Fig 1: Intracellular localization of LLO-lacZ secreted ß-gal.The presence of ß-gal in the nuclei of host cells was determined by measuring fluorescent signals of anti-ß-gal in the nuclei compared to background signals as a ratio (SNR). Fluorescence microscopy (top) and SNR (bottom) of the following: J774A.1 cells with no LLO strain (none), J774A.1 cells incubated with ß-gal collected as supernatant from induced LLO-lacZ-NLS (supernatant), J774A.1 cells incubated with uninduced LLO-lacZ-NLS (-mannose), J774A.1 cells incubated with induced LLO-lacZ-no NLS (mannose -NLS) and J774A.1 cells incubated with induced LLO-lacZ-NLS (mannose NLS). Plotted data is mean ± SD from n = 50 random individuals from a representative experiment; ******p < 0.000001. Scale bars = 20 µm.