Fig 2: Frequency of CD34+, CD34+CD82+ and CD34+CD133+ cells in patients with ALL-ND and controls. (A) Frequency of CD34-positive cells in patients with ALL-ND was significantly increased compared with controls. (B) Frequency of CD34+CD82+ cells in patients with ALL-ND was significantly higher than in controls. (C) In 6 of 17 patients with ALL-ND, the frequency of CD34+CD133+ cells was >10%, whereas no control patients exhibited a frequency of CD34+CD133+ cells of 10%. CD, cluster of differentiation; ALL-ND, newly diagnosed acute lymphoblastic leukemia. **P<0.001 vs. controls.

Fig 3: mRNA expression of CD133 and CD82. (A) CD82 mRNA expression in ND was significantly higher than in controls. **P<0.001, ND vs. control (B) CD82 mRNA expression in B-ALL ND was significantly higher than in CR and controls. *P<0.05, ND vs. CR or ND vs. control (C) CD82 mRNA expression in T-ALL ND and CR was significantly higher than in controls. *P<0.05, ND vs. control or CR vs. control (D) CD133 mRNA expression was markedly higher in B-ALL ND compared with controls, and CD133 mRNA expression was significantly higher than in CR. *P<0.05, ND vs. CR. ND, newly-diagnosed; CR, complete remission; CD, cluster of differentiation; B-ALL, B cell-acute lymphoblastic leukemia; T-ALL, T cell-acute lymphoblastic leukemia.

Fig 4: Myosin heavy chain (MYH) subtypes and triad-associated genes display characteristic expression patterns. (A) The mRNA expression of the myosin heavy chain family (MYH2, MYH3, MYH4, MYH7, and MYH8) was quantified by real-time PCR. The expression levels of the MYH genes in PAX7-Venus iMuSC-derived myotubes (upper) and in CD57-–CD82+ iMuSC-derived myotubes (lower) on days 14 and 42 in c-condition (no Matrigel embedding) and m-condition (Matrigel embedding) are shown. (B) The mRNA expression of SR-related genes (Casq1 and Atp2a1) and t-tubule-related genes (DHPRa, DHPRß, and DHPR?) was quantified by real-time PCR. The expression levels of MYH genes in PAX7-Venus iMuSC-derived myotubes (upper) and in CD57-–CD82+ iMuSC-derived myotubes (lower) on days 14 and 42 in c-condition and m-condition are shown. The vertical axis of the graph shows the relative expression level normalized by 18S rRNA, the housekeeping gene. The expression level is standardized by the expression level (=1) on day 5. *p < 0.05, **p < 0.01; c-condition vs. m-condition. †p < 0.05, ††p < 0.01; day 14 vs. day 42 (unpaired two-tailed Student’s t-test). Error bars indicate the standard error of mean (SEM). In each group (day 14c, day 14m, day 42c, and day 42m), three independent samples were used for the analysis.

Fig 5: A positive correlation was observed between CD133 and CD82 mRNA expression in pediatric B cell-acute lymphoblastic leukemia. CD, cluster of differentiation.