Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] ab80039 from Abcam

Product Specs
ItemAnti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab80039
Antibody TypePrimary
Clone34.2
IsotypeIgG2a
Quantity50 UG
Conjugate/TagUnconjugated
HostMouse, Nude mouse
ClonalityMonoclonal
ApplicationsWestern blot
Concentration1 mg/ml
Gene NamePRKAA1
ReactivityDrosophila melanogaster, Human, Rat
TargetAMPK alpha 1 + AMPK alpha 2

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Metabolic shift and the effect of mitochondrial respiration on the osteogenic differentiation of dental pulp stem cellsPeerJApril 21, 2023Lingyun Wan, Linyan Wang, Ran Cheng, Li Cheng, Tao Humouse anti-human, BioLegend, 982402), CD34 (PE, mouse anti-human, BioLegend, 343505), AMPK (Abcam, ab80039), p-AMPK (Abcam, ab133448), anti-GAPDH (Abcam, ab9485,ab8245), goat anti-rabbit IgG (H + L)-HRP (Bio-Rad Laboratories

(2) Fenofibrate Attenuates Renal Tubular Cell Apoptosis by Up-Regulating MCAD in Diabetic Kidney DiseaseDrug design, development and therapyJanuary 1, 2023Tang C, Deng X, Qu J, Miao Y, Tian L, Zhang M, Li X, Sun B, Chen L.Caspase 3/p17/p19 (1:1000, Proteintech, No.19677-1-AP), p-AMPK (1:2000, Abcam, ab23875), AMPK (1:2000, Abcam, ab80039), MCAD (1:10,000, Abcam, ab92461), FOXA2 (1:1000, Proteintech, No.22474-1-AP), H3 (1:10,000, Abcam,

(3) Icariside II, a Naturally Occurring SIRT3 Agonist, Protects against Myocardial Infarction through the AMPK/PGC-1α/Apoptosis Signaling PathwayAntioxidants (Basel, Switzerland)July 27, 2022Li Y, Feng L, Xie D, Lin M, Li Y, Chen N, Yang D, Gao J, Zhu Y, Gong Q.incubated with the following primary antibodies: p-AMPK (1:2000, #ab133448, Abcam), AMPK (1:1000, #ab80039, Abcam), PGC-1α (1:1000, #ab191838, Abcam), SIRT3 (1:1000, #ab264041, Abcam), SOD2 (1:5000, #ab13533,

(4) Gestational Exercise Increases Male Offspring’s Maximal Workload Capacity Early in LifeInternational Journal of Molecular SciencesApril 1, 2022Jorge Beleza, Jelena Stevanović-Silva, Pedro Coxito, Hugo Rocha, Paulo Santos, António Ascensão, Joan Ramon Torrella, José Magalhãesreduce nonspecific binding. After incubation with the following primary antibodies: anti-AMPK (1:1000; ab80039); anti-PGC-1α (1:1000; ab54481); anti-TFAM (1:500; sc-23588); anti-OXPHOS cocktail (1:1000; Abcam, ab110413);

(5) Uncoupling protein 1 knockout aggravates isoproterenol-induced acute myocardial ischemia via AMPK/mTOR/PPARα pathways in ratsTransgenic ResearchFebruary 1, 2022Daorong Hou, Heling Fu, Yuan Zheng, Dan Lu, Yuanwu Ma, Yuan Yin, Lianfeng Zhang, Dan BaoMembranes were immunoblotted with primary antibodies against UCP1 (ab10983, Abcam, USA), AMPK α1 + AMPK α2 (ab80039, Abcam, USA), phosphor-AMPK α1 (T183) + AMPK α2 (T172) (ab133448, Abcam, USA), mTOR (2972S, Cell Signaling Technology

(6) Empagliflozin Inhibits Hepatic Gluconeogenesis and Increases Glycogen Synthesis by AMPK/CREB/GSK3β Signalling PathwayFrontiers in physiologyJanuary 1, 2022Yu X, Meng Z, Fang T, Liu X, Cheng Y, Xu L, Liu X, Li X, Xue M, Li T, et al.with primary antibodies at 4°C overnight. The antibodies are listed as follows: total-AMPK (Abcam, ab80039, 1:2000, Cambridge, United Kingdom), phospho-AMPK at Thr172 (Abcam, ab23875, 1:2000, Cambridge, United

(7) Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axisJournal of InflammationDecember 20, 2021Pan Wu, Xiao Yu, Yue Peng, Qian-Lu Wang, Long-Tian Deng, Wei XingFactor A (Tfam) (ab119684, 1:1000 dilution, Abcam), SIRT1 (ab110304, 1:3000 dilution, Abcam), AMPK (ab80039, 1:3000 dilution, Abcam), Acetyl-CoA Carboxylase (ACC) (sc-137,104, 1:2000 dilution, Santa Cruz), p-ACC

(8) Qiangji Jianli Decoction Alleviates Hydrogen Peroxide-Induced Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics and Biogenesis in L6 MyoblastsOxidative Medicine and Cellular LongevityApril 13, 2021Jingwei Song et al.was purchased from Beyotime Biotechnology Co., Ltd (Shanghai, China). Primary antibodies AMPK (CAS: ab80039), p-AMPK (CAS: ab133448), PGC-1α (CAS: ab54481), NRF1 (CAS: ab175932), TFAM (CAS: ab131607), and GAPDH

(9) Emodin Ameliorates Renal Damage and Podocyte Injury in a Rat Model of Diabetic Nephropathy via Regulating AMPK/mTOR-Mediated Autophagy Signaling PathwayDiabetes, Metabolic Syndrome and Obesity: Targets and TherapyMarch 18, 2021Hong Liu, Quan Wang, Ge Shi, Wenqiang Yang, Yanmin Zhang, Weidong Chen, Sheng Wan, Fei Xiong, Zengsi WangWestern blot analysis was performed using the following antibodies: anti-rat nephrin (ab58968), anti-rat AMPK (ab80039), anti-rat p-AMPK (ab23875), anti-rat β-actin (ab227387) antibodies were from Abcam Ltd, HKSP, New Territories,

(10) Mitochondrial Function, Fatty Acid Metabolism, and Body Composition in the Hyperbilirubinemic Gunn RatFrontiers in PharmacologyMarch 8, 2021Josif Vidimce, Johara Pillay, Nirajan Shrestha, Lan-feng Dong, Jiri Neuzil, Karl-Heinz Wagner, Olivia Jane Holland, Andrew Cameron Bulmer(subunits of mitochondrial respiratory complexes, #ab110413 (Abcam); p-AMPK, #ab133448 (Abcam); AMPK, #ab80039 (Abcam); GAPDH, #14C10 (Cell Signaling, Australia)) with gentle agitation overnight at 4°C. Finally,

(11) Stilbene glycoside upregulates SIRT3/AMPK to promotes neuronal mitochondrial autophagy and inhibit apoptosis in ischemic strokeAdvances in Clinical and Experimental MedicineMarch 2, 2021Yuxian Li, Ke Hu, Minghua Liang, Qing Yan, Minjiang Huang, Ling Jin, Yuefu Chen, Xirong Yang, Xiaobo LiAbcam, Cambridge, USA), Bax (ab32503; Abcam), Bcl-2 (ab196495; Abcam), SIRT3 (ab189860; Abcam), AMPK (ab80039; Abcam), p-AMPK (ab23875; Abcam), LC3II/I (ab128025; Abcam), P62 (ab91526; Abcam), PINK1 (ab23707; Abcam),

(12) Autophagy impairment as a key feature for acetaminophen-induced ototoxicityCell death & diseaseJanuary 4, 2021Zhao T, Zheng T, Yu H, Hu BH, Hu B, Ma P, Yang Y, Yang N, Hu J, Cao T, et al.NB100-2220), anti-p62 (Abcam, ab56416), anti-p-AMPK (Cell Signaling Technology, #2531), anti-AMPK (Abcam, ab80039), anti-Hamartin/TSC1 (Cell Signaling Technology, #4906), anti-p-S6 (Ser235/236) (Cell Signaling Technology,

(13) AMPK‑SIRT1 pathway dysfunction contributes to neuron apoptosis and cognitive impairment induced by sevofluraneMolecular Medicine ReportsNovember 17, 2020Liwei Liu, Chao Liu, Lin Fangantibodies were purchased from Abcam: Anti‑actin (cat. no. ab179467), anti‑total‑(t‑) AMPK (cat. no. ab80039), anti‑p‑AMPK (cat. no. ab133448), and Breast Surgery, Shanghai Tenth People's Hospital, Tongji University

(14) AMPK/mTOR/ULK1 Axis-Mediated Pathway Participates in Apoptosis and Autophagy Induction by Oridonin in Colon Cancer DLD-1 CellsOncoTargets and TherapyAugust 25, 2020Heqi Bu, Dianlei Liu, Guolin Zhang, Li Chen, Zhangfa SongCell Signaling Technology, Inc. (Boston, USA); p-mTOR (Ser2448) (ab84400), mTOR (ab25880), p-AMPK (ab23875), AMPK (ab80039), p-ULK1 (ab256537), ULK1 (ab167139) were pur chased from Abcam (Cambridge, MA, USA). Goat anti-rabbit OncoTargets

(15) Kangtaizhi Granule Alleviated Nonalcoholic Fatty Liver Disease in High-Fat Diet-Fed Rats and HepG2 Cells via AMPK/mTOR Signaling PathwayJournal of Immunology ResearchAugust 20, 2020Jiaxin Zhang, Haixia Du, Menglan Shen, Zhengqi Zhao, Xinmiao Yeab8805, 1 : 500; FAS, ab82419, 1 : 1000; SIRT1, ab12193, 1 : 2000; p-AMPK, ab23875, 1 : 1000; AMPK, ab80039,1 : 1000; p-mTOR, ab131538, 1 : 1000; mTOR, ab32028, 1 : 5000; and GLUT2, ab192599, 1 : 5000 (Abcam, USA)

(16) Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitroFrontiers in pharmacologyJanuary 1, 2020Li T, Fang T, Xu L, Liu X, Li X, Xue M, Yu X, Sun B, Chen L.TET2 (1:1,000, Proteintech, No. 21207-1-AP), p-AMPK (1:2000, Abcam, ab23875), AMPK (1:2000, Abcam, ab80039), SGLT2 (1:1,000, Proteintech, No. 24654-1-AP), SREBP-1c (1:1,000, Abcam, ab28481), PPARα (1:800, Proteintech,

(17) The Potential Synergistic Modulation of AMPK by Lippia citriodora Compounds as a Target in Metabolic DisordersNutrientsDecember 4, 2019Mariló Olivares-Vicente et al.serum (Sigma-Aldrich). Then, cells were incubated with primary antibodies overnight, i.e., mouse anti-AMPK (ab80039, Abcam, Cambridge, UK) and rabbit anti-pAMPK (Thr172) (#2535, Cell Signaling Tech., Danvers, MA, USA),

(18) Histone deacetylase activity mediates thermal plasticity in zebrafish (Danio rerio)Scientific ReportsDecember 1, 2019Frank Seebacher, Alec I. M. SimmondsStudies Hybridoma Bank, University of Iowa), C5B11 (acetyl-H3K9; Cell Signaling, Danvers, MA, USA), ab80039 (total AMPK; Abcam, Cambridge, MA, USA), ab133448 (phosphorylated AMPK; Abcam, Cambridge, MA, USA),

(19) Metformin Suppresses Self-Renewal Ability and Tumorigenicity of Osteosarcoma Stem Cells via Reactive Oxygen Species-Mediated Apoptosis and AutophagyOxidative Medicine and Cellular LongevityNovember 18, 2019Bin Zhao, Jie Luo, Ye Wang, Liangfu Zhou, Jingmin Che, Fang Wang, Songlin Peng, Ge Zhang, Peng Shangc (ab133504), anti-LC3 (ab48394), anti-Oct4 (ab181557), anti-Sox2 (ab97959), anti-Ki67 (ab15580), anti-AMPK (ab80039), anti-phospho AMPK (ab133448), anti-mTOR (ab134903), anti-phospho mTOR (ab109268), CD105 (ab135528),

(20) Pioglitazone abrogates testicular damage induced by testicular torsion/detorsion in ratsIran J Basic Med SciOctober 4, 2019Nevertyty Mohamed Mahmoud, Soad Lotfy Kabilactivated protein kinase (AMPK) activity was estimated in testicular homogenates using anti-AMPK antibody (ab80039) ELISA assay kit (Abcam, Cambridge, UK), according to manufacturer’s instructions. Assay of TOS in serum Serum

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Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] from Abcam

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Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] from Abcam

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