 "Anti-rabbit-IgG, 647",Thermo Fisher Science,Cat#A-31573; RRID: AB_2536183 "Anti-mouse-IgG,

(10) Rewiring the pneumococcal capsule pathway for investigating glycosyltransferase specificity and genetic glycoengineeringScience advancesSeptember 8, 2023Su T, Chua WZ, Liu Y, Fan J, Tan SY, Yang DW, Sham LT.Alexa Fluor 647–conjugated donkey anti-mouse immunoglobulin G (IgG) antibody (Thermo Fisher Scientific, A-31571) at a 1:10,000 dilution. Blots were washed again three times with PBST and imaged. To detect the α-1,3–linked

(11) Identification of an alternative triglyceride biosynthesis pathwayNatureSeptember 7, 2023Gian-Luca McLelland, Marta Lopez-Osias, Cristy R. C. Verzijl, Brecht D. Ellenbroek, Rafaela A. Oliveira, Nicolaas J. Boon, Marleen Dekker, Lisa G. van den Hengel, Rahmen Ali, Hans Janssen, et al.568- and 647-conjugated secondary antibodies (Invitrogen A10042, A11011, A11004, A11008, A21245 and A31571) and/or neutral lipid dyes, diluted 1:500 in blocking buffer, for 1 h at room temperature. Cells were

(12) Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson's disease modelsHuman molecular geneticsSeptember 5, 2023Williamson MG, Madureira M, McGuinness W, Heon-Roberts R, Mock ED, Naidoo K, Cramb KML, Caiazza MC, Malpartida AB, Lavelle M, et al.anti-rat Alexa Fluor 594 (Invitrogen, a21209) and donkey anti-mouse Alexa Fluor-647 (Thermo Fisher, a31571). The Mito-QC construct (32) was kindly provided by Dr Ian Ganley (School of Life Sciences, The University

(13) Protocol for automated multivariate quantitative-image-based cytometry analysis by fluorescence microscopy of asynchronous adherent cellsSTAR ProtocolsSeptember 1, 2023Laetitia Besse, Typhaine Rumiac, Anne Reynaud-Angelin, Cédric Messaoudi, Marie-Noëlle Soler, Sarah A.E. Lambert, Vincent Pennaneachanti-Mouse IgG (H + L) Alexa Fluor 647 conjugated, use at 1:500 dilution",Thermo Fisher Scientific,Cat# A-31571; RRID: AB_162542 "Peroxidase-AffiniPure Goat Anti-Mouse IgG (H + L), use at 1:10 000 dilution",Jackson ImmunoResearch

(14) Poly(GR) interacts with key stress granule factors promoting its assembly into cytoplasmic inclusionsCell reportsAugust 29, 2023Park J, Wu Y, Shao W, Gendron TF, van der Spek SJF, Sultanakhmetov G, Basu A, Castellanos Otero P, Jones CJ, Jansen-West K, et al.(Goat anti-Rabbit IgG (H+L)),Invitrogen,Cat#A11034; RRID:AB_2576217 AlexaFluor 647 (Donkey anti-Mouse IgG (H+L)),Invitrogen,Cat#A31571; RRID:AB_162542 AlexaFluor 647 (Donkey anti-Rabbit IgG (H+L)),Invitrogen,Cat#A31573; RRID:AB_2536183 ,, Bacterial

(15) PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid modelCell reportsAugust 29, 2023Rakotomamonjy J, Rylaarsdam L, Fares-Taie L, McDermott S, Davies D, Yang G, Fagbemi F, Epstein M, Fairbanks-Santana M, Rozet JM, et al."Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647",ThermoFisher Scientific,"Cat #: A-31571, RRID: AB_162542" "Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™

(16) A comparative study of in vitro air-liquid interface culture models of the human airway epithelium evaluating cellular heterogeneity and gene expression at single cell resolutionRespiratory researchAugust 28, 2023Prescott RA, Pankow AP, de Vries M, Crosse KM, Patel RS, Alu M, Loomis C, Torres V, Koralov S, Ivanova E, et al.donkey anti-rabbit 647 (ThermoFisher, cat. no. A31573) or donkey anti-mouse 647 (ThermoFisher, cat. no. A31571). Slides were incubated in secondary for one hour at room temperature. Slides were washed twice with

(17) Calcium Signaling during Cortical Apical Dendrite Initiation: A Role for Cajal-Retzius NeuronsInt J Mol SciAugust 19, 2023Joshua R. Enck, Eric C. OlsonThermoFisher), AlexaFluor donkey anti-rabbit (#A31573, Thermofisher), AlexaFluor donkey anti-mouse 647 (#A31571, ThermoFisher). Secondary antibodies were diluted 1:500 in blocking buffer. The nuclear stain Hoechst

(18) Fibroblasts generate topographical cues that steer cancer cell migrationSci AdvAugust 18, 2023Francesco Baschieri, Abigail Illand, Jorge Barbazan, Olivier Zajac, Clémence Henon, Damarys Loew, Florent Dingli, Danijela Matic Vignjevic, Sandrine Lévêque-Fort, Guillaume MontagnacSigma-Aldrich. Alexa-conjugated secondary antibodies anti-mouse-A488 (ref. A21208), anti-mouse-A647 (ref. A31571), anti-rabbit-A647 (ref. A31573), and anti-rabbit-A488 (ref. A21206) were from Molecular Probes (Invitrogen).

(19) Organoid cell fate dynamics in space and timeSci AdvAugust 18, 2023Xuan Zheng, Max A. Betjes, Pascal Ender, Yvonne J. Goos, Guizela Huelsz-Prince, Hans Clevers, Jeroen S. van Zon, Sander J. Tans(1:1000; Abcam, #ab150166), Donkey Anti-Mouse IgG H&L (Alexa Fluor 647) (1:500; Thermo Fisher Scientific, #A31571), and Donkey Anti-Rabbit IgG H&L (Alexa Fluor 405) preadsorbed (1:1000; Abcam, #ab175649). The dyes

(20) Cohesin‐mediated DNA loop extrusion resolves sister chromatids in G2 phaseThe EMBO JournalAugust 15, 2023Paul Batty, Christoph CH Langer, Zsuzsanna Takács, Wen Tang, Claudia Blaukopf, Jan‐Michael Peters, Daniel W Gerlich1:250) and visualised with a donkey anti‐mouse Alexa Fluor 647 secondary antibody (Molecular Probes, A31571, 1:1,000). SCC1/RAD21 was detected with a monoclonal mouse antibody (Millipore, 05‐908, 1:500) and visualised

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(1)

5/7/2020Best Anti-Mouse 647 Secondary Antibody Abhijit NirwaneWe routinely use this donkey anti mouse secondary antibody to label various mouse primary antibodies and detect them at Cy5 channel.
(1)

6/16/2015Good anti-mouse CD11b antibody Tima ThomasThe objective of this experiment was to identify the percentage of CD11b positive cells in the splenocytes isolated from Balb/C mouse. To do this, single cell suspensions isolated from splenocytes ... Read Review
(1)

3/5/2015Donkey Anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 Conjugate Monica ValenciaThis is a great product to do immunofluorescence. We study primary cells (airway epithelial cells) in our lab and can use several different donkey alexas with different fluorophore conjugations in a ... Read Review

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Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 from Thermo Fisher Scientific

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Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 from Thermo Fisher Scientific

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