beta Actin Antibody AM4302 from Thermo Fisher Scientific

Product Specs
Itembeta Actin Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAM4302
Quantity100 µg
ApplicationsImmunocytochemistry, Western Blot
Conjugate/TagUnconjugated
Formatascites with 15mM sodium azide
ReactivityBovine, Canine, Chicken, Drosophila, Feline, Guinea Pig, Human, Ovine, Porcine, Rabbit
Targetbeta Actin
IsotypeIgG1
CloneAC-15
HostMouse
ImmunogenA slightly modified synthetic beta-cytoplasmic actin N-terminal peptide,Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH
NCBI Full Gene Nameactin beta, actin, beta
NCBI Gene AliasesBRWS1, ACT-5, Bact, actin, PS1TP5BP1
ClonalityMonoclonal
Storage TemperatureMaintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Antibody TypePrimary
StorageMaintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Bacteroides Fragilis in the gut microbiomes of Alzheimer’s disease activates microglia and triggers pathogenesis in neuronal C/EBPβ transgenic miceNature CommunicationsSeptember 6, 2023Yiyuan Xia, Yifan Xiao, Zhi-Hao Wang, Xia Liu, Ashfaqul M. Alam, John P. Haran, Beth A. McCormick, Xiji Shu, Xiaochuan Wang, Keqiang Yefor WB" Anti-LTB4-R2,Thermo-fisher,"Cat# BS-2655R, 1:1000 for WB" Anti-β actin (AC-15),Invitrogen,"Cat# AM4302, 1:1000 for WB" Anti-mouse IgG-HRP,Cell Signaling Technology,"Cat# 7076 S, 1:2000 for WB" Anti-rabbit

(2) Phytochemical mediated modulation of COX-3 and NFκB for the management and treatment of arthritisSci RepAugust 21, 2023Dipak Biswas, Bharat Gopalrao Somkuwar, Jagat Chandra Borah, Pritish Kumar Varadwaj, Saurabh Gupta, Zeeshan Ahmad Khan, Gopinath Mondal, Asamanja Chattoraj, Lokesh Debtargeting COX-3 (Santa Cruz, 1:1000 dilution), NFκB (p65) (Santa Cruz, 1:1000 dilution) or β-actin (Ambion-AM4302, 1:5000 dilution, control). The membranes were then washed with TBST buffer and incubated for 1 h at

(3) Berberine Reduces Lipid Accumulation in Obesity via Mediating Transcriptional Function of PPARδInternational Journal of Molecular SciencesJuly 18, 2023Jia-Wen Shou, Pang-Chui ShawPPARα (GTX101098, GeneTex), PPARγ (2435S, Cell Signaling), HO-1 antibody (GeneTex, GTX101147), β-Actin (AM4302, Thermo Fisher, Waltham, MA, USA), goat anti-rabbit IgG (H + L)-HRP conjugate (98164S, Cell Signaling),

(4) Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast CancerBreast Cancer: Basic and Clinical ResearchJuly 5, 2023Shokufeh Razi, Hossein Mozdarani, Roudabeh Behzadi Andouhjerdiβ-actin, as an internal control, was detected with antibeta-actin monoclonal antibody (1:1000) (Cat AM4302, Thermo Fisher Scientific). Statistical analysis All reactions were run in duplicates. The amplification

(5) A non-coding variant in the Kozak sequence of RARS2 strongly decreases protein levels and causes pontocerebellar hypoplasiaBMC Medical GenomicsJune 21, 2023Romain Nicolle, Nami Altin, Karine Siquier-Pernet, Sherlina Salignac, Pierre Blanc, Arnold Munnich, Christine Bole-Feysot, Valérie Malan, Barthélémy Caron, Patrick Nitschké, et al.diluted in OdysseyTM Blocking Buffer: anti-RARS2 (1:16000, ab230274, Abcam), Anti-β Actin (1:10000, AM4302, Invitrogen). After three washes with 0.2% PBST, the membranes were then incubated for 1 h at room temperature

(6) Faulty Metabolism: A Potential Instigator of an Aggressive Phenotype in Cdk5-dependent Medullary Thyroid CarcinomabioRxivJune 20, 2023Priyanka Gupta, Brendon Herring, Nilesh Kumar, Rahul Telange, Sandra S. Garcia-Buntley, Tessa W. Caceres, Simona Colantonio, Ford Williams, Pradeep Kurup, Angela M. Carter, et al.(Bibb Lab; 1:1000), FAM53C (Invitrogen PA5-114093; RRID:AB_2884608; 1:1000) and β-actin (Invitrogen AM4302; RRID:AB_437394; 1:5000). For immunostaining, tissues were fixed in formalin, embedded in paraffin,

(7) Injury-induced activation of the endocannabinoid system promotes axon regenerationiScienceJune 16, 2023Martinez-Torres S, Mesquida-Veny F, Del Rio JA, Hervera A.Cat# 4370, RRID:AB_2315112" Mouse monoclonal anti- beta Actin,Thermo Scientific,"Clone:AC-15, Cat# AM4302, RRID: AB_2536382" ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(8) PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell ComplexNature communicationsJune 15, 2023Wang Y, Chiappetta G, Guérois R, Liu Y, Romero S, Boesch DJ, Krause M, Dessalles CA, Babataheri A, Barakat AI, et al.1:1000); anti-GAPDH (Thermo Fisher Scientific, AM4300, 1:4000); anti-β-actin (Thermo Fisher Scientific, AM4302, 1:4000); and anti-GM130 (#610823, BD Biosciences, 1:100). Home-made CYFIP1, ABI1, WAVE2 antibodies

(9) Distinct Nrf2 Signaling Thresholds Mediate Lung Tumor Initiation and ProgressionCancer ResearchJune 15, 2023Janine M. DeBlasi, Aimee Falzone, Samantha Caldwell, Nicolas Prieto-Farigua, Justin R. Prigge, Edward E. Schmidt, Iok In Christine Chio, Florian A. Karreth, Gina M. DeNicolaRRID:AB_778944, 1:1,000), GSR (Santa Cruz Biotechnology, RRID:AB_2295121, 1:1,000), β-actin (Invitrogen AM4302, RRID:AB_2536382, 1:100,000). HRP secondary antibodies used include goat anti-rabbit IgG (Jackson ImmunoResearch

(10) DDIT4 Downregulation by siRNA Approach Increases the Activity of Proteins Regulating Fatty Acid Metabolism upon Aspirin Treatment in Human Breast Cancer CellsCurrent Issues in Molecular BiologyMay 28, 2023Aistė Savukaitytė, Agnė Bartnykaitė, Justina Bekampytė, Rasa Ugenskienė, Elona Juozaitytėobtained from Abcam (Cambridge, UK). Anti-phospho-ACC1 Ser79 (#PA5-17725) antibody and anti-β-actin (#AM4302) were purchased from Invitrogen (Waltham, MA, USA). Horseradish peroxidase-conjugated anti-rabbit (#65-6120),

(11) m(6)A-modification of cyclin D1 and c-myc IRESs in glioblastoma controls ITAF activity and resistance to mTOR inhibitionCancer lettersMay 28, 2023Benavides-Serrato A, Saunders JT, Kumar S, Holmes B, Benavides KE, Bashir MT, Nishimura RN, Gera J.Bethyl Laboratories), YTHDC2 (#35440S, CST), cyclin D1 (#2922, CST), c-myc (#9402, CST), and actin (#AM4302, ThermoFisher). PP242 and rapamycin were obtained from LC Laboratories (Woburn, MA). All other reagents

(12) Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virusiScienceMay 19, 2023Chen DY, Turcinovic J, Feng S, Kenney DJ, Chin CV, Choudhary MC, Conway HL, Semaan M, Close BJ, Tavares AH, et al.Invitrogen (#02–6202), Anti-VSV-G antibody from Kerafast (#EB0010), and ß-actin antibody from Invitrogen (#AM4302). Plasmids Expression plasmid encoding the spike protein of SARS-CoV-2, pCG1_SARS-2_S, has recently

(13) Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m(3)C modificationCell stem cellMarch 2, 2023Zhang F, Yoon K, Zhang DY, Kim NS, Ming GL, Song H.,Cell Signaling Technology,Cat#3873S; RRID: AB_1904178 Mouse monoclonal anti-β-actin,Thermo Fisher Scientific,Cat#AM4302; RRID: AB_2536382 "Donkey polyclonal anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,

(14) SerpinA3N deficiency attenuates steatosis and enhances insulin signaling in male miceJournal of EndocrinologyMarch 1, 2023Melanie Tran, Md Golam Mostofa, Michael Picard, Jianguo Wu, Li Wang, Dong-Ju Shin(sc-365062, Santa Cruz Biotechnology Inc.), TUBULIN (sc-5286, Santa Cruz Biotechnology Inc.) and β-ACTIN (AM4302, Thermo Fisher Scientific). The membranes were incubated in the corresponding HRP conjugated rabbit

(15) Omega-Class Glutathione Transferases Protect DNA from Oxidative Stress in Pathogenic Helminth Reproductive CellsAntioxidants (Basel)February 23, 2023Jeong-Geun Kim, Insug Kang, Chun-Seob Ahn, Woon-Mok Sohn, Yoon Kong1D4, catalog No. MA1-16757, ThermoFisher; 1:200 dilution), and anti-β-actin (clone AC-15, catalog No. AM4302, ThermoFisher; 1:400 dilution). All images were obtained after a 2 min exposure for quantitative analysis. 4.11.

(16) Identification of protein candidates in spermatozoa of water buffalo (Bubalus bubalis) bulls helps in predicting their fertility statusFrontiers in Cell and Developmental BiologyFebruary 20, 2023Seema Karanwal, Ankit Pal, Jatinder Singh Chera, Vipul Batra, Arumugam Kumaresan, Tirtha K. Datta, Rakesh Kumarincubated with primary antibodies namely, mouse anti-β-actin (Thermo Fisher Scientific, catalogue no. AM4302, 1:20,000 dilution), rabbit anti-Sp17 (Invitrogen, catalogue no. PA5-69981, 1:15,000 dilution), goat

(17) Ejaculatory Abstinence Affects the Sperm Quality in Normozoospermic Men—How Does the Seminal Bacteriome Respond?International Journal of Molecular SciencesFebruary 9, 2023Eva Tvrdá, Michal Ďuračka, Filip Benko, Anton Kováčik, Daniel Lovíšek, Eliška Gálová, Jana Žiarovská, Marianna Schwarzová, Miroslava KačániováUSA); Polyclonal/IgG; source: rabbit; 1:1000 in 5% milk in TBS/0.1% Tween-20.β-actin antibody (AC-15) (#AM4302; Invitrogen, Waltham, MA, USA); Monoclonal/IgG; source: mouse; 1:1000 in 5% milk in TBS/0.1% Tween-20. The

(18) mRNA ageing shapes the Cap2 methylome in mammalian mRNANatureFebruary 1, 2023Despic V, Jaffrey SR.anti-GAPDH monoclonal antibody (1:10,000; GT239, GeneTex), mouse anti-β-actin monoclonal antibody (1:4,000; AM4302, Thermo), horseradish peroxidase-conjugated donkey anti-rabbit IgG (1:5,000; NA934, Cytiva) and horseradish

(19) Genetic tools for the stable overexpression of circular RNAsRNA BiologyDecember 31, 2022Nicol Mecozzi, Arianna Nenci, Olga Vera, Ilah Bok, Aimee Falzone, Gina M. DeNicola, Florian A. Karrethanti-GFP (1:1,000; Cell Signalling Technologies, Cat. # 2956) or anti-Actin (1:4,000; Invitrogen, Cat. # AM4302) antibodies diluted in 5% non-fat dry milk in TBS-T. The membranes were washed with TBS-T and incubated

(20) β1 integrin monoclonal antibody treatment ameliorates cerebral cavernous malformationsFASEB journal : official publication of the Federation of American Societies for Experimental BiologyDecember 1, 2022McCurdy S, Lin J, Shenkar R, Moore T, Lightle R, Faurobert E, Lopez-Ramirez MA, Awad I, Ginsberg MH.

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6/9/2017Beta Actin Levels In Rat Lung Madhoosudan PatilThe adult rat lung with and without lung injury was loaded on the SDS-PAGE and blotted for beta actin antibody. It has been found that the levels of the protein does not change with the acute lung ... Read Review

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beta Actin Antibody

beta Actin Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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beta Actin Antibody from Thermo Fisher Scientific

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