PerCP/Cyanine5.5 anti-mouse CD3 100218 from BioLegend

Product Specs
ItemPerCP/Cyanine5.5 anti-mouse CD3
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100218
NCBI Gene AliasesCtg-3, Ctg3, T3g
Conjugate/TagPERCP_CYANINE5.5
HostRat
Quantity100 µg
ReactivityMouse
ApplicationsFlow Cytometry (FC)
Clone17A2
IsotypeIgG2b, Kappa
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
ClonalityMonoclonal
TargetCD3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A Rift Valley fever mRNA vaccine elicits strong immune responses in mice and rhesus macaquesnpj VaccinesOctober 27, 2023Ting Bian, Meng Hao, Xiaofan Zhao, Chuanyi Zhao, Gang Luo, Zhendong Zhang, Guangcheng Fu, Lu Yang, Yi Chen, Yudong Wang, et al.1:50), anti-CXCR5 PE/Cyanine7 (cat. number 145516, 1:50) and anti-CD3 PerCP/Cyanine5.5 (cat. number 100218, 1:150), which were all purchased from BioLegend. Next, the cells were fixed, resuspended in PBS, and

(2) Pre-existing CD4 T cell help boosts antibody responses but has limited impact on germinal center, antigen-specific B cell frequencies after influenza infectionFrontiers in ImmunologyAugust 30, 2023Danica F. Besavilla, Laura Reusch, Josue Enriquez, Karin Schön, Davide Angelettiused for extracellular T-cell characterization: 1:200 anti-mouse CD3 Per-CP-Cy5.5 (BioLegend, catalog: 100218), 1:100 anti-mouse CD4 APC-Cy7 (BioLegend, catalog: 100526), 1:100 anti-mouse CD69 BV605 (BioLegend,

(3) Enhancing autophagy in CD11c+ antigen-presenting cells as a therapeutic strategy for acute respiratory distress syndromeCell ReportsAugust 16, 2023Christine Quach, Doumet Georges Helou, Meng Li, Benjamin Pierre Hurrell, Emily Howard, Pedram Shafiei-Jahani, Pejman Soroosh, Jing-hsiung James Ou, Babak Razani, Virender Rehan, et al.,BD Bioscience,Cat # 552126; RRID: AB_394341 PerCP/Cyanine5.5 anti-mouse CD3 (clone 17A2),BioLegend,Cat # 100218; RRID: AB_1595492 Brilliant Violet 510 anti-mouse CD19 (Clone 6D5),BioLegend,Cat # 115546; RRID: AB_2562137 APC anti-mouse Ly-6G/Ly-6C (Gr-1

(4) Nanovesicles loaded with a TGF-β receptor 1 inhibitor overcome immune resistance to potentiate cancer immunotherapyNature CommunicationsJune 16, 2023Mengxue Zhou, Jiaxin Wang, Jiaxing Pan, Hui Wang, Lujia Huang, Bo Hou, Yi Lai, Fengyang Wang, Qingxiang Guan, Feng Wang, et al.cells were analyzed by flow cytometry. Anti-CD45-FITC (BD, 553079), anti-CD3-PerCP-Cy5.5 (BioLegend, 100218), anti-CD4-APC/Cy7 (BD, 552051), anti-CD8-PE (BD, 553032), anti-IFN-γ-APC (BD, 554413), anti-CD25-APC

(5) SARS-CoV-2 bivalent mRNA vaccine with broad protection against variants of concernFrontiers in ImmunologyMay 24, 2023Qinhai Ma, Man Li, Lin Ma, Caroline Zhang, Hong Zhang, Huiling Zhong, Jian Wen, Yongsheng Wang, Zewei Yan, Wei Xiong, et al.resuspended in FC buffer. Then, splenocytes were stained with PerCP/Cyanine5.5 anti-mouse CD3 (BioLegend, 100218), PE anti-mouse CD8a (BioLegend, 100708), APC anti-mouse CD4 (BioLegend, 100412), PE anti-mouse CD62L

(6) A small-molecule inhibitor of Keap1-Nrf2 interaction attenuates sepsis by selectively augmenting the antibacterial defence of macrophages at infection sitesEBioMedicineApril 1, 2023Wang Y, Tang B, Li H, Zheng J, Zhang C, Yang Z, Tan X, Luo P, Ma L, Wang Y, et al.anti mouse CD16/CD32 (#553141, BD Biosciences, RRID: AB_394656) and then stain with anti-mouse CD3 (#100218, Biolegend, RRID: AB_1595492), anti-mouse CD11b (#101205, Biolegend, RRID: AB_312788), anti-mouse Gr1

(7) Nanosecond Pulsed Electric Field Induces an Antitumor Effect in Triple-Negative Breast Cancer via CXCL9 Axis Dependence in MiceCancersMarch 30, 2023Xu Z, Pan C, Chen L, Qian J, Chen X, Zhou L, Zheng S.Biolegend were purchased for Zombie NIR Fixable Viability Kit (423106), CD45 (103112, 103140), CD3 (100218), CD4 (100510), CD8 (100706), CD11b (101245), F4/80 (123108) and CXCL9 (515606). The tumor was digested

(8) CARD11 gain-of-function mutation drives cell-autonomous accumulation of PD-1+ ICOShigh activated T cells, T-follicular, T-regulatory and T-follicular regulatory cellsFrontiers in ImmunologyMarch 7, 2023Etienne Masle-Farquhar, Yogesh Jeelall, Jacqueline White, Julia Bier, Elissa K. Deenick, Robert Brink, Keisuke Horikawa, Christopher Carl Goodnownumber,Antibody,Fluorochrome,Company,Clone 122007,CD28,FITC,BioLegend,E18 100204,CD3,FITC,BioLegend,17A2 100217,CD3,PerCP Cy5.5,BioLegend,17A2 563331,CD4,BV786,BD Biosciences,GK1.5 564306,CD4,BUV737,BD Biosciences,SK3 17-0051-81,CD5,APC,Thermo

(9) Itaconate ameliorates autoimmunity by modulating T cell imbalance via metabolic and epigenetic reprogrammingNature communicationsFebruary 27, 2023Aso K, Kono M, Kanda M, Kudo Y, Sakiyama K, Hisada R, Karino K, Ueda Y, Nakazawa D, Fujieda Y, et al.antibodies as follows: anti-mouse CD4 (clone H129.19, Biolegend, 130308; 1:100), CD3 (clone 17A2, Biolegend, 100217; 1:100), CD90.2 (clone 53-2.1, Biolegend, 140310; 1:100), CD11b (clone M1/70, BD Biosciences, 557657;

(10) Convection-enhanced delivery of nanoencapsulated gene locoregionally yielding ErbB2/Her2-specific CAR-macrophages for brainstem glioma immunotherapyJournal of NanobiotechnologyFebruary 20, 2023Lin Gao, Chongdeng Shi, Zhenmei Yang, Weiqiang Jing, Maosen Han, Jing Zhang, Cai Zhang, Chunwei Tang, Yuanmin Dong, Ying Liu, et al.fluorescence-labeled antibodies against CD45 (Biolegend, Cat. no. 103116, clone 30-F11), CD3 (Biolegend, Cat. no. 100217, clone 17A2), CD4 (Biolegend, Cat. no. 100429, clone GK1.5), CD8a (Biolegend, Cat. no. 100721, clone

(11) Combination of GD2-directed bispecific trifunctional antibody therapy with Pd-1 immune checkpoint blockade induces anti-neuroblastoma immunity in a syngeneic mouse modelFrontiers in ImmunologyJanuary 9, 2023Sara Marie Ivasko, Kathleen Anders, Laura Grunewald, Michael Launspach, Anika Klaus, Silke Schwiebert, Peter Ruf, Horst Lindhofer, Holger N. Lode, Lena Andersch, et al.assays) using LIVE/DEAD™ (Invitrogen) to gate out dead cells and antibodies against Cd3 (Biolegend, cat#100218), Cd4 (Biolegend, cat#1005599), Cd8a (Biolegend, cat#1000743), Cd69 (Biolegend, cat#104506), Pd-1 (Biolegend,

(12) Lactate oxidase/catalase-displaying nanoparticles efficiently consume lactate in the tumor microenvironment to effectively suppress tumor growthJournal of nanobiotechnologyJanuary 3, 2023Choi H, Yeo M, Kang Y, Kim HJ, Park SG, Jang E, Park SH, Kim E, Kang S.(100705, BioLegend), CD11b-PE-cy7 (101215, Biolegend), CD64-APC (139305, BioLegend), CD3-PerCP-cy5.5 (100217, BioLegend), NK1.1-APC-cy7 (108723, BioLegend), F4/80-FITC (123108, BioLegend), F4/80-APC (17-4801-82,

(13) Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channelsJournal of extracellular vesiclesDecember 1, 2022Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, et al.San Diego, CA, USA",103108, Percp/Cyanine5.5 Anti‐Mouse CD3 Antibody,"BioLegend, San Diego, CA, USA",100218, APC Anti‐Mouse CD4 Antibody,"BioLegend, San Diego, CA, USA",100412, PE/Cyanine7 Anti‐Mouse CD8a Antibody,"BioLegend,

(14) Cavitation assisted endoplasmic reticulum targeted sonodynamic droplets to enhanced anti-PD-L1 immunotherapy in pancreatic cancerJournal of NanobiotechnologyDecember 1, 2022Jifan Chen et al.(Biolegend, 423110), anti-mouse CD45 BV605 (Biolegend, 103139), anti-mouse CD3 PerCP/Cyanine5.5 (Biolegend, 100218), anti-mouse CD4 FITC (Biolegend, 100406), anti-mouse CD8 BV510 (Biolegend, 100752), anti-mouse CD49b

(15) Cladribine treatment improves cortical network functionality in a mouse model of autoimmune encephalomyelitisJ NeuroinflammationNovember 8, 2022Christina B. Schroeter, Leoni Rolfes, K. S. Sophie Gothan, Joel Gruchot, Alexander M. Herrmann, Stefanie Bock, Luca Fazio, Antonia Henes, Venu Narayanan, Steffen Pfeuffer, et al.Antigen,Reactivity,Supplier,Order no. Flow cytometry,Flow cytometry,Flow cytometry,Flow cytometry CD3,Mouse,BioLegend,100218 CD4,Mouse,BioLegend,100531 CD8a,Mouse,BioLegend,100730 CD11a,Mouse,BioLegend,153103 CD11b,Mouse/human,BioLegend,101263 CD11c,Mouse,BioLegend,117306 CD25,Mouse,BioLegend,101915 CD44,Mouse,BioLegend,103049 CD45,Mouse,BioLegend,103116 CD45R/B220,Mouse,BioLegend,103211 CD49d,Mouse,BioLegend,103627 CD62L,Mouse,BioLegend,104437 CD69,Mouse,BioLegend,104521 NK1.1,Mouse,BioLegend,108710 Antibodies

(16) Single-cell profiling reveals distinct adaptive immune hallmarks in MDA5+ dermatomyositis with therapeutic implicationsNature communicationsOctober 29, 2022Ye Y, Chen Z, Jiang S, Jia F, Li T, Lu X, Xue J, Lian X, Ma J, Hao P, et al.UCHT1, Biolegend), BUV395 anti-human CD3 (Cat: 564001, Clone: SK7, BD), PerCPCy5.5 anti-mouse CD3 (Cat: 100218, Clone: 17A2, Biolegend), APCCy7 anti-human CD4 (Cat: 357416, Clone: A161A1, Biolegend), Alexa Fluor

(17) Specific immunosuppressive role of nanodrugs targeting calcineurin in innate myeloid cellsiScienceOctober 21, 2022Miriam Colombo, Laura Marongiu, Francesca Mingozzi, Roberta Marzi, Clara Cigni, Fabio Alessandro Facchini, Rany Rotem, Mihai Valache, Giulia Stucchi, Giuseppe Rocca, et al.PE anti-mouse/human CD11b (clone M1/70),Biolegend,Cat. # 101208 Percp cy5.5 anti-mouse CD3 (clone 17A2),Biolegend,Cat. # 100218 PE anti-mouse CD4 (Clone GK1.5),Biolegend,Cat. # 100408 PEcy7 anti-mouse CD11c (clone N418),Biolegend,Cat.

(18) A Single Nucleotide Polymorphism in SH2B3/LNK Promotes Hypertension Development and Renal DamageCirculation ResearchOctober 14, 2022Matthew R. Alexander, Samuel Hank, Bethany L. Dale, Lauren Himmel, Xue Zhong, Charles D. Smart, Daniel J. Fehrenbach, Yuhan Chen, Nitin Prabakaran, Brian Tirado, et al.anti-CD45-BV750 (clone 30-F11; Biolegend; cat. 103157), anti-CD3-PerCPCy5.5 (clone 17A2; Biolegend; cat. 100217), anti-CD8-AF488 (clone 53–6.7; Biolegend; cat. 100726), and CD4-PacOrange (clone RM4–5; Invitrogen;

(19) Immunization with a Prefusion SARS-CoV-2 Spike Protein Vaccine (RBMRNA-176) Protects against Viral Challenge in Mice and Nonhuman PrimatesVaccines (Basel)October 11, 2022Qinhai Ma, Runfeng Li, Jianmin Guo, Man Li, Lin Ma, Jun Dai, Yongxia Shi, Jinlong Dai, Yuankeng Huang, Cailing Dai, et al.Cyto-Fast™ Fix/Perm Buffer Set (BioLegend, 426803). PerCP/Cyanine5.5 anti-mouse CD3 antibody (BioLegend, 100218), APC anti-mouse CD8a antibody (BioLegend, 100712), and APC anti-mouse CD4 antibody (BioLegend, 100412)

(20) Network and Experimental Pharmacology to Decode the Action of Wendan Decoction Against Generalized Anxiety DisorderDrug Design, Development and TherapySeptember 27, 2022Qi Jin et al.Chemical, USA), Acetonitrile (A955-1, Fisher Chemical, USA). PerCP/Cyanine5.5 anti-mouse CD3 Antibody (100218, BioLegend, USA), FITC anti-mouse CD4 Antibody (116004, BioLegend, USA), PE anti-mouse IL-6 Antibody

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2/19/2019Anti-Mouse CD3 PerCP/Cy5.5 Antibody Lakshmi BolluThis antibody was used in flow cytometry analysis to detect CD3-positive lymphocytes in spleen and tumor samples.
(1)

10/29/2018Anti-Mouse CD3 Antibody for Flow Cytometry Alexander RaymourOur lab studies immune responses to infection in mouse models. The detection of various immune cell subsets is important in delineating the immune responses in various tissue compartments. The use of ... Read Review
(1)

2/23/2017Anti-Mouse CD3 Antibody Anup KumarI am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.
(1)

3/22/2016Anti-Mouse CD3 Antibody Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-mycobacterial ESAT6 binding peptides.
(1)

11/5/2015Anti-CD3 Antibody With Low Level Fluorescence Gurpanna SagguPERCP-Cy5.5 is not the best channel, but was the only one available in my staining panel. So, I decided to use it for a lineage marker like anti-CD3. Usually, the expression is high enough to get a ... Read Review

PerCP/Cyanine5.5 anti-mouse CD3 from BioLegend

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PerCP/Cyanine5.5 anti-mouse CD3 from BioLegend

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