Fig 1: CD8+T cells are the main source of IFN? in the tumour and surrounding milieu.(a) Percentages of NK, NKT, CD8+T and CD4+T cells among CD45+IFN-?+ cells isolated from the tumour and (b) surrounding tissue of DEN-treated Klrk1+/+ and Klrk1-/-mice, AMC and YC (n=2-8) upon 4 h in vitro stimulation with PMA & Ionomycin. Graphs represent the mean±s.e.m. (c) Representative flow plots and (d) average percentages (mean±s.e.m.) of CD8+T cells expressing PD-1 and/or IFN? post in vitro stimulation as described for (a). (e) Percentages of CD8+T, (f) NK and (g) NKT cells expressing CD107a (n=2–7). Graphs represent the mean±s.e.m. (h) Percentages of CD107a+ cells among CD8+T cells expressing NKG2D (NKG2D+) or not (NKG2D-) in DEN-treated Klrk1+/+ mice, AMC and YC. Dots represent populations from individual mice. Lines connect populations from the same mouse. (i) Relative expression of perforin and (j) granzyme B transcripts within tumour (T) and surrounding (S) tissue of Klrk1+/+ and Klrk1-/- DEN-treated mice (n=19) and AMC liver (n=9). Graphs represent the mean±s.e.m. Statistical analyses were performed by unpaired Student's t-test. Statistically significant differences between groups are denoted as: *P=0.05 and **P=0.01.