Anti-EpCAM antibody ab71916 from Abcam

Product Specs
ItemAnti-EpCAM antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab71916
Antibody TypePrimary
Quantity100 UG
IsotypeIgG
ClonalityPolyclonal
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaImmuno-oncology
Gene NameEPCAM
ReactivityHuman, Mouse, Rat
TargetEpCAM

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Chemically induced reprogramming to reverse cellular agingAging (Albany NY)July 15, 2023Jae-Hyun Yang, Christopher A. Petty, Thomas Dixon-McDougall, Maria Vina Lopez, Alexander Tyshkovskiy, Sun Maybury-Lewis, Xiao Tian, Nabilah Ibrahim, Zhili Chen, Patrick T. Griffin, et al.following concentrations in 1% BSA in PBST: NANOG (Invitrogen, PA5-85110) 1:200, and EPCAM (Abcam, ab71916) 1:100. Primary antibodies were incubated for 1 hour at room temperature followed by three washes of

(2) NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in miceNature communicationsJune 26, 2023Tran LS, Ying L, D'Costa K, Wray-McCann G, Kerr G, Le L, Allison CC, Ferrand J, Chaudhry H, Emery J, et al.containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916; Abcam, Boston, MA, USA). The antibodies used in this study are listed in Supplementary Table 1. An

(3) Assessment of drug permeability through an ex vivo porcine round window membrane modeliScienceJune 16, 2023Adele Moatti, Dylan Silkstone, Taylor Martin, Keith Abbey, Kendall A Hutson, Douglas C Fitzpatrick, Carlton J Zdanski, Alan G Cheng, Frances S Ligler, Alon GreenbaumREAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, "Anit-EpCAM, CD326",Abcam,ab71916; RRID:AB_1603782 Anti-Vimentin,Santa Cruz Biotechnology,sc-6260; RRID:AB_628437 Goat anti-mouse AF

(4) Live-cell invasive phenotyping uncovers the ALK2/BMP6 iron homeostasis pathway as a therapeutic vulnerability in LKB1-mutant lung cancerbioRxiv : the preprint server for biologyJune 14, 2023Koo J, Seong CS, Parker RE, Dwivedi B, Arthur RA, Dinasarapu AR, Johnston HR, Claussen H, Tucker-Burden C, Ramalingam SS, et al.(ab108965), ID-1 (ab192303), Transferrin receptor (ab84036), SCD1 (ab236868), Ferritin (ab75973), EpCAM (ab71916) and TTF1 (ab76013) antibodies were from Abcam. KRAS (sc-30), p53 (sc-126 for human), a-Tubulin (sc-8035)

(5) Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model systemFrontiers in Veterinary ScienceMay 26, 2023Itsuma Nagao, Yoko M. Ambrosinianti-mouse E-cadherin antibody (36/E-cadherin, BD Biosciences), polyclonal anti-rabbit EpCAM antibody (ab71916, Abcam), monoclonal anti-mouse MUC5AC antibody (45 M1, Thermo Fisher Scientific), and monoclonal anti-rabbit

(6) Reversine attenuates cholestatic ductular reaction in ratsFEBS Open BioMay 1, 2023Di Huang, Lijuan Tang, Tianyang Li, Peilin Li, Zisheng Huang, Jiefeng Weng, Shuai Zhang, Weili Gu, Yu HuangAbcam), anti‐CK7 (1 : 8000, ab181598; Abcam), anti‐CK19 (1 : 200, ab7755, Abcam), or anti‐EpCAM (1 : 200, ab71916; Abcam) antibodies, and then with horseradish peroxidase (HRP)‐conjugated rabbit anti‐mouse IgG (A9044;

(7) HIGD2A silencing impairs hepatocellular carcinoma growth via inhibiting mitochondrial function and the MAPK/ERK pathwayJournal of translational medicineApril 12, 2023Huang K, Liu Z, Xie Z, Li X, Zhang H, Chen Y, Wang Y, Lin Z, Li C, Liu H, et al.antibody (27733-1-AP, ProteinTech), anti-CD133 antibody (66666-1-Ig, ProteinTech), anti-EpCAM antibody (ab71916, Abcam), anti-JNK (9258, CST, USA), anti-pJNK (4668, CST), anti-Erk1/2 (4695, CST), anti-pErk1/2 (4377,

(8) Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defensesNature communicationsMarch 16, 2023Deng S, He W, Gong AY, Li M, Wang Y, Xia Z, Zhang XT, Huang Pacheco AS, Naqib A, Jenkins M, et al.anti-PCNA antibody (Cell Signaling, catalogue no. 13110, 1:1000), anti-EpCAM antibody (Abcam, catalogue no. ab71916, 1:200), anti-Lysozyme antibody (Abcam, catalogue no. ab108508, 1:500), anti-Villin antibody (Santa Cruz

(9) Human intestinal epithelial cells can internalize luminal fungi via LC3-associated phagocytosisFrontiers in ImmunologyMarch 8, 2023Sarit Cohen-Kedar, Efrat Shaham Barda, Keren Masha Rabinowitz, Danielle Keizer, Hanan Abu-Taha, Shoshana Schwartz, Kawsar Kaboub, Liran Baram, Eran Sadot, Ian White, et al.(VU-1D9),Mouse monoclonal,PFA or Methanol,1:400,187372,Abcam EpCAM,Rabbit polyclonal,PFA or Methanol,1:400,ab71916,Abcam anti mouse AF488,Donkey,,1:1000,ab150109,Abcam anti mouse AF647,Donkey,,1:1000,ab216773,Abcam anti

(10) Genomic heterogeneity in pancreatic cancer organoids and its stability with culturenpj Genomic MedicineDecember 19, 2022Olalekan H. Usman, Liting Zhang, Gengqiang Xie, Hemant M. Kocher, Chang-il Hwang, Yue Julia Wang, Xian Mallory, Jerome Iriantoovernight in the primary antibodies against pan-Keratin (Cell Signaling, #4545) and EpCAM (Abcam, #ab71916), both at 1:500 dilution. DNA was stained with 2 µg/mL DAPI (Sigma) for 15 min. Confocal imaging was

(11) Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysisGenome BiologyDecember 1, 2022Rui Wang, Yunuo Mao, Wendong Wang, Xin Zhou, Wei Wang, Shuai Gao, Jingyun Li, Lu Wen, Wei Fu, Fuchou TangAntibody,Source,Identifier,Dilution ratio EPCAM,Abcam,ab71916,1:200 CDX2,Abcam,ab76541,1:200 Villin (VIL1),Abcam,ab201989,1:200 CEACAM6,Abcam,ab134074,1:200 MKI67,Abcam,ab15580,1:200 SOX9,Abcam,ab185966,1:200 Vimentin

(12) Induced hepatic stem cells are suitable for human hepatocyte productioniScienceOctober 21, 2022Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara, Hirofumi NoguchiMAB97701-SP Anti beta-actin (C4),"Santa Cruz Biotechnology, Inc.",Cat# SC-47778 Human EpCAM aa 250,Abcam,Cat# ab71916 goat anti-Rabbit IgG H&L (Alexa Fluor 488),Abcam,Cat# ab150077 Anti HNF-4α (H-1) (Alexa Fluor 647),Santa Cruz Biotechnology

(13) Differential Radiosensitizing Effect of 50 nm Gold Nanoparticles in Two Cancer Cell LinesBiology (Basel)August 9, 2022Miguel Ángel Pérez-Amor, Leonardo Barrios, Gemma Armengol, Joan Francesc Barquineroimmunofluorescence staining was carried out. The primary antibodies used were Rabbit Anti-EpCam (cat. ab71916, Abcam, Cambridge, UK) and Mouse Anti-TfR (cat. ab9179, Abcam), and the secondary antibodies were Goat Anti-Rabbit IgG H&L Alexa Fluor® 488

(14) Development of Bovine Gastric Organoids as a Novel In Vitro Model to Study Host-Parasite Interactions in Gastrointestinal Nematode InfectionsFrontiers in Cellular and Infection MicrobiologyJune 30, 2022Marc N. Faber, David Smith, Daniel R. G. Price, Philip Steele, Katie A. Hildersley, Liam J. Morrison, Neil A. Mabbott, Alasdair J. Nisbet, Tom N. McNeillyat 4°C. The primary antibodies used to stain for epithelial markers were polyclonal rabbit α-EPCAM (ab71916, Abcam, used at a 1:600 dilution) and monoclonal mouse α-pan cytokeratin (used at a 1:100 dilution),

(15) Anti-Cancer Effects of YAP Inhibitor (CA3) in Combination with Sorafenib against Hepatocellular Carcinoma (HCC) in Patient-Derived Multicellular Tumor Spheroid Models (MCTS)Cancers (Basel)May 31, 2022Sojung Han, Ji Yeon Lim, Kyungjoo Cho, Hye Won Lee, Jun Yong Park, Simon Weonsang Ro, Kyung Sik Kim, Haeng Ran Seo, Do Young KimThe following primary antibodies were purchased: anti-YAP/TAZ (8418, Cell Signaling) and anti-EpCAM (ab71916, Abcam). Anti-EpCAM was used and for internal control, an anti-GAPDH antibody (2118, Cell Signaling)

(16) Tumor-Derived Exosomes Regulate Apoptosis of CD45+EpCAM+ Cells in Lung CancerFrontiers in ImmunologyMay 30, 2022Shixiang Lu et al.and 0.05% Tween 20 (P9416; Sigma-Aldrich). The sections were then incubated with anti-EpCAM antibody (ab71916; Abcam; 1:100). Subsequently, Cy3-conjugated goat anti-rabbit IgG (GB21303; Servicebio; 1:300) and FITC

(17) ESR1 mutant breast cancers show elevated basal cytokeratins and immune activationNature communicationsApril 19, 2022Li Z, McGinn O, Wu Y, Bahreini A, Priedigkeit NM, Ding K, Onkar S, Lampenfeld C, Sartorius CA, Miller L, et al.Abcam), CK17 (rabbit, 1:100, ab109725, Abcam), PR (M3569, mouse, 1:100, Agilent) EpCAM (rabbit, 1:100, ab71916, Abcam), CD45 (rabbit, 1:200, ab10558, Abcam), or S100A8/9 (mouse, 1:200, NBP1-60157, Novus Biologicals,

(18) Identification of Potential Key Genes Associated with Ovarian Clear Cell CarcinomaSSRN Electronic JournalMarch 30, 2022Youzheng Xu, Keng Shento SPP1, RRID: ab8448; rabbit polyclonal to DCN, RRID: ab175404; rabbit polyclonal to EPCAM, RRID: ab71916; rabbit monoclonal to ALDH1A1, RRID: ab52492; rabbit polyclonal to Gata6, RRID: ab22600; and rabbit

(19) Exploiting maleimide-functionalized hyaluronan hydrogels to test cellular responses to physical and biochemical stimuliBiomedical MaterialsMarch 1, 2022Andrea Mazzocchi, Kyung Min Yoo, Kylie G Nairon, L Madison Kirk, Elaheh Rahbar, Shay Soker, Aleksander Skardalof each were prepared in triplicate for staining as described above. The primary antibody for EpCam (ab71916, Abcam) was diluted 1:200 in antibody diluent and incubated overnight at 4 °C. Washing, the addition

(20) EpCAM Is Essential to Maintaining the Immune Homeostasis of Intestines via Keeping the Expression of pIgR in the Intestinal Epithelium of MiceFrontiers in immunologyJanuary 1, 2022Lei Z, Liu W, Nie Y, Yang Y, Chen G, Huang L, Wu H, Lei Y, Chen L, Hu Q, et al.with 1% BSA at room temperature for 1 hour. The primary antibodies included rabbit anti-EpCAM (1:200; ab71916; Abcam), rabbit anti-mouse IgA secondary antibody (1:1000; NB7506; Novus) and rabbit anti-Claudin-7

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Anti-EpCAM antibody from Abcam

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Anti-EpCAM antibody from Abcam

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