alpha Tubulin Antibody Alexa Fluor 488 322588 from Thermo Fisher Scientific

Product Specs
Itemalpha Tubulin Antibody Alexa Fluor 488
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number322588
Quantity100 µg
ApplicationsImmunocytochemistry
Conjugate/TagAlexa Fluor™ 488
FormatPBS with 4mg/mL BSA and 0.1% sodium azide; pH 7.4
ReactivityHuman, Mouse, Rat, Sea urchin
Targetalpha Tubulin
IsotypeIgG1 kappa
CloneB-5-1-2
HostMouse
ImmunogenSarkosyl-resistant filament from sea urchin sperm axonemes
ClonalityMonoclonal
Concentration0.5 mg/mL
Storage TemperatureMaintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
NCBI Full Gene Nametubulin, alpha-like 3, tubulin, alpha 1A, tubulin, alpha 1C, tubulin, alpha 1B, tubulin alpha 1a, tubulin alpha 3c, tubulin alpha 1b, tubulin alpha 3e, tubulin alpha 1c, tubulin beta 2B class IIb, tubulin, beta 2B class IIb, tubulin, beta 2B class IIB, tubulin, alpha 4A, tubulin alpha 4a, tubulin alpha like 3
NCBI Gene Aliases2410129E14Rik, RGD1309427, K-ALPHA-1, TUBA3, TUBA2, TUBA1, LIS3, TUBA6, Tuba4, PMGYSA, Tuba2, Tuba1, Tuba6, RGD1565476, brdp, bA408E5.3, bA506K6.1, M[a]6, M[a]4, CDCBM7, Tubb2, Tuba-1, ALS22, bcm948, B-ALPHA-1, H2-ALPHA
PurityProtein A
Antibody TypePrimary
StorageMaintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

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Thermo Fisher Scientific

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Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Xrcc5/KU80 is not required for the survival or activation of prophase-arrested oocytes in primordial folliclesFront Endocrinol (Lausanne)October 10, 2023Natasha D. Ratnayaka-Gamage, Lauren R. Alesi, Nadeen Zerafa, Jessica M. Stringer, Karla J. Huttat room temperature. Subsequently, oocytes were incubated with 1/100 anti-alpha tubulin (Invitrogen, 322588) 1/100 phalloidin (F-actin) (Invitrogen, 872816) and 1/5000 Hoechst (Invitrogen, 879457) in 0.1% BSA

(2) NLRP14 Safeguards Calcium Homeostasis via Regulating the K27 Ubiquitination of Nclx in Oocyte‐to‐Embryo TransitionAdvanced ScienceSeptember 1, 2023Tie‐Gang Meng, Jia‐Ni Guo, Liu Zhu, Yike Yin, Feng Wang, Zhi‐Ming Han, Lei Lei, Xue‐Shan Ma, Yue Xue, Wei Yue, et al.(for IF, ABclonal, AE005), anti‐FLAG(R) M2 Magnetic Beads (Sigma, M8823), anti‐tubulin‐F488 (Thermo, 322588), anti‐Centromere Protein Antibody (for IF, Antibodies Inc, 15–234), anti‐mUHRF1 (for WB and IF, Santa Cruz

(3) CFAP300 mutation causing primary ciliary dyskinesia in FinlandFrontiers in GeneticsSeptember 30, 2022RÃ¼diger Schultz, Varpu Elenius, Mahmoud R. Fassad, Grace Freke, Andrew Rogers, Amelia Shoemark, Tiina Koistinen, Mai A. Mohamed, Jacqueline S. Y. Lim, Hannah M. Mitchison, et al.CFAP300 (c11orf70, HPA038585, Sigma-Aldrich) antibodies and colocalized with cilia marker alpha-tubulin (322588, Invitrogen). After washes with PBST the slides were incubated with secondary antibodies Alexa Fluor

(4) Toxic effects of AZD1208 on mouse oocytes and its possible mechanismsJournal of cellular physiologySeptember 1, 2022Yan FZ, Ouyang YC, Meng TG, Zhang HY, Yue W, Zhang XR, Xue Y, Wang ZB, Sun QY.

(5) Depletion of oocyte dynamin-related protein 1 shows maternal-effect abnormalities in embryonic developmentScience advancesJune 17, 2022Adhikari D, Lee IW, Al-Zubaidi U, Liu J, Zhang QH, Yuen WS, He L, Winstanley Y, Sesaki H, Mann JR, et al.incubated with Alexa Fluor 488–conjugated mouse anti–α-tubulin (1:200; Thermo Fisher Scientific no. 322588) for 1 hour at RT. After three washes, chromosomes were labeled using Hoechst 33342 (10 μg/ml; Sigma-Aldrich)

(6) Using ZINC08918027 inhibitor to determine Aurora kinase-chromosomal passenger complex isoforms in mouse oocytesBMC research notesMarch 7, 2022Kratka C, Drutovic D, Blengini CS, Schindler K.antibodies were as follows: mouse anti α-tubulin Alexa-fluor 488 conjugated (1:100, Life Technologies #322588), rabbit anti-pINCENP (1:1000, gift from M. Lampson, University of Pennsylvania; [22]) and anti-centromeric

(7) Microtubule organizing centers regulate spindle positioning in mouse oocytesDevelopmental cellJanuary 24, 2022Londoño-Vásquez D, Rodriguez-Lukey K, Behura SK, Balboula AZ.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies α-tubulin-Alexa Fluor 488 conjugate,Life Technologies,Cat# 322588; RRID: AB_1502303 Pericentrin,BD Biosciences,Cat# 611814; RRID: AB_399294 CREST autoimmune serum,Antibodies Incorporated

(8) Miro1 depletion disrupts spatial distribution of mitochondria and leads to oocyte maturation defectsFrontiers in cell and developmental biologyJanuary 1, 2022Lee IW, Adhikari D, Carroll J.temperature. Meiotic spindles were stained using an Alexa fluor conjugated anti-ɑ-Tubulin antibody (#322588, Invitrogen) at a concentration of 1/200 in 1% BSA. DNA was labeled by Hoechst 33342 (10 μg/ml; Sigma-Aldrich)

(9) Age-dependent integrity of the meiotic spindle assembly checkpoint in females requires Aurora kinase BAging cellNovember 1, 2021Blengini CS, Nguyen AL, Aboelenain M, Schindler K.Antibodies Incorporated #15‐234), Mouse anti α‐Tubulin Alexa Fluor 488 conjugated (IF:1:100, Life Technologies #322588), Rabbit anti‐ MAD2 (IF:1:1000, IB: 1:500; Biolegend #924601 or previously Covance #PRB‐452C)), sheep

(10) Tubulin acetylation increases cytoskeletal stiffness to regulate mechanotransduction in striated muscleThe Journal of general physiologyJuly 5, 2021Coleman AK, Joca HC, Shi G, Lederer WJ, Ward CW.nitrocellulose membrane and blocked (5% milk). The membrane was probed with primary antibodies for α-tubulin (322588, clone B-5-1-2; Thermo Fisher Scientific), acetylated (T7451, clone 6-11B-1; Sigma-Aldrich), or detyrosinated

(11) Aurora kinase A is essential for meiosis in mouse oocytesPLoS geneticsApril 1, 2021Blengini CS, Ibrahimian P, Vaskovicova M, Drutovic D, Solc P, Schindler K.immunofluorescence (IF) experiments: mouse anti ⍺-tubulin Alexa-fluor 488 conjugated (1:100; Life Technologies #322588) AURKA (1:500; Bethyl #A300-072A), ACA (1:30; Antibodies Incorporated #15–234), phosphorylated CDC25B

(12) Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic OocyteFrontiers in cell and developmental biologyJanuary 1, 2021Zhang HY, Li J, Ouyang YC, Meng TG, Zhang CH, Yue W, Sun QY, Qian WP.oocytes were incubated overnight at 4°C with 1:800 anti-α-tubulin–fluorescein isothiocyanate antibody (322588, Thermo Fisher Scientific). Next, oocytes were washed three times with PBS containing 0.1% Tween-20

(13) In vitro 3D culture lung model from expanded primary cystic fibrosis human airway cellsJournal of Cystic FibrosisSeptember 1, 2020Rachael E. Rayner, Jack Wellmerling, Wissam Osman, Sean Honesty, Maria Alfaro, Mark E. Peeples, Estelle Cormet-Boyakaand sectioned for immunofluorescence staining. Antibodies used included acetylated-α-tubulin (B-5-1-2#322588 Alexa Fluor-488 conjugated, Invitrogen, 1:500), MUC5AC (clone 45M1 #M5293 Sigma Aldrich, 1:100), MUC5B

(14) Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in mice†Biology of reproductionAugust 4, 2020Devlin DJ, Nozawa K, Ikawa M, Matzuk MM.ProteinTech, Chicago, IL, USA), and mouse mAb anti-α-Tubulin-Alexa Fluor 488 (1:200 dilution, product no. 322588, Invitrogen) were purchased and used for immunostaining. Rabbit pAb anti-FAM170B (1:50 dilution, product

(15) Knockout of mouse receptor accessory protein 6 leads to sperm function and morphology defects†Biology of reproductionMay 26, 2020Devlin DJ, Agrawal Zaneveld S, Nozawa K, Han X, Moye AR, Liang Q, Harnish JM, Matzuk MM, Chen R.

(16) NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA DecayCell reportsMay 19, 2020Nourreddine S, Lavoie G, Paradis J, Ben El Kadhi K, Méant A, Aubert L, Grondin B, Gendron P, Chabot B, Bouvier M, et al.

(17) The cyclin B2/CDK1 complex inhibits separase activity in mouse oocyte meiosis IDevelopment (Cambridge, England)December 2, 2019Li J, Ouyang YC, Zhang CH, Qian WP, Sun QY.containing 1% BSA for 1 h at RT. The oocytes were incubated with a FITC-conjugated anti-α-tubulin antibody (322588, Thermo Fisher Scientific) overnight at 4°C. The following morning, oocytes were washed three times

(18) Loss of the E2 SUMO-conjugating enzyme Ube2i in oocytes during ovarian folliculogenesis causes infertility in miceDevelopment (Cambridge, England)December 2, 2019Rodriguez A, Briley SM, Patton BK, Tripurani SK, Rajapakshe K, Coarfa C, Rajkovic A, Andrieux A, Dejean A, Pangas SA.

(19) A Sexually Dimorphic Role for STAT3 in Sonic Hedgehog MedulloblastomaCancersNovember 1, 2019White CL, Jayasekara WSN, Picard D, Chen J, Watkins DN, Cain JE, Remke M, Gough DJ.alpha-tubulin directly conjugated to AlexaFluor 488 (1/200, Invitrogen clone B-5-1-2, ThermoFisher catalog # 322588). For immunohistochemistry, the Vectastain Elite ABC HRP kit (Vector Laboratories, Burlingame, CA, USA)

(20) Coupled Active Systems Encode an Emergent Hunting Behavior in the Unicellular Predator Lacrymaria olorCurrent BiologyNovember 1, 2019Scott M. Coyle, Eliott M. Flaum, Hongquan Li, Deepak Krishnamurthy, Manu PrakashRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Alexa Fluor 488 conjugated anti-alpha-tubulin antibody,Invitrogen,322588 Anti-centrin mouse monoclonal antibody,Milipore,04–1624 Alexa Fluor 568 conjugated anti-mouse IgG

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4/14/2017Good Anti-Alpha Tubulin Antibody Beckley DavisThis antibody (322588) was used to perform immunofluorescence microscopy on HeLa cells to detect endogenous microtubules. 322588 showed strong immunoreactivity that was consistent with microtubules. ... Read Review

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alpha Tubulin Antibody Alexa Fluor 488

alpha Tubulin Antibody Alexa Fluor 488 from Thermo Fisher Scientific

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alpha Tubulin Antibody Alexa Fluor 488 from Thermo Fisher Scientific

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