Fig 2: Changes in the expression of genes regulating stress responses and cellular metabolism after E7386 treatment in CRC organoids. (A) Upregulation of CHAC1, ULBP1 and PCK2 expression associated with E7386 concentration 24 h after the addition of E7386 in CRC organoids. (B) Downregulation of FDPS and HDAC2 expression associated with E7386 concentration 24 h after the addition of E7386 in CRC organoids. Results were obtained using DNA microarray (correlation coefficient >0.7; left) and reverse transcription-quantitative PCR (right). Data are presented as the mean ± SD. **P<0.01. The color of asterisks represents significance compared to the corresponding 0 nM controls in single culture (blue) or co-culture (red) groups. CRC, colorectal cancer. CHAC1, glutathione-specific ?-glutamylcyclotransferase 1; ULBP1, UL16 binding protein 1; PCK2, phosphoenolpyruvate carboxykinase 2; FDPS, farnesyl diphosphate synthase; HDAC2, histone deacetylase 2.