Fig 1: Effects of the PTZ kindling and vitamin administration on the brain expression of p53 protein and its phosphorylation after seizures, in comparison to the effects of the corresponding injections in the control rats. Seizures (S) are induced according to the two protocols shown in Figure 1: SA defines the animals treated by the protocol in Figure 1A, i.e., without the PTZ kindling (n = 4 without vitamins; n = 5 with vitamins); SB defines the animals subjected to the PTZ kindling according to the protocol in Figure 1B (n = 6 without vitamins; n = 6 with vitamins). In the control (C) animals, CA defines those receiving saline instead of vitamins and/or PTZ according to the protocol in Figure 1A (n = 5 without vitamins; n = 5 with vitamins); CB defines the analogous control animals additionally subjected to the repetitive injections of saline instead of PTZ according to the protocol in Figure 1B (n = 6 without vitamins; n = 7 with vitamins). Statistically significant (p < 0.05) differences between the experimental groups, determined using the two-way ANOVA with Tukey’s post hoc test, are shown on the graphs. Further, significant factors, determined by the two-way ANOVA, and their statistics, are indicated under the graphs. The levels of p53 or its phosphorylated at Ser392 form (pS392) are normalized to total protein in the lane and shown as a ratio to the levels in the comparison groups without vitamins (CA or SA) (see “Materials and Methods”). The original images of the blots are provided as Supplementary Figure S5.