Fig 2: ALDH3A1 regulates tumor cell proliferation through p53/BAG1 axis. The correlation between ALDH3A1 and BAG1 (A) or TP53 (B) in GEPIA. (C) BAG1 expression in NC, ALDH3A1-sh1 and ALDH3A1-sh2 cell lines. (D) The expression of BAG1, p53 and ß-actin detected by western blot in NC, ALDH3A1-sh1 and ALDH3A1-sh2 cell lines. (E) ALDH3A1 and BAG1 expression in tumor cell lines treated with p53 inhibitor. (F) Tumor cell growth from A549 cell line transduced with BAG1-sh by CCK-8, and absorbance per well was measured at 450 nm using an automatic microplate reader. (G) MFI of Ki67 expression detected by flow cytometry in tumor cell transduced with BAG1-sh. (H) Representative images of invasion and statistical analysis of invasion cell numbers per field in A549 cell line transduced with BAG1-sh. Data were expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.