Fig 2: The CRL4Cdt2 E3 ligase complex is important for MVM replication. A) Schematic showing the experimental protocol for figures 2B and 2C. B and C) Upper panels: murine A9 cells treated with siRNA as shown in 2A were infected at an MOI of 0.5, harvested at the indicated time points and processed for Southern blotting using an MVM genomic probe. Rf - replicative forms. SS - single stranded genomic DNA. Representative Southern Blots are shown; quantifications in the text reflect two DDB1 and three Cdt2 separate knockdown experiments. Lower panels: western blots show knockdown of DDB1 and Cdt2 done in parallel experiments under identical conditions to replication assays.

Fig 3: The CRL4Cdt2 ligase is recruited to viral replication compartments. A and B) Murine A9 cells were mock infected or infected with MVM at an MOI of 10. At 24 hr pi cells were pre-extracted using cytoskeletal buffer and processed for IF using antibodies against NS1 and DDB1 (A) or Cdt2 (B).