Western blot analysis using the anti-TIP5 crude serum. Nuclear extracts (150 µg) from NIH3T3 or HeLa cells were analyzed by western blot using the anti-TIP5 crude serum (Product # 49-1037), diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween-20. The molecular weight marker is shown on the left, the location of the protein of interest is indicated on the right.